Estimation of the Mr of Isocitrate Lyase Messenger RNA from Chlorella fusca

Desmond S. T. Nicholl; Peter C. L. John

doi:10.1099/00221287-132-5-1423

Volume 132, Issue 5

Research Article

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Estimation of the M r of Isocitrate Lyase Messenger RNA from Chlorella fusca

Desmond S. T. Nicholl^1,† and Peter C. L. John^1,‡
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Affiliations: ¹ Botany Department, The Queen's University of Belfast, Belfast BT7 INN, UK

† Present address: Department of Developmental Biology, Research School of Biological Sciences, The Australian National University, PO Box 475, Canberra City, ACT 2601, Australia.

‡ Present address: Biology Department, Paisley College of Technology, High Street, Paisley PA1 2BE, UK.
Published: 01 May 1986 https://doi.org/10.1099/00221287-132-5-1423

Abstract

The mRNA for the adaptive enzyme isocitrate lyase (ICL) from Chlorella fusca has been identified by fractionation of total poly(A)-containing RNA and in vitro translation followed by immune precipitation. The M r of ICL mRNA was approximately 8·0 x 105, which is in good agreement with a previous estimate obtained by in vivo double-labelling.