1887

Abstract

SUMMARY: A variety of biologically important pyridine nucleotides and precursors were examined for their capacities to satisfy the V-factor requirement of 30 strains of porcine haemophili. Of the compounds tested, only NAD, NMN and nicotinamide riboside (NR) supported the growth of all strains; NADP supported the growth of only the type strain of Further studies with the type strain and the neotype strain of demonstrated that, during growth, these organisms exhibited affinities for NMN that were greater than those for NAD; the affinity of for NR was similar to that for NMN, whereas exhibited relatively low affinity for NR. With either organism, equimolar amounts of NAD and NMN supported the production of approximately equal amounts of biomass whereas growth yields were substantially lower when NR was the pyridine nucleotide source. When either organism was grown in the presence of excess exogenous [-C]NAD, cessation of growth was accompanied by the apparent exhaustion of the NAD supply. Approximately 80% of the radioactivity added as [C]NAD could be recovered as extracellular [C]nicotinamide and the majority of the assimilated radioactive material was present intracellularly in the form of a [C]NAD(P) pool. The results are discussed in terms of the structural features required of a pyridine compound for it to support the growth of porcine haemophili, the capacity of these organisms to compete for pyridine nucleotide sources , and possible mechanisms involved in the assimilation of such compounds.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-3-807
1986-03-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/3/mic-132-3-807.html?itemId=/content/journal/micro/10.1099/00221287-132-3-807&mimeType=html&fmt=ahah

