1887

Abstract

A streptomycin-resistant variant of was grown in defined nutrient-deficient media in both batch and continuous culture. The production, composition and viscosity of the extracellular polysaccharide (xanthan) synthesized by this strain were influenced by the fermentation time and nutrient exhaustion in batch culture and by the dilution rate in continuous culture. The specific rate of exopolysaccharide synthesis was maximal during exponential growth in all of the nutrient-deficient media studied although some xanthan was formed during stationary phase. The concentration of exopolysaccharide decreased at later stages of stationary phase in some cultures. Both the extent of acylation and the consistency index of xanthan isolates were low or minimal during exponential growth, maximal in polysaccharide isolated as the growth rate fell and usually lower after this time. Between dilution rates of 0·03 and 0·06 h in chemostat culture, the cell and exopolysaccharide dry weights were independent of dilution rate, the specific rate of xanthan synthesis decreasing at lower growth rates. Although the variation in the acyl content and consistency index was less than that observed in batch culture, exopolysaccharide isolated at higher dilution rates tended to have a higher acetyl content, lower pyruvyl content and lower consistency index.

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1986-06-01
2021-07-24
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References

  1. Blumenkrantz N., Asboe-Hansen G. 1973; New method for quantitative determination of uronic acids. . Analytical Biochemistry 54:484–489
    [Google Scholar]
  2. Cadmus M. C., Knutson C. A., Lagoda A. A., Pittsley J. E., Burton K. A. 1978; Synthetic media for production of quality xanthan gum in 20 liter fermentors.. Biotechnology and Bioengineering 20:1003–1014
    [Google Scholar]
  3. Cadmus M. C., Burton K. A., Slodki M. E. 1982; Growth-related substituent changes in exopolysaccharides of fast-growing Rhizobia. . Applied and Environmental Microbiology 44:242–245
    [Google Scholar]
  4. Charles M , Radjai M. K. 1977; Xanthan gum from acid whey. . Extracellular Microbial Polysaccharides, American Chemical Society Symposium In 45: pp 27–39 editor Sandford P. A., Laskin A. Washington: DC: American Chemical Society;
    [Google Scholar]
  5. Davidson I. W. 1978; Production of polysaccharide by Xanthomonas campestris in continuous culture. . FEMS Microbiology Letters 3:347–349
    [Google Scholar]
  6. Deavin L., Jarman T. R., Lawson C. J., Righelato R. C., Slocombe S. 1977; The production of alginic acid by Azotobacter vinelandii in batch and continuous culture. . In Extracellular Microbial Polysaccharides, American Chemical Society Symposium 45 pp. 14–26 editor Sandford P. A., Laskin A. Washington: DC: American Chemical Society;
    [Google Scholar]
  7. De Hollander J. A., Bettenhaussen C. W., Stouthamer A. H. 1979; Growth yields, polysaccharide production, and energy conservation in chemostat cultures of Rhizobium trifolii. . Antonie van Leeuwenhoek 45:401–405
    [Google Scholar]
  8. Dekker R. F. H., Candy G. P. 1979; The /J- mannanases elaborated by the phytopathogen Xanthomonas campestris. . Archives of Microbiology 122:297–299
    [Google Scholar]
  9. Dudman W. F. 1960; Cellulose production by Acetobacter strains in submerged culture. . Journal of General Microbiology 22:25–39
    [Google Scholar]
  10. Duguid J. P., Wilkinson J. F. 1953; The influence of cultural conditions on polysaccharide production by Aerobacter aerogenes. . Journal of General Microbiology 9:174–189
    [Google Scholar]
  11. Evans C. G.T., Yeo R. G., Ellwood D. C. 1979; Continuous culture studies on the production of extracellular polysaccharides by Xanthomonas jug- landis. . In Microbial Polysaccharides and Polysacchar- ases pp 51–68 editor Berkeley R. C. W. , Gooday G.W., Ellwood D. C. London: New York & San Francisco: Academic Press;
