1887

Abstract

Cadmium-adapted exhibited a long lag phase (6 h) on incubation in a defined medium containing 3 mM-Cd. During this time extensive blebbing of the outer membrane was observed by electron microscopy and polyphosphate granules containing Cd were present in the cells. Cells from exponential-phase cultures of cadmium-adapted were found in clusters. They were much smaller than control cells grown without cadmium, and contained electron-dense aggregates. Cadmium-adapted cells released more lipopolysaccharide and protein into the external medium than did control cells; the addition of Ca, but not Mg, to the medium prevented this increased release. Cadmium-adapted cells also showed greatly increased sensitivity towards certain antibiotics, including the aminoglycosides, cyclic polypeptides and doxycycline. It is suggested that this is related to changes in membrane structure.

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1986-06-01
2021-07-24
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References

  1. Burdett I. D. J., Murray R. G. E. 1974; Electron microscope study of septum formation in Escherichia coli strains B and B/R during synchronous growth. . Journal of Bacteriology 119:1039–1056
    [Google Scholar]
  2. Eagon R. G., Carson M. J. 1965; Lysis of cell walls and intact cells of Pseudomonas aeruginosa by ethylenediaminetetraacetic acid and by lysozyme. . Canadian Journal of Microbiology 11:193–201
    [Google Scholar]
  3. Hancock R. E. W. 1984; Alterations in outer membrane permeability. . Annual Review of Microbiology 38:237–264
    [Google Scholar]
  4. Herbert D., PHIPPS P. J., Strange R. E. 1971; Chemical analysis of microbial cells.. Methods in Microbiology 5B:209–343
    [Google Scholar]
  5. Higham D. P., Sadler P. J., Scawen M. D. 1984; Cadmium resistant Pseudomonas putida synthesise novel cadmium proteins. . Science 225:1043–1046
    [Google Scholar]
  6. Higham D. P., Sadler P. J., Scawen M. D. 1985; Cadmium resistance in Pseudomonas putida: growth and uptake of cadmium. . Journal of General Microbiology 131:2539–2544
    [Google Scholar]
  7. Irvin R. T., Chatterjee A. K., Sanderson K. E., Costerton J. W. 1975; Comparison of the cell envelope structure of a lipopolysaccharide defective (heptose-deficient) strain and a smooth strain of Salmonella typhimurium. . Journal of Bacteriology 124:930–941
    [Google Scholar]
  8. Laddaga R. A., Bessen R., Silver S. 1985; Cadmium resistance mutant of Bacillus subtilis 168 with reduced cadmium transport. . Journal of Bacteriology 162:1106–1110
    [Google Scholar]
  9. Leive L. 1974; The barrier function of the Gram- negative cell envelope. . Annals of the New York Academy of Sciences 235:109–129
    [Google Scholar]
  10. Meadow P. M. 1975; Wall and membrane structures in the genus Pseudomonas.. In Genetics and Biochemistry of Pseudomonas pp 67–98 editor Clarke P. H. , Richmond M. H. London: John Wiley;
    [Google Scholar]
  11. Morrison D. C., Leive L. 1975; Reactions of lipopolysaccaride from Escherichia coli 0111: B4 prepared by two extraction procedures. . Journal of Biological Chemistry 350:2911–2919
    [Google Scholar]
  12. Nikaido H., Nakae T. 1979; The outer-membrane of Gram-negative bacteria. . Advances in Microbial Physiology 20:163–250
    [Google Scholar]
  13. Norris J. R., Swain H. 1971; Staining bacteria. . Methods in Microbiology 5A:105–134
    [Google Scholar]
  14. Pawlett D. 1983; Cadmium resistance in pseudomonads. . PhD thesis University of London;
    [Google Scholar]
  15. Reynolds T. C. 1963; The use of lead citrate at high pH as an electron opaque stain in electron microscopy. . Journal of Cell Biology 17:208–212
    [Google Scholar]
  16. Roantree R. J., Kuo T., Macphee D. G., Stocker B. A. D. 1969; Effect of various rough lesions in Salmonella typhimurium upon sensitivity to antibiotics. . Bacteriological Proceedings, Abstract M76 p.79
    [Google Scholar]
  17. Rosenthal K. S., Swanson P. E., Storm D. R. 1976; Disruption of Escherichia coli outer membranes by EM49 a new membrane active peptide antibiotic. . Biochemistry 15:5783–5792
    [Google Scholar]
  18. Sanderson K. E., MacAlister T., Costerton T. W. 1974; Permeability of lipopolysaccharide deficient (rough) mutants of Salmonella typhimurium to antibiotics, lysozyme and other agents. . Canadian Journal of Microbiology 20:1135–1145
    [Google Scholar]
  19. Schindler M., Osborn M. J. 1979; Interactions of divalent cations and polymyxin B with lipopolysaccharide. . Biochemistry 18:4425–4430
    [Google Scholar]
  20. Schindler P. R. G., Teuber M. 1975; Action of polymyxin B on bacterial membranes: morphological changes in the cytoplasm and in the outer membrane of Salmonella typhimurium and Escherichia coli B. . Antimicrobial Agents and Chemotherapy 8:95–104
    [Google Scholar]
  21. Storm D. R., Rosenthal K. S., Swanson P. E. 1977; Polymyxin and related polypeptide antibiotics. . Annual Review of Biochemistry 46:723–763
    [Google Scholar]
  22. Tynecka Z., Gos Z., Zajac J. 1981; Energy dependent efflux of cadmium coded by a plasmid resistance determinant in Staphylococcus aureus. . Journal of Bacteriology 147:313–319
    [Google Scholar]
  23. Vaara M., Vaara T. 1983; Sensitization of Gram- negative bacteria to antibiotics and complement by a non-toxic oligopeptide. . Nature London : 303:526–528
    [Google Scholar]
  24. Weissbach A., Hurwitz j. 1959; The formation of 2-keto-3-deoxyhepturonic acid in extracts of Escherichia coli. I. Identification. . Journal of Biological Chemistry 234:705–709
    [Google Scholar]
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