@article{mbs:/content/journal/micro/10.1099/00221287-132-4-1021, author = "Nugent, Marilyn E. and Smith, Terence J. and Tacon, William C. A.", title = "Characterization and Incompatibility Properties of ROM- Derivatives of pBR322-based Plasmids", journal= "Microbiology", year = "1986", volume = "132", number = "4", pages = "1021-1026", doi = "https://doi.org/10.1099/00221287-132-4-1021", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-132-4-1021", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "We have characterized a copy number mutant of the pBR322-based plasmid pWT111. A single nucleotide transversion in loop II’ of RNAI results in an eightfold increase in plasmid copy number. Removal of the rom coding region from pWT111cop results in a further sixfold increase in copy number. We present evidence that ROM is involved in the strong incompatibility effect seen between pMB1 and ColE1 type plasmids.", }