%0 Journal Article %A García, Pedro %A García, Ernesto %A Ronda, Concepción %A Lopez, Rubens %A Jiang, R. Z. %A Tomasz, Alexander %T Mutants of Streptococcus pneumoniae That Contain a Temperature-sensitive Autolysin %D 1986 %J Microbiology, %V 132 %N 5 %P 1401-1405 %@ 1465-2080 %R https://doi.org/10.1099/00221287-132-5-1401 %I Microbiology Society, %X Two mutants of Streptococcus pneumoniae deficient in autolysin activity produced a protein that showed immunological identity with the N-acetyl-muramyl-l-alanyl-amidase present in the wild-type strain, when tested with antiserum obtained against this enzyme. The protein was produced by the mutant cultures grown either at 37 °;C or at 30 °C, although only the cell extracts obtained at 30 °;C showed significant cell wall hydrolysing activity. In contrast to the lysis resistance of these bacteria grown at 37 °;C, mutant cultures grown at 30 °;C exhibited significant degrees of autolysis when treated with detergent or cell wall inhibitors. Extracts of the mutant cultures contained a cell wall hydrolysing activity that was rapidly inactivated during incubation at 37 °;C. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-132-5-1401