Iron Regulated Outer Membrane Proteins of Escherichia coli: Variations in Expression Due to the Chelator Used to Restrict the Availability of Iron

Henrik Chart; Martin Buck; Pauline Stevenson; Elwyn Griffiths

doi:10.1099/00221287-132-5-1373

Volume 132, Issue 5

Research Article

Free

Iron Regulated Outer Membrane Proteins of Escherichia coli: Variations in Expression Due to the Chelator Used to Restrict the Availability of Iron

Henrik Chart¹, Martin Buck^2,†, Pauline Stevenson¹ and Elwyn Griffiths¹
View Affiliations Hide Affiliations

Affiliations: ¹ National Institute for Biological Standards and Control, Holly Hill, Hampstead, London NW3 6RB, UK ² Department of Biochemistry, University of California, Berkeley, California 94720, USA

† Present address: Agricultural and Food Research Council, Unit of Nitrogen Fixation, The University of Sussex, Brighton BN1 9RQ, UK.
Published: 01 May 1986 https://doi.org/10.1099/00221287-132-5-1373

Abstract

Iron restriction was induced in Escherichia coli O111, E. coli O164 and E. coli C by growing the organisms in trypticase soy broth containing ovotransferrin, desferal, EDDA (ethylenediamine-dihydroxyphenylacetic acid) or α,α'-dipyridyl. There were marked qualitative and quantitative differences in the iron regulated outer membrane proteins expressed in the presence of the various iron chelators. Differences in the kinetics of growth were also noted. E. coli C was devoid of a ferric enterobactin iron uptake system.