1887

Abstract

The protein content of EDTA extracts from 76 strains of Gram-positive anaerobic cocci was examined using SDS-PAGE. Strains of produced almost identical profiles; greater heterogeneity was observed within the species and , but several strains within each biotype produced similar patterns. Serological investigation of these extracts by ELISA revealed numerous cross-reactions among the different biotypes. Immunoblot transfers from polyacrylamide gels demonstrated two common antigens within strains of the species , but these were not species-specific.

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1986-06-01
2024-04-25
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References

  1. Busch D. F. 1984; Anaerobes in infections of the head and neck and ear, nose and throat.. Review of Infectious Diseases 6Supplement 1:115122
    [Google Scholar]
  2. CATO E. P., HASH D. E., HOLDEMAN L. V., MOORE W. E. C. 1982; Electrophoretic study of Clostridium species. . Journal of Clinical Microbiology 15:688702
    [Google Scholar]
  3. Cato E. P., Johnson J. L., Hash D. E., Holdeman L. V. 1983; Synonymy of Peptococcus glycinophilus (Cardon and Barker 1946) Douglas 1957 with Peptostreptococcus micros (Prevot 1933) Smith 1957 and electrophoretic differentiation of Peptostreptococcus micros from Peptococcus magnus (Prevot 1933)Holdeman and Moore 1972. International Journal of Systematic Bacteriology 33:207210
    [Google Scholar]
  4. Collee J. G., Watt B., Fowler E. B., Brown R. 1972; An evaluation of the Gaspak system in the culture of anaerobic bacteria. . Journal of Applied Bacteriology 35:7182
    [Google Scholar]
  5. Cousland G., Poxton I. R. 1983; Analysis of lipopolysaccharides of Bacteroides fragilis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and electroblot transfer. . FEMS Microbiology Letters 20:461465
    [Google Scholar]
  6. Deacon A. G., Duerden B. I., Holbrook W. P. 1978; Gas-liquid chromatographic analysis of metabolic products in the identification of Bacteroi- daceae of clinical interest. . Journal of Medical Microbiology 11:8189
    [Google Scholar]
  7. DOWELL V. R., HAWKINS T. M. 1974; Laboratory methods in bacteriology. CDC Laboratory Manual. . Department of Health Education and Welfare publication no. (CDC) 748272
    [Google Scholar]
  8. Engvall E., Perlmann P. 1972; Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labelled anti-immuno- globulin in antigen-coated tubes. . Journal of Immunology 109:129135
    [Google Scholar]
  9. GORBACH S. L., BARTLETT J. G. 1974; Anaerobic infections. . New England Journal of Medicine 290:1177,1237,1289–1294,1184,1245
    [Google Scholar]
  10. Graham M. B., Falkler W. A. 1979; Extractable- antigen shared by Peptostreptococcus anaerobius strains. . Journal of Clinical Microbiology 9:507510
    [Google Scholar]
  11. HOLDEMAN L. V., CATO E. P., MOORE W. E. C. 1977; Anaerobe Laboratory Manual, 4th edn. . Blacksburg, Virginia:: Virginia Polytechnic Institute and State University.;
    [Google Scholar]
  12. Ingham H. R., Selkon J. B., Roxby C. M. 1977; Bacteriological study of otogenic cerebral abscesses: chemotherapeutic role of metronidazole. . British Medical Journal 2:991993
    [Google Scholar]
  13. Jackman P. J. H. 1982; Classification of Corynebac- terium species from axillary skin by numerical analysis of electrophoretic protein patterns. . Journal of Medical Microbiology 15:485492
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. . Nature 227: London :680685
    [Google Scholar]
  15. Markowitz A., Lerner A. M. 1977; Differentiation of several isolates of Peptococcus magnus by counter-immunoelectrophoresis. . Infection and Immunity 16:152154
    [Google Scholar]
  16. Myhre E. B. 1984; Surface receptors for human serum albumin in Peptococcus magnus strains. . Journal of Medical Microbiology 18:189195
    [Google Scholar]
  17. Oakley B. R., Kirsch D. R., Morris N. R. 1980; A simplified ultra-sensitive silver stain for detecting proteins in polyacrylamide gels. . Analytical Biochemistry 105:361363
    [Google Scholar]
  18. POXTON I. R. 1979; Serological identification of Bacteroides species by an enzyme-linked immunosorbent assay. . Journal of Clinical Pathology 32:294298
    [Google Scholar]
  19. Poxton I. R., Brown R. 1979; Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of cell- surface proteins as an aid to identification of the Bacteroides fragilis group. . Journal of General Microbiology 112:211217
    [Google Scholar]
  20. Poxton I. R., Byrne M. D. 1981; Immunological analysis of the EDT A-soluble antigens of Clostridium difficile and related species. . Journal of General Microbiology 122:4146
    [Google Scholar]
  21. Poxton I. R., Sutherland I. W. 1976; The butanol-soluble proteins of Klebsiella aerogenes. . Microbios 15:93103
    [Google Scholar]
  22. Razim S., Rottem S. 1967; Identification of Mycoplasma and other microorganisms by polyacrylamide gel electrophoresis of cell proteins.. Journal of Bacteriology 94:1807–1810
    [Google Scholar]
  23. Stone M. L. 1940; Studies on the anaerobic streptococcus. I. Certain biochemical and immunological properties of anaerobic streptococci. . Journal of Bacteriology 39:559582
    [Google Scholar]
  24. Sutter V. L., Vargo V. L., Finegold S. M. 1975; Wadsworth Anaerobic Bacteriology Manual, 2nd edn. . Wadsworth Hospital Center, Nev., USA:: Anaerobic Bacteriology Laboratory;
    [Google Scholar]
  25. Thadepalli H., Gorbach S. L., Keith L. 1973; Anaerobic infections of the female genital tract: bacteriologic and therapeutic aspects. . American Journal of Obstetrics and Gynecology 117:1034–1040
    [Google Scholar]
  26. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. . Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  27. Watt B. 1972; The recovery of clinically important anaerobes on solid media. . Journal of Medical Microbiology 5:211–218
    [Google Scholar]
  28. Watt B., Jack E. P. 1977; What are anaerobic cocci? . Journal of Medical Microbiology 10:461–468
    [Google Scholar]
  29. Watt B., Bushell A. C., Wallace E. T. 1984; Characterisation of anaerobic cocci in the diagnostic laboratory. . Journal of Clinical Pathology 37:1197
    [Google Scholar]
  30. Wong M., Catena A., Hadley W. K. 1980; Antigenic relationships and rapid identification of Peptostreptococcus species. . Journal of Clinical Microbiology 11:515–521
    [Google Scholar]
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