1887

Abstract

The RecE protein of , known to be required for induction of the SOS response and of ϕ105 prophage, was shown to be involved in mitomycin C induction of diploid lysogens carrying a silent ϕ105 prophage in their unexpressed chromosome. These stable non-complementing diploid lysogens, formed by protoplast fusion and regeneration, did not synthesize repressor, so that the induction observed must have resulted from RecE-dependent activation of the prophage rather than from RecE-dependent inactivation of repressor. Mitomycin C treatment does not induce permanent expression of the silent chromosome, so the activation seems to be temporary, perhaps reflecting the action of an SOS function under RecE control.

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1986-06-01
2021-07-24
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