References

  1. Andersen K. B., von Meyenburg K. 1977; Charges of nicotinamide adenine nucleotides and adenylate energy charge as regulatory parameters of the metabolism in Escherichia coli. Journal of Biological Chemistry 252:4151–4156
    [Google Scholar]
  2. Artman M., Frankl G. 1982; Nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate splitting enzyme(s) of sheep and rabbit erythrocytes: their effect on the growth of Haemophilus. Canadian Journal of Microbiology 28:696–702
    [Google Scholar]
  3. Artman M., Seeley R. J. 1979; Nicotinamide adenine dinucleotide splitting enzyme: a plasma membrane protein of murine macrophages. Archives of Biochemistry and Biophysics 195:121–127
    [Google Scholar]
  4. Bachur N. R., Kaplan N. O. 1955; Metabolism of diphosphopyridine nucleotide and related compounds in Haemophilus parainfluenzae. Bacteriological Proceedings 55:116
    [Google Scholar]
  5. Bernofsky C. 1980a; Physiologic aspects of pyridine nucleotide regulation in mammals. Molecular and Cellular Biochemistry 33:135–143
    [Google Scholar]
  6. Bernofsky C. 1980b; Isolation and analysis of pyridine nucleotides and related compounds by liquid chromatography. Methods in Enzymology 66:23–39
    [Google Scholar]
  7. Bernofsky C., Pankow M. 1973; Protein binding of nicotinamide adenine dinucleotide and regulation of nicotinamide adenine dinucleotide glycohydro-lase activity in homogenates of rabbit skeletal muscle. Archives of Biochemistry and Biophysics 156:143–153
    [Google Scholar]
  8. Bernofsky C., Swan M. 1973; An improved cycling assay for nicotinamide adenine dinucleotide. Analytical Biochemistry 53:452–458
    [Google Scholar]
  9. Falconer D. F., Spector M. P., Foster J. W. 1984; Membrane association of NAD pyrophosphatase in Salmonella typhimurium. Current Microbiology 10:237–242
    [Google Scholar]
  10. Foster J. W., Baskowsky-Foster A. M. 1980; Pyridine nucleotide cycle of Salmonella typhimurium: in vivo recycling of nicotinamide adenine dinucleotide. Journal of Bacteriology 142:1032–1035
    [Google Scholar]
  11. Foster J. W., Moat A. G. 1980; Nicotinamide adenine dinucleotide biosynthesis and pyridine ucleotide cycle metabolism in microbial systems. Microbiological Reviews 44:83–105
    [Google Scholar]
  12. Foster J. W., Kinney D. M., Moat A. G. 1979; Pyridine nucleotide cycle of Salmonella typhimurium: isolation and characterization of pncA, pncB and pncC mutants and utilization of exogenous nicotinamide adenine dinucleotide. Journal of Bacteriology 137:1165–1175
    [Google Scholar]
  13. Gingrich W., Schlenk F. 1944; Codehydrogenase I and other pyridinium compounds as V-factor for Hemophilus influenzae and H. parainfluenzae. Journal of Bacteriology 47:535–550
    [Google Scholar]
  14. Goodman S. I., Wyatt R. J., Trepel J. B., Neckers L. M. 1982; NAD glycohydrolase: enzyme characterization using intact mammalian erythrocytes. Comparative Biochemistry and Physiology 71B:333–336
    [Google Scholar]
  15. Hillyard D., Rechsteiner M., Manlapaz-Ramos P., Imperial J. S., Cruz L. J., Olivera B. M. 1981; The pyridine nucleotide cycle. Studies in Escherichia coli and the human cell line D98/AH2. Journal of Biological Chemistry 256:8491–8497
    [Google Scholar]
  16. Johnson G. S. 1984; A pyrophosphatase which degrades NAD+ is located on the external surface of cultured fibroblasts: evidence that NAD+ is not extruded during treatment with N-methyl-N'-nitro-N-nitrosoguanidine. Archives of Biochemistry and Biophysics 229:538–543
    [Google Scholar]
  17. Kahn D. W., Anderson B. M. 1983; Studies of Haemophilus influenzae NAD pyrophosphatase. Biochemistry 22:8a
    [Google Scholar]
  18. Kilian M. 1976; A taxonomic study of the genus Haemophilus, with the proposal of a new species. Journal of General Microbiology 93:9–62
    [Google Scholar]
  19. Kilian M., Nicolet J., Biberstein E. L. 1978; Biochemical and serological characterization of Haemophilus pleuropneumoniae (Matthews & Patti-son 1961) Shope 1964 and proposal of a neotype strain. International Journal of Systematic Bacteriology 28:20–26
    [Google Scholar]
  20. Kothari R. M., Taylor M. W. 1982; Simultaneous separation of water-soluble vitamins and coenzymes by re versed-phase high-performance liquid chromatography. Journal of Chromatography 247:187–192
    [Google Scholar]
  21. Lamborg M., Stolzenbach F. E., Kaplan N. O. 1958; The nicotinic acid analogue of diphospho-pyridine nucleotide. Journal of Biological Chemistry 231:685–694
    [Google Scholar]
  22. Lilius E.-M., Multanen V.-M., Toivonen V. 1979; Quantitative extraction and estimation of intracellular nicotinamide nucleotides of Escherichia coli. Analytical Biochemistry 99:22–27
    [Google Scholar]
  23. Liu G., Foster J. W., Manlapaz-Ramos P., Olivera B. M. 1982; Nucleoside salvage pathway for NAD biosynthesis in Salmonella typhimurium. Journal of Bacteriology 152:1111–1116
    [Google Scholar]
  24. Lundquist R., Olivera B. M. 1971; Pyridine nucleotide metabolism in Escherichia coli. I. Exponential growth. Journal of Biological Chemistry 246:1107–1116
    [Google Scholar]
  25. Lundquist R., Olivera B. M. 1973; Pyridine nucleotide metabolism in Escherichia coli. II. Niacin starvation. , . Journal of Biological Chemistry 248:5137–5143
    [Google Scholar]
  26. McPheat W. L., Wardlaw A. C. 1980; Uptake of [14C]nicotinic acid and [14C]nicotinamide by Bordetella pertussis. FEMS Microbiology Letters 7:341–343
    [Google Scholar]
  27. Muller H. M., Muller C. D., Schuber F. 1983; NAD+ glycohydrolase, an ectoenzyme of calf spleen cells. Biochemical Journal 212:459–464
    [Google Scholar]
  28. Murakawa C., Takahashi T. 1978; Transport of nicotinamide adenine dinucleotide in an unc mutant of Escherichia coli. Journal of Biochemistry 84:1641–1643
    [Google Scholar]
  29. Nicolet J., Scholl E. 1981; Haemophilus infections. Edited by A. D. Leman, R. D. Glock, W. L. Mengeling, R. H. C. Penny, E. Scholl & B. Straw. Ames: Iowa State University Press. In Diseases of Swine 5th edn, pp:368–377
    [Google Scholar]
  30. Niven D. F., Collins P. A., Knowles C. J. 1977; Adenylate energy charge during batch culture of Beneckea natriegens. Journal of General Microbiology 98:95–108
    [Google Scholar]
  31. O’Reilly T., Rosendal S., Niven D. F. 1984; Porcine haemophili and actinobacilli: characterization by means of API test strips and possible taxonomic implications. Canadian Journal of Microbiology 30:1229–1238
    [Google Scholar]
  32. Pinder S., Clark J. B., Greenbaum A. L. 1971; The assay of intermediates and enzymes involved in the synthesis of the nicotinamide nucleotides in mammalian tissues. Methods in Enzymology 18B:20–46
    [Google Scholar]
  33. Pirt S. J. 1975; Principles of Microbe and Cell Cultivation. New York: Halsted Press. 4–14
    [Google Scholar]
  34. Schlenk F., Gingrich W. 1942; Nicotinamide-containing nutrilites for Hemophilus parainfluenzae. Journal of Biological Chemistry 143:295–296
    [Google Scholar]
  35. Sebunya T. N. K., Saunders J. R. 1983; Haemophilus pleuropneumoniae infection in swine: a review. Journal of the American Veterinary Medical Association 182:1331–1337
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-3-807
Loading
/content/journal/micro/10.1099/00221287-132-3-807
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error