1887

Abstract

The adherence of LGV440(L1) to human HeLa 229 and mouse McCoy cells was stimulated by the lectin wheat germ agglutinin (WGA) and inhibited by the sugars -acetyl-D-glucosamine, -acetyl-D-galactosamine and chitobiose, but only when the chlamydiae had been passaged several times in HeLa cells. After passage in McCoy cells, the lectin and the sugars elicited little response. The non-LGV serovar UW-31(K), however, differed from LGV440(L1) in that, regardless of passage, the lectin and sugar effects were observed only in HeLa cells. Affinity chromatography on WGA-agarose confirmed that HeLa-grown LGV-440(L1) bound to a significantly greater extent relative to McCoy-grown chlamydiae. In addition, participation of heterogeneous chlamydial ligands was suggested by the observation that the adherence of heated (60°C, 5 min) UW-31(K) to HeLa cells at 37°C was not inhibited at all, but at 5°C, the adherence rate was greatly reduced, indicating the participation of heat-stable as well as heat-labile ligands. These data are interpreted to indicate that the passage history of results in the acquisition of altered surface components that participate in the initial interaction of the bacterium with the host.

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1986-06-01
2022-01-19
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References

  1. Allan I., Pearce J. H. 1979a; Host modification of chlamydiae: differential infectivity for cell monolayers of chlamydiae grown in eggs and monolayers.. Journal of General Microbiology 112:53–59
    [Google Scholar]
  2. Allan I., Pearce J. H. 1979b; Host modification of chlamydiae: presence of an egg antigen on the surface of chlamydiae grown in the chick embryo.. Journal of General Microbiology 112:61–66
    [Google Scholar]
  3. Bose S. K., Paul R. G. 1982; Purification of Chlamydia trachomatis lymphogranuloma venereum elementary bodies and their interaction with HeLa cells.. Journal of General Microbiology 128:1371–1379
    [Google Scholar]
  4. Bose S. K., Smith G. B. 1984; Positive co- operativity in the adherence between elementary bodies of Chlamydia trachomatis strain UW-31 and HeLa cells.. FEMS Microbiology Letters 23:55–58
    [Google Scholar]
  5. Bose S. K., Smith G. B., Paul R. G. 1983; Influence of lectins, hexoses, and neuraminidase on the association of purified elementary bodies of Chlamydia trachomatis UW-31 with HeLa cells.. Infection and Immunity 40:1060–1067
    [Google Scholar]
  6. Byrne G. I. 1978; Kinetics of phagocytosis of Chlamydia psittaci by mouse fibroblasts (L cells): separation of the attachment and ingestion stages.. Infection and Immunity 19:607–612
    [Google Scholar]
  7. Caldwell H. D., Perry L. J. 1982; Neutralization of Chlamydia trachomatis infectivity with antibodies to the major outer membrane protein.. Infection and Immunity 38:745–754
    [Google Scholar]
  8. Collier L. H. 1962; Growth characteristics of inclusion blenorrhea virus in cell cultures.. Annals of the New York Academy of Sciences 98:42–49
    [Google Scholar]
  9. Gordon F. B., Nichols R. L., Quan A. L. 1971; Immunotyping of Chlamydia trachomatis with fluorescent antibody: retention of immunospecificity in cell culture passage, and typing with infected cell monolayers.. in Trachoma and Related Disorders PP 358– 362 Editor Nichols R. L. Amsterdam: Excerpta Medica.;
    [Google Scholar]
  10. Griffiths M. S., Ainsworth S., Pearce J. H. 1976; Infectivity titration of guinea-pig inclusion conjunctivitis agent in irradiated McCoy cells.. Journal of General Microbiology 95:249–256
    [Google Scholar]
  11. Jenkin H. M. 1966; The continuous passage of agents of trachoma in cell culture. I. Characteristics of TW-3 and BOUR strains of trachoma cultivated in serial passage in HeLa 229 cells. . Journal of Infectious Diseases 116:390–399
    [Google Scholar]
  12. Kordova N., Poffenroth L., Wilt J. C. 1972a; Lysosomes and the ‘toxicity’ of Rickettsiales. II.Non-cytocidal interactions of egg-grown C. psittaci 6BC and in vitro macrophages.. Canadian Journal of Microbiology 18:869–873
    [Google Scholar]
  13. Kordova N., Poffenroth L., Wilt J. C. 1972b; Lysosomes and the ‘toxicity’ of Rickettsiales. III. Response of L cells infected with egg-attenuated C. psittaci 6BC strain.. Canadian Journal of Microbiology 18:1343–1348
    [Google Scholar]
  14. Kordova N., Martin C., Wilt J. C., Neuman M. 1977; Sucrose density differences of Chlamydia psittaci 6BC in relation to its host.. Canadian Journal of Microbiology 23:649–652
    [Google Scholar]
  15. Kramer R. H., Canellakis E. S. 1979; The surface glycoproteins of the HeLa cell. Internalization of wheat germ agglutinin-receptors.. Biochimica et biophysica acta 551:328–348
    [Google Scholar]
  16. Lee C. K. 1981; Interaction between a trachoma strain of Chlamydia trachomatis and mouse fibroblasts (McCoy cells) in the absence of centrifugation.. Infection and Immunity 31:584–591
    [Google Scholar]
  17. Levy N.J. 1979; Wheat germ agglutinin blockage of chlamydial attachment sites: antagonism by N- acetyl-D-glucosamine.. Infection and Immunity 25:946–953
    [Google Scholar]
  18. Mitsui Y., Kitamuro T., Fujimoto M. 1967; Adaptation of TRIC agents to tissue culture and characteristics of tissue culture adapted variants.. American Journal of Ophthalmology 63:1191–1205
    [Google Scholar]
  19. Neuman M. R., Kordova N., Wilt J. C. 1980; Host influence on the density of chlamydiae in Renografin gradients.. Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene (Abteilung I, Originale A) 247:526–529
    [Google Scholar]
  20. Peeling R., McLean I. W., Brunham R. C. 1984; In vitro neutralization of Chlamydia trachomatis with monoclonal antibody to an epitope on the outer membrane protein.. Infection and Immunity 46:484–88
    [Google Scholar]
  21. Schachter J., Grossman M., Holt J., Sweet R., Goodier E., Mille J. 1979; Prospective study of chlamydia infection in neonates.. Lancet ii,:377–380
    [Google Scholar]
  22. Soderlund G., Kihlstrom E. 1983; Attachment and internalization of a Chlamydia trachomatis lymphogranuloma venereum strain by McCoy cells: kinetics of infectivity and effect of lectins and carbohydrates.. Infection and Immunity 42:930–935
    [Google Scholar]
  23. Yamane I., Matsuya Y., Jimbo K. 1968; An autoclavable powdered culture medium for mammalian cells. . Proceedings of the Society for Experimental Biology and Medicine. 127:335–336
    [Google Scholar]
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