    [Google Scholar]
  12. Hestrin S. 1949; The reaction of acetylcholine and other carboxylic acid derivatives with hydroxyl- amine, and its analytical application. . Journal of Biological Chemistry 180:249–261
    [Google Scholar]
  13. Hisamatsu M., Sano K., Amemura A., Harada T. 1978; Acidic polysaccharides containing succinic acid in various strains of Agrobacterium. . Carbohydrate Research 61:89–96
    [Google Scholar]
  14. Holzwarth G., Ogletree J. 1979; Pyruvate-free xanthan. . Carbohydrate Research 76:277–280
    [Google Scholar]
  15. Horan N. J., Jarman T. R., Dawes E. A. 1981; Effects of carbon source and inorganic phosphate concentration on the production of alginic acid by a mutant of Azotobacter vinelandii and on the enzymes involved in its biosynthesis. . Journal of General Microbiology 127:185–191
    [Google Scholar]
  16. Jansson P.-E., Kenne L., Lindberg B. 1975; Structure of the extracellular polysaccharide from Xanthomonas campestris. . Carbohydrate Research 45:275–282
    [Google Scholar]
  17. Melton L. D., Mindt L., Rees D. A., Sanderson G. R. 1976; Covalent structure of the extracellular polysaccharide from Xanthomonas campestris: evidence from partial hydrolysis studies. . Carbohydrate Research 46:245–257
    [Google Scholar]
  18. Mian F. A., Jarman T. R., Righelato R. C. 1978; Biosynthesis of exopolysaccharide by Pseudomonas aeruginosa. . Journal of Bacteriology 134:418–422
    [Google Scholar]
  19. Nasuno S., Starr M. P. 1967; Polygalacturonic acid transeliminase of Xanthomonas campestris. . Biochemical Journal 104:178–185
    [Google Scholar]
  20. Piggott N. H., Sutherland I. W., Jarman T. R. 1982; Alginate synthesis by mucoid strains of Pseudomonas aeruginosa. . European Journal of Applied Microbiology 16:131–135
    [Google Scholar]
  21. SlLMAN R. W., Rogovin P. 1972; Continuous fermentation to produce xanthan biopolymer: effect of dilution rate. . Biotechnology and Bioengineering 14:23–31
    [Google Scholar]
  22. Slodki M. E., Cadmus M. C. 1978; Production of microbial polysaccharides. . Advances in Applied Microbiology 23:19–54
    [Google Scholar]
  23. Smith I. H., Symes K. C., Lawson C. J., Morris E. R. 1981; Influence of the pyruvate content of xanthan on macromolecular association in solution.. International Journal of Biological Macromolecules 3:129–134
    [Google Scholar]
  24. Sutherland I. W. 1981; Xanthomonas polysac- charides-improved methods for their comparison. . Carbohydrate Polymers 1:107–115
    [Google Scholar]
  25. Sutherland I. W. 1982; Biosynthesis of microbial exopolysaccharides. . Advances in Microbial Physiology 23:80–142
    [Google Scholar]
  26. Sutherland I. W. 1984; Hydrolysis of unordered xanthan in solution by fungal cellulases. . Carbohydrate Research 131:93–104
    [Google Scholar]
  27. Sutherland I. W., Ellwood D. C. 1979; Microbial exopolysaccharides - industrial polymers of current and future potential. . Symposia of the Society for General Microbiology 29:107–150
    [Google Scholar]
  28. Tait M. I. 1984; Exopolysaccharide production by Xanthomonas campestris. . PhD thesis UK: Edinburgh University;
    [Google Scholar]
  29. Wernau W. C. 1978; Microbiological Production of Xanthan Gums. . British Patent 2 008 600 A.
    [Google Scholar]
  30. Whitfield C., Sutherland I. W., Cripps R. E. 1981; Surface polysaccharides in mutants of Xanthomonas campestris. . Journal of General Microbiology 124:385–392
    [Google Scholar]
  31. Williams A. G., Wimpenny J. W. T. 1977; Exopolysaccharide production by Pseudomonas NCIB11264 grown in batch culture. . Journal of General Microbiology 102:13–21
    [Google Scholar]
  32. Williams A. G., Wimpenny J. W. T. 1978; Exopolysaccharide production by Pseudomonas NCIB11264 grown in continuous culture. . Journal of General Microbiology 104:47–57
    [Google Scholar]
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