1887

Abstract

We have characterized a copy number mutant of the pBR322-based plasmid pWT111. A single nucleotide transversion in loop II’ of RNAI results in an eightfold increase in plasmid copy number. Removal of the coding region from pWT111cop results in a further sixfold increase in copy number. We present evidence that ROM is involved in the strong incompatibility effect seen between pMB1 and ColE1 type plasmids.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-4-1021
1986-04-01
2021-08-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/4/mic-132-4-1021.html?itemId=/content/journal/micro/10.1099/00221287-132-4-1021&mimeType=html&fmt=ahah

References

  1. BOLIVAR F., RODRIGUZ R.L., GREENE P.J., BETLACH M.C., HEYNECKER H.L., BOYER H.W., CROSA J.H., FALKOW S. 1977; Construction and characterization of new cloning vehicles. Gene 2:95–113
    [Google Scholar]
  2. CASADABAN M.J, CHOU J., COHEN S.N. 1980; In citro gene fusions that join an enzymatically active bgalactosidase segment to amino-terminal fragments of exogenous proteins.. Journal of' Bacteriology 143:971–980
    [Google Scholar]
  3. CESARENI G., MUESING M.A., POLISKY B. 1982; Control of ColEl DNA replication: the rop gene product negatively affects transcription from the replication primer promoter.. Proceedings of the National Academy of' Sciences of' the United States of' America 79:6313–6317
    [Google Scholar]
  4. DAVISON J. 1984; Mechanism of control of DNA replication and incompatibility in ColE 1 -type plasmids- a review.. Gene 28:1–15
    [Google Scholar]
  5. HASHIMOTO-GOTOH T., TIMMIS K.N. 1981; Incompatibility properties of ColE1 and pMBl derivative plasmids: random replication of multicopy replicons.. Cell 23:229–238
    [Google Scholar]
  6. HERSHFIELD V., BOYER H.W., CHOW L., HELINSKI D.R. 1976; Characterization of a mini-ColEl plasmid.. Journal OJ Bacteriology 126:447–453
    [Google Scholar]
  7. KADO C.I., LIU S.T. 1981; Rapid procedure for detection and isolation of large and small plasmids.. Journal of Bacteriology 145:1365–1373
    [Google Scholar]
  8. MAXAM A.M., GILBERT W. 1977; A new method for sequencing DNA.. Proceedings of' the National Academy of' Sciences of the United States qj' America 74:560–564
    [Google Scholar]
  9. SUTCLIFFE J.G. 1979; Complete nucleotide sequence of the Escherichia coli plasmid pBR322.. Cold Spring Harbor Symposia on Quantitatire Biology 43:77–99
    [Google Scholar]
  10. TACON W.C.A, CAREY N., EMTAGE S. 1980; The construction and characterization of plasmid vectors suitable for the expression of all DNA phases under the control of the E. coli tryptophan promoter.. Molecular and General Genetics 177:427–428
    [Google Scholar]
  11. TOMIZAWA J.I., ITOH T. 1981; Plasmid ColEI incompatibility determined by interaction of RNAI with primer transcript.. Proceedings of' the Nationul Academy of' Sciences oj' the United States y j ' America 78:6096–6100
    [Google Scholar]
  12. TOMIZAWA J.I., SOM T. 1984; Control of ColEl plasmid replication: enhancement of binding of RNAI to the primer transcript by the Rom protein.. Cell 38:871–878
    [Google Scholar]
  13. TOMIZAWA J.I., ITOH T., SELZER G., SOM T. 1981; Inhibition of ColEl RNA primer formation by a plasmid-specified small RNA.. Proceedings of the National Academy of'Sciences ?/'the United Stairs oj' America 78:1421–1425
    [Google Scholar]
  14. TWIGG A.J., SHERRATT D. 1980; Trans-complementable copy-number mutants of plasmid ColE 1 .. Nature, Landon 283:216–218
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-4-1021
Loading
/content/journal/micro/10.1099/00221287-132-4-1021
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error