1887

Abstract

The effect of the β-lactam antibiotic cephalexin on the spiral conformation of cells of spp. was examined by scanning electron microscopy. and , which are known to have a left-handed spiral shape, elongated and still showed left-handed spirals in medium containing cephalexin. The spiral conformation of the elongated cells is therefore considered to represent the natural condition. The spiral conformations of and grown in ordinary cultures were difficult to determine because they have short cells without a complete spiral. After cephalexin treatment, the cells of these species elongated and displayed spiral forms, right-handed in and left-handed in . This elongation method may be useful for checking and determination of the spiral handedness of short spiral or curved bacteria such as vibrios.

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/content/journal/micro/10.1099/00221287-132-4-877
1986-04-01
2021-07-27
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References

  1. Carleton O., Charon N. W., Allender P., O’Brien S. 1979; Helix handedness of Leptospira interrogans as determined by scanning electron microscopy.. Journal of Bacteriology 137:1413–1416
    [Google Scholar]
  2. Krieg N. R. 1976; Biology of the chemoheterotro- phic spirilla.. Bacteriological Reviews 40:55–115
    [Google Scholar]
  3. Krieg N. R. 1984; Aerobic/microaerophilic, motile, helical/vibrioid Gram-negative bacteria.. In Bergey's Manual of Systematic Bacteriology vol.1 pp 7–1124 Editor Krieg N. R., Krieg N. R. Baltimore: Williams & Wilkins.;
    [Google Scholar]
  4. Krieg N. R., Smibert R. M. 1974; Spiral and curved bacteria.. In Bergey’s Manual of Determinative Bacteriology 8th edn: pp 196–216 Editor Buchanan R. E., Gibbons N. E. Baltimore: Williams & Wilkins;
    [Google Scholar]
  5. Mendelson N. H. 1978; Helical Bacillus subtilis macrofibers: morphogenesis of a multicellular microorganism.. Proceedings of the National Academy of Sciences of the United States of America 75:2478–2482
    [Google Scholar]
  6. Ryter A., Kellenberger E. 1958; L’inclusion au polyester pour l’ultramicrotome.. Journal of Ultrastructure Research 2:200–214
    [Google Scholar]
  7. Terasaki Y. 1972; Studies in the genus Spirillum Ehrenberg. I. Morphological, physiological, and biochemical characteristics of water spirilla.. Bulletin of Suzugamine Women's College 161–146
    [Google Scholar]
  8. Yoshii Z. 1978; Fundamental considerations and experimental conditions on the study of the spiral- direction of helical bacteria.. Bulletin of Yamaguchi Medical School 25:115–127
    [Google Scholar]
  9. Yoshii Z., Konishi H., Akitomi H., Mizuno A. 1982; Spirality of cell bodies in genus Spirillum and genus Rhodospirillum under scanning electron microscopy.. Bulletin of Yamaguchi Medical School 29:1–8
    [Google Scholar]
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