1887

Abstract

Incubating cells of with sodium lauroyl sarcosinate, under conditions that did not cause lysis, solubilized material comprising 5-8% of the cell dry weight. The treatment reduced cell hydrophobicity, and reduced the ability of the cells to coaggregate with spp. The extract contained about 20 polypeptides and these were identified as being cell-surface components on the basis of one or more of the following criteria: () being degraded when cells were incubated with protease; () being labelled when cells were iodinated using a lactoperoxidase-catalysed reaction; () reacting with antibodies raised to fixed whole cells. Eight of the polypeptides accounted for more than 70% of the total protein extracted, and one component (molecular mass 16 kDa) was hydrophobic. The cell-surface proteins described are implicated in cell hydrophobicity and coaggregation.

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1986-06-01
2022-01-17
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References

  1. Appelbaum B., Rosan B. 1984; Cell surface proteins of oral streptococci.. Infection and Immunity 46:245–250
    [Google Scholar]
  2. Archibald A. R. 1972; Teichoic acids. . Methods in Carbohydrate Chemistry 6:162–172
    [Google Scholar]
  3. Beachey E. H., Ofek I. 1976; Epithelial cell binding of group A streptococci by lipoteichoic acid on fimbriae denuded of M protein. . Journal of Experimental Medicine 143:759–771
    [Google Scholar]
  4. Carlsson J. 1972; Nutritional requirements of Streptococcus sanguis. . Archives of Oral Biology 17:1327–1332
    [Google Scholar]
  5. Chen P. S., Jr Toribara T. Y., Warner H. 1956; Microdetermination of phosphorus. . Analytical Chemistry 28:1756–1758
    [Google Scholar]
  6. Chiu T. H., Emdur L. I., Platt D. 1974; Lipoteichoic acids from Streptococcus sanguis. . Journal of Bacteriology 118:471–479
    [Google Scholar]
  7. Cisar J. O., Kolenbrander P. E., McIntire F. C. 1979; Specificity of coaggregation reactions between human oral streptococci and strains of Actinomyces viscosus or Actinomyces naeslundii. . Infection and Immunity 24:742–752
    [Google Scholar]
  8. Cole R. M., Calandra G. B., Huff E., Nugent K. M. 1976; Attributes of potential utility in differentiating among "group H' streptococci or Streptococcus sanguis. . Journal of Dental Research 55:A142–A153
    [Google Scholar]
  9. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. . Analytical Chemistry 28:350–354
    [Google Scholar]
  10. Eifert R., Rosan B., Golub E. 1984; Optimization of an hydroxyapatite adhesion assay for Streptococcus sanguis. . Infection and Immunity 44:287–291
    [Google Scholar]
  11. Ellen R. P., Gibbons R. J. 1972; M protein- associated adherence of Streptococcus pyogenes to epithelial surfaces: prerequisite for virulence. . Infection and Immunity 5:826–830
    [Google Scholar]
  12. Emdur L. I., Saralkar C., McHugh J. G., Chiu T. H. 1974; Glycerolphosphate-containingcell wall polysaccharides from Streptococcus sanguis. . Journal of Bacteriology 120:724–734
    [Google Scholar]
  13. Fachon-Kalweit S., Elder B. L., Fives-Taylor P. 1985; Antibodies that bind to fimbriae block adhesion of Streptococcus sanguis to saliva-coated hydroxyapatite.. Infection and Immunity 48:617–624
    [Google Scholar]
  14. Fairbanks G., Steck T. L., Wallach D. F. H. 1971; Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. . Biochemistry 10:2606–2617
    [Google Scholar]
  15. Fives-Taylor P. M., Thompson D. W. 1985; Surface properties of Streptococcus sanguis FW213 mutants nonadherent to saliva-coated hydroxyapatite. . Infection and Immunity 47:752–759
    [Google Scholar]
  16. Forester H., Hunter N., Knox K. W. 1983; Characteristics of a high molecular weight extracellular protein of Streptococcus mutans. . Journal of General Microbiology 129:2779–2788
    [Google Scholar]
  17. Gibbons R. J., Etherden I. 1983; Comparative hydrophobicities of oral bacteria and their adherence to salivary pellicles. . Infection and Immunity 41:1190–1196
    [Google Scholar]
  18. Gibbons R. J., Nygaard M. 1970; Interbacterial aggregation of plaque bacteria. . Archives of Oral Biology 15:1397–1400
    [Google Scholar]
