Summary: The gene coding for ornithine carbamoyltransferase (OTCase) is not expressed in However, OTCase-deficient strains transformed with plasmids carrying the gene from reverted to prototrophy at a high frequency. In these derivatives the gene became functional due to DNA rearrangements upstream of the coding sequence. Two types of rearrangement were characterized. One was identified as an insertion of IS2. The second was a deletion that resulted in transcription of the gene from the Tc gene promoter and translation from a newly created ribosome-binding site formed at the junction between the and vector DNA sequences.


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