%0 Journal Article %A Nugent, Marilyn E. %A Smith, Terence J. %A Tacon, William C. A. %T Characterization and Incompatibility Properties of ROM- Derivatives of pBR322-based Plasmids %D 1986 %J Microbiology, %V 132 %N 4 %P 1021-1026 %@ 1465-2080 %R https://doi.org/10.1099/00221287-132-4-1021 %I Microbiology Society, %X We have characterized a copy number mutant of the pBR322-based plasmid pWT111. A single nucleotide transversion in loop II’ of RNAI results in an eightfold increase in plasmid copy number. Removal of the rom coding region from pWT111cop results in a further sixfold increase in copy number. We present evidence that ROM is involved in the strong incompatibility effect seen between pMB1 and ColE1 type plasmids. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-132-4-1021