  19. Gibbons R. J., Van Houte J. 1975; Bacterial adherence in oral microbial ecology. . Annual Review oj Microbiology 29:19–44
    [Google Scholar]
  20. Gibbons R. J., Etherden I., Skobe Z. 1983; Association of fimbriae with the hydrophobicity of Streptococcus sanguis FC-1 and adherence to salivary pellicles. . Infection and Immunity 41:414–417
    [Google Scholar]
  21. Greenwood F. C., Hunter W. M., Glover J. S. 1963; The preparation of 1311 labelled human growth hormone of high specific radioactivity. . Biochemical Journal 89:114–123
    [Google Scholar]
  22. Hamada S., Slade H. D. 1980; Biology, immunology and cariogenicity of Streptococcus mutans. . Microbiological Reviews 44:331–384
    [Google Scholar]
  23. Hancock K., Tsang V. 1983; India ink staining of proteins on nitrocellulose paper. . Analytical Biochemistry 133:157–162
    [Google Scholar]
  24. Handley P. S., Carter P. L., Wyatt J. E., Hesketh L. M. 1985; Surface structures (peri- trichous fibrils and tufts of fibrils) found on Streptococcus sanguis strains may be related to their ability to coaggregate with other oral genera. . Infection and Immunity 47:217–227
    [Google Scholar]
  25. Hansen S. A. 1975; Thin-layer chromatographic method for the identification of mono-, di- and trisaccharides. . Journal of Chromatography 107:224–227
    [Google Scholar]
  26. Hardie J. M., Bowden G. H. 1976; Physiological classification of oral viridans streptococci. . Journal of Dental Research 55:A166–A176
    [Google Scholar]
  27. van Houte J., Gibbons R. J., Banghart S. B. 1970; Adherence as a determinant of the presence of Streptococcus salivarius and Streptococcus sanguis on the human tooth surface. . Archives of Oral Biology 15:1025–1034
    [Google Scholar]
  28. JENKINSON H. F., SAWYER W. D., MANDELSTAM J. 1981; Synthesis and order of assembly of spore coat proteins in Bacillus subtilis. . Journal of General Microbiology 123:1\2–16
    [Google Scholar]
  29. Kessler R. E., Shockman G. D. 1979; Precursor- product relationship of intracellular and extracellular lipoteichoic acids of Streptococcus faecium. . Journal of Bacteriology 137:869–877
    [Google Scholar]
  30. Knox K. W., Hardy L. N., Markevics L. J., Evans J. D., Wicken A. J. 1985; Comparative studies on the effect of growth conditions on adhesion, hydrophobicity and extracellular protein profile of Streptococcus sanguis G9B. . Infection and Immunity 50:545–554
    [Google Scholar]
  31. Kolenbrander P. E., Williams B. L. 1981; Lactose-reversible coaggregation between oral actin- omycetes and Streptococcus sanguis. . Infection and Immunity 33:95–102
    [Google Scholar]
  32. Laemmli U. K., Favre M. 1973; Maturation of the head of bacteriophage T4. I. DNA packaging events. . Journal of Molecular Biology 80:575–599
    [Google Scholar]
  33. Liljemark W. F., Bloomquist C. G. 1981; Isolation of a protein-containing cell surface component from Streptococcus sanguis which affects its adherence to saliva-coated hydroxyapatite. . Infection and Immunity 34:428–434
    [Google Scholar]
  34. McBride B. C., Morris E. J., Ganeshkumar N. 1985; Relationship of streptococcal cell surface proteins to hydrophobicity and adherence. . In Molecular Basis of Oral Adhesion pp. 85–93 editor Mergenhagen S. E., Rosan B. . Washington DC:: American Society for Microbiology;
    [Google Scholar]
  35. Mann A. F., Hucklesby D. P., Hewitt E. J. 1979; A modified Hanes and Isherwood spray for improved detection of phosphate esters in thin layer chromatography. . Analytical Biochemistry 96:6
    [Google Scholar]
  36. Marchalonis J. J. 1969; An enzymic method for the trace iodination of immunoglobulins and other proteins. . Biochemical Journal 113:299–305
    [Google Scholar]
  37. Merril C. R., Goldman D., Sedmen S. A., Ebert M. H. 1981; Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. . SciencelW1437–1438
    [Google Scholar]
  38. Murray P. A., Levine M. J., Tabak L. A., Reddy M. S. 1982; Specificity of salivary-bacterial interactions. II. Evidence for a lectin on Streptococcus sanguis with specificity for a NeuAca23Gal/Jl, 3GlcNac sequence. . Biochemical and Biophysical Research Communications 106:390–396
    [Google Scholar]
