Summary: The genes encoding both subunits of the succinyl-CoA synthetase of have been identified as distal genes of the operon, which also encodes the dehydrogenase (Elo; ) and succinyltransferase (E20; ) components of the 2-oxoglutarate dehydrogenase complex. The newly defined genes express polypeptides of 41 kDa () and 31 kDa (), corresponding to the and subunits of succinyl-CoA synthetase, respectively. The genes are thus located at 16·8 min in the linkage map, together with the citrate synthase () and succinate dehydrogenase () genes, in a cluster of nine citric acid cycle genes: . Four deletion strains lacking all of these citric acid cycle enzymes were characterized. The succinyl-CoA synthetase activities of strains harbouring plasmids containing the and genes were amplified some fourfold. Further emzymological studies indicated that expression of succinyl-CoA synthetase is coordinately regulated with 2-oxoglutarate dehydrogenase.


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