1887

Abstract

accumulated glycogen and trehalose in a characteristic way during growth on solid medium. Glycogen storage in the substrate mycelium took place during development of the aerial mycelium. The concentration of nitrogen source in the culture medium influenced the time at which accumulation started as well as the maximum levels of polysaccharide stored. Degradation of these glycogen reserves was observed near the beginning of sporulation. The onset of sporogenesis was always accompanied by a new accumulation of glycogen in sporulating hyphae. During spore maturation the accumulated polysaccharide was degraded. No glycogen was observed in aerial non-sporulating hyphae or in mature spores. Trehalose was detected during all phases of colony development. A preferential accumulation was found in aerial hyphae and spores, where it reached levels up to 12% of the cell dry weight. The possible roles of both carbohydrates in the developmental cycle of are discussed.

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1986-05-01
2021-07-28
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References

  1. BARTON J.K., DENHOLLANDER J.A., HOPFIELD J.J., SHULMAN R.G. 1982; 13C nuclear magnetic resonance study of trehalose mobilization in yeast spores.. Journal of Bacteriology 151:177–185
    [Google Scholar]
  2. BERGERE J.L., ROUSSEAU M., , MERCIER C. 1975; Polyoside intracellulaire impliqut dam la sporulation de Clostridium butyricum. Annales de microbio-Iogie 126A:295–314
    [Google Scholar]
  3. BRANA A.F., MANZANL M.B., HARDISSON C. 1980; Occurrence of polysaccharide granules in sporulating hyphae of Streptomyces viridochromogenes.. Journal of Bacteriology 144:1139–1142
    [Google Scholar]
  4. BRANA A.F., MANZANAL M.B., HARDISSON C. 1982; Characterization of intracellular polysaccharides of Streptomyces.. Canadian Journal oJ'Microbiology 28:1320–1323
    [Google Scholar]
  5. COLEMAN R.H., ENSIGN J.C. 1982; Regulation of formation of aerial mycelia and spores of Streptomyces viridochromogenes. Journal ofBactei'iology 149:1102–1111
    [Google Scholar]
  6. CROWE L.W., MOURADIAN R., , CROWE J.H., JACKSON S.A., WOMERSLEY C. 1984; Effects of carbohydrates on membrane stability at low water activities. Biochimica et biophysica acta 769:141–150
    [Google Scholar]
  7. DAWES E.A., SENIOR P.J. 1973; Energy reserve polymers in micro-organisms.. Advances in Microbial Phisiology 10:136–266
    [Google Scholar]
  8. ELBEIN A.D. 1967; Carbohydrate metabolism in streptomycetesII. Isolation and enzymatic synthesis of trehalose. Journal of’ Bacteriology: 94:1520–1524
    [Google Scholar]
  9. ENSIGN J.C. 1982; Developmental biology of actinomycetes. In Overproduction of Microbial Products,. London pp. 127–140 Krumphanzl, V., Sikyta Z., Vanek. Z. New York:: Academic Press.;
    [Google Scholar]
  10. , HARDISSO C., MANZANAL M.B. 1976; U ltrastructural studies of sporulation in Streptomyces. Journal of’ Bacteriology 127:1443–1454
    [Google Scholar]
  11. HARDISSON C., MANZANL M.B., SALAS J.A., SUAREZ J.E. 1978; Fine structure, physiology and biochemistry of arthrospore germination in Streptomyces antibioticus.. Journal of’ General Microbiology 105:203–214
    [Google Scholar]
  12. HEY-FERGUSON A, MITCHELL M., ELBEIN A.D. 1973; Trehalose metabolism in germinating spores of Streptomyces hygroscopicus. Journal ofBacteriology 116:1084–1085
    [Google Scholar]
  13. KALAKOUTSKII L.V., AGRE N.S. 1976; 1976).C omparative aspects of development and differentiation in actinomycetes.. Bacteriological Reviews 40:469–524
    [Google Scholar]
  14. KANE S.M. 1974; Carbohydrate metabolism during ascospore development in yeast.. Journal of Bacteriology 118:8–14
    [Google Scholar]
  15. LLOYD J.B., WHELAN W.J. 1969; 1969).A n improved method for enzymic determination of glucose in the presence of maltose.. Analytical Biochemistry 30:467–470
    [Google Scholar]
  16. MENDEZ C., BARBES C., MANZANAL M.B., HARDISSON C. 1985a; A simple method to study colony development in Streptomyces.. FEMS Microbiology Letters 28:99–102
    [Google Scholar]
  17. MENDEZ C., BRANA A.F., MANZANAL M.B., HARDISSON C. 1985b; Role of substrate mycelium in colony development in Streptomyces. Canadian Journal of’ Microbiology 31:446–450
    [Google Scholar]
  18. NIMI, O., AMANO Y., IKEDA A., YOSHIMURA Y., SUGIYAMA M., NOMI R. 1984; Intracellular accumulation of trehalose during streptomycin formation by Streptomyces griseus.. Archives of Biochemistry and Biophysics Chemistry 48:285–290
    [Google Scholar]
  19. ROSEN H. 1957; A modified ninhydrin colorimetric analysis for amino acids.. Archives of Biochemistry and Biophysics 67:10–15
    [Google Scholar]
  20. RYTER A, KELLENBERGER E. 1958; Etude au microscope electronique de plasma contenant de I’acide desoxyribonucleique.. Zeitschrft fur Natur-Jorschung 13b:597–605
    [Google Scholar]
  21. STRASDINE G.A. 1972; The role of intracellular glucan in endogenous fermentation and spore maturation in Clostridium botulinum type E.. Canadian Journal of’ Microbiology 18:211–217
    [Google Scholar]
  22. THEVELEIN J.M. 1984; Regulation of trehalose mobilization in fungi.. Microbiological Reviews 48:42–59
    [Google Scholar]
  23. THIERY J.P. 1967; M ise en evidence des polysaccharides sur coupes fines en microscopie electronique.. Journal de Microscopie 6:987–1018
    [Google Scholar]
  24. TREVELYAN W.E., E.,P ROCTER D.P., HARRISON J.S. 1950; Detection of sugars on paper chromatograms.. Nature, London: 166:444–445
    [Google Scholar]
  25. WILDERMUTH H. 1970; 1970). Development and organization of the aerial mycelium in Streptomyces coelicolor.. Journal of’ General Microbiology 60:43–50
    [Google Scholar]
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