  39. Nesbitt W. E., Staat R. H., Rosan B., Taylor K. G., Doyle R. J. 1980; Association of protein with the cell wall of Streptococcus mutans. . Infection and Immunity 28:118–126
    [Google Scholar]
  40. Nesbitt W. E., Doyle R. J., Taylor K. G. 1982a; Hydrophobic interactions and the adherence of Streptococcus sanguis to hydroxylapatite. . Infection and Immunity 38:637–644
    [Google Scholar]
  41. Nesbitt W. E., Doyle R. J., Taylor K. G., Staat R. H., Arnold R. R. 1982A; Positive cooperati- vity in the binding of Streptococcus sanguis to hydroxylapatite. . Infection and Immunity 35:157–165
    [Google Scholar]
  42. Nugent K. M., Cole R. M. 1977; Characterization of group H streptococcal temperate bacteriophage 227. Journal of Virology 21:1061–1073
    [Google Scholar]
  43. Ogier J. A., Klein J. P., Sommer P., Frank R. M. 1984; Identification and preliminary characterization of saliva-interacting surface antigens of Streptococcus mutans by immunoblotting, ligand blotting and immunoprecipitation. . Infection and Immunity 45:107–112
    [Google Scholar]
  44. Rosan B. 1973; Antigens of Streptococcus sanguis. . Infection and Immunity 7:205–211
    [Google Scholar]
  45. Rosan B. 1978; Absence of glycerol teichoic acid in certain oral streptococci. . Science 201:918–920
    [Google Scholar]
  46. Rosan B., Appelbaum B. 1982; Surface receptors of selected oral streptococci and their role in adhesion to hydroxyapatite.. In Microbiology - 1982 pp. 342–345 editor Schlessinger D. Washington, DC :: American Society for Microbiology;
    [Google Scholar]
  47. Rosenberg W., Gutnick D., Rosenberg E. 1980; Adherence of bacteria to hydrocarbons: a simple method for measuring cell-surface hydrophobicity. FEMS Microbiology Letters 9:29–33
    [Google Scholar]
  48. Rosenberg M., Judes H., Weiss E. 1983; Cell surface hydrophobicity of dental plaque microorganisms in situ. . Infection and Immunity 42:831–834
    [Google Scholar]
  49. Russell R. R. B. 1979; Wall-associated protein antigens of Streptococcus mutans. . Journal of General Microbiology 114:109–115
    [Google Scholar]
  50. Sato S., Koga T., Inoue M. 1984; A possible mechanism for the cellular coaggregation between Actinomyces viscosus ATCC 19246 and Streptococcus sanguis ATCC 10557J. ournal of General Microbiology 130:1351–1357
    [Google Scholar]
  51. Scholler M., Klein J. P., Sommer P., Frank R. 1982; Common antigens of streptococcal and non- streptococcal oral bacteria : isolation and biochemical characterization of the extracellular protein antigen. . Journal of General Microbiology 128:2113–2120
    [Google Scholar]
  52. Smith R., Lehner T., Beverley P. C. L. 1984; Characterization of monoclonal antibodies to Streptococcus mutans antigenic determinants I/II,I,II and III and their serotype specificities. . Infection and Immunity 46:168–175
    [Google Scholar]
  53. Swanson J., King G., Zeligs B. 1975; Studies on gonococcus infection. VIII. 125Iodine labelling of gonococci and studies on their in vitro interactions with eukaryotic cells. . Infection and Immunity 11:453–459
    [Google Scholar]
  54. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. . Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  55. Wadstrom T., Schmidt K.-H., Kuhnemund O., Havlicek J., Kohler W. 1984; Comparative studies on surface hydrophobicity of streptococcal strains of groups A, B, C, D and G. . Journal of General Microbiology 130:657–664
    [Google Scholar]
  56. Weerkamp A. H., Jacobs T. 1982; Cell wall- associated protein antigens of Streptococcus salivarius: purification, properties and function in adherence. . Infection and Immunity 38:233–242
    [Google Scholar]
  57. Weerkamp A. H., McBride B. C. 1981; Identification of a Streptococcus salivarius cell wall component mediating coaggregation with Veillonella al- calescens VI. . Infection and Immunity 32:723–730
    [Google Scholar]
  58. Wicken A. J., KNOX K. W. 1975; Lipoteichoic acids: a new class of bacterial antigen. . Science 187:1161–1167
    [Google Scholar]
  59. Wicken A., Gibbens J. W., Knox K. W. 1973; Comparative studies on the isolation of membrane lipoteichoic acid from Lactobacillus ferment i. . Journal of Bacteriology 113:365–372
    [Google Scholar]
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