Mechanism of Dodecane Uptake by Whole Cells of Free

Abstract

SUMMARY: Whole cells of took up -dodecane in two stages. The hydrocarbon was first passively adsorbed to the cell surface, and then hydrocarbon was taken in by a mechanism that obeyed Michaelis–Menten saturation kinetics [ 1 m, 12∙1 nmol min (mg protein)]. Under conditions of poor agitation the initial adsorption limited uptake rates. The organism accumulated unaltered substrate to higher concentrations within the cytosol than in the surrounding medium, but this depended upon high concentrations of dodecane being adsorbed to the cell surface, thus creating a diffusion gradient.

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1986-03-01
2024-03-29
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References

  1. Cooney J. J., Siporin C., Smucker R. A. 1980; Physiological and cytological responses to hydrocarbons by the hydrocarbon-using fungus Cladosporium resinae. Botanica marina 23:227–232
    [Google Scholar]
  2. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  3. Eisenthal R., Cornish-Bowden A. 1974; The direct linear plot: a new graphical procedure for estimating enzyme kinetic parameters. Biochemical Journal 139:715–720
    [Google Scholar]
  4. Goma G., Pareilleux A., Durand G. 1973; Specific hydrocarbon solubilization during growth of Candida lipolytica. Journal of Fermentation Technology 51:616–618
    [Google Scholar]
  5. Käppeli O., Fiechter A. 1976; The mode of interaction between the substrate and the cell surface of the hydrocarbon-utilizing yeast Candida tropicalis. Biotechnology and Bioengineering 18:967–974
    [Google Scholar]
  6. Käppeli O., Fiechter A. 1977; Component from the cell surface of the hydrocarbon-utilizing yeast Candida tropicalis with possible relation to hydrocarbon transport. Journal of Bacteriology 131:917–921
    [Google Scholar]
  7. Käppeli O., Fiechter A. 1981; Properties of hexadecane uptake by Candida tropicalis. Current Microbiology 6:21–26
    [Google Scholar]
  8. Lindley N. D., Heydeman M. T. 1983; Uptake of vapour phase [14C]dodecane by whole mycelia of Cladosporium resinae. Journal of General Microbiology 129:2301–2305
    [Google Scholar]
  9. Meisel M. N., Medvedeva G. A., Kozlova T. M., Domoshnikova N. A., Zaikina A. I., Fedoseeva G. E. 1973; Regularities of penetration into yeast cells of higher fatty acids and hydrocarbons, iheir intracellular migration and concentration. In Proceedings of the 3rd International Specialized Symposium on Yeasts, Helsinki/Otaniemi 2, pp. 149–168 Edited by H. Suomalainen & C. Waller. Helsinki: The Symposium, 1973.
    [Google Scholar]
  10. Neihof R. A., Klemme D. E., Patouillet C. E., Hannan P. J. 1981; Microbial contamination of ships fuels. In Distillate Fuel Stability and Cleanliness ASTM STP 751, pp. 126–135 Edited by L. L. Stavinoha & C. P. Henry. Philadelphia: American Society for Testing and Materials.
    [Google Scholar]
  11. Parberry D. G. 1971; Biological problems in jet aviation fuel and the biology of Amorphotheca resinae. Materials and Organisms 6:161–208
    [Google Scholar]
  12. park P. B. 1975; Biodeterioration in aircraft fuel systems. Technical Series of Society for Applied Bacteriology 9:105–126
    [Google Scholar]
  13. Rosenberg M., Gutnick D., Rosenberg E. 1980; Adherence of bacteria to hydrocarbons: a simple method for measuring cell-surface hydrophobicity. FEMS Microbiology Letters 9:29–33
    [Google Scholar]
  14. Smucker R. A., Cooney J. J. 1981; Cytological responses of Cladosporium resinae when shifted from glucose to hydrocarbon medium. Canadian Journal of Microbiology 27:1209–1218
    [Google Scholar]
  15. Turner A. P. F., Higgins I. J., Gull K. 1980; Microbodies in Cladosporium resinae grown on glucose and n-alkanes. FEMS Microbiology Letters 9:115–119
    [Google Scholar]
  16. Walker J. D., Cooney J. J. 1973; Pathway of n-alkane oxidation in Cladosporium resinae. Journal of Bacteriology 115:635–639
    [Google Scholar]
  17. Walker J. D., Cooney J. J. 1975; Effects of poorly metabolised hydrocarbons on substrate oxidation by Cladosporium resinae. Journal of Applied Bacteriology 39:189–195
    [Google Scholar]
  18. Wilkinson G. N. 1961; Statistical estimations in enzyme kinetics. Biochemical Journal 80:324–332
    [Google Scholar]
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