SUMMARY: Whole cells of took up -dodecane in two stages. The hydrocarbon was first passively adsorbed to the cell surface, and then hydrocarbon was taken in by a mechanism that obeyed Michaelis-Menten saturation kinetics [ 1 m, 12·1 nmol min (mg protein)]. Under conditions of poor agitation the initial adsorption limited uptake rates. The organism accumulated unaltered substrate to higher concentrations within the cytosol than in the surrounding medium, but this depended upon high concentrations of dodecane being adsorbed to the cell surface, thus creating a diffusion gradient.


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