@article{mbs:/content/journal/micro/10.1099/00221287-132-5-1215, author = "Thurnheer, Thomas and Köuhler, Thilo and Cook, Alasdair M. and Leisinger, Thomas", title = "Orthanilic Acid and Analogues as Carbon Sources for Bacteria: Growth Physiology and Enzymic Desulphonation", journal= "Microbiology", year = "1986", volume = "132", number = "5", pages = "1215-1220", doi = "https://doi.org/10.1099/00221287-132-5-1215", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-132-5-1215", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Carbon-limited aerobic batch enrichment cultures were grown and 17 bacteria able to degrade orthanilic acid (2-aminobenzenesulphonic acid), sulphanilic acid, sulphonamide, 4-sulphobenzoic acid, and benzene-, toluene- and phenolsulphonic acids were isolated. The organisms could each use one to three of the substances. Strain O-1, a Pseudomonas sp., which utilized three of these compounds, was studied in detail. A complete mass balance was obtained for the growth of the organism in medium containing, for example, orthanilic acid, and a specific growth rate of 0·1 h-1 was observed. Cell extracts desulphonated six aromatic sulphonates. The enzyme(s) was soluble and was not synthesized in succinate-grown cells. Enzyme activity [about 40 μkat (kg protein)-1] was dependent on the presence of catalytic amounts of NAD(P)H.", }