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Abstract
The structures of the mycolic acids from the cell wall skeleton of Rhodococcus lentifragmentus AN-115 were established. Field desorption mass spectrometry of underivatized mycolic acids showed that the molecular species of the mycolic acids were distributed from C38 to C50 and the major components were C44, C46 and C48 mono- and dienoic ?-hydroxy fatty acids. Pyrolysis gas chromatography-mass spectrometry confirmed that the mycolic acids consisted of a saturated straight C12 and C14 ?-chain and an unsaturated ?-chain, having mainly C29 and C31 carbons with one or two double bonds. The double bonds in the ?-chain were established as the cis isomers by nuclear magnetic resonance. The absolute configurations of the ?- and ?-asymmetric carbons were both established as R using molecular rotation data.
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