1887

Abstract

SUMMARY: The nucleotide sequence of a 286 bp fragment containing the control region of has been determined. The transcriptional start of the gene has been located and the promoter signals identified. Various fragments of the promoter-proximal region were fused with the gene. Results obtained with these fusions indicate that the operator-promoter sites are located on a 110 bp restriction fragment. The determination of the amino acid sequence of the NH-terminus of the gene product revealed that the gene is not initiated with the AUG codon but with the unusual GTG codon. CRP, the cyclic AMP receptor protein, does not bind to the control region DNA even though expression of the gene is sensitive to catabolite repression.

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1986-03-01
2024-04-24
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References

  1. Aoyama T., Takanami M., Ohtsuka E., Taniyama Y., Marumoto R., Sato M., Ikehara M. 1983; Essential structure of E. coli promoter: effect of spacer length between the two consensus sequences on promoter function. Nucleic Acids Research 11:5855–5864
    [Google Scholar]
  2. Ashwell G. 1962; Enzymes of glucuronic and galacturonic acid metabolism in bacteria. Methods in Enzymology 5:190–208
    [Google Scholar]
  3. Bachmann B. J., Low K. B. 1980; Linkage map of Escherichia coli K-12. Microbiological Reviews 44:1–56
    [Google Scholar]
  4. Blanco C., Mata-Gilsinger M., Ritzenthaler P. 1983; Construction of hybrid plasmids containing the Escherichia coli uxaB gene: analysis of its regulation and direction of transcription. Journal of Bacteriology 153:747–755
    [Google Scholar]
  5. Boyer H. W., Roulland-Dussoix D. 1969; A complementation analysis of the restriction and modification of DNA in Escherichia coli. Journal of Molecular Biology 41:459–472
    [Google Scholar]
  6. Burke J. F. 1984; High sensitivity SI mapping with single-stranded [32P]DNA probes synthesized from bacteriophage M13mp templates. Gene 30:63–68
    [Google Scholar]
  7. Chang A. C. Y., Cohen S. N. 1978; Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. Journal of Bacteriology 134:1141–1156
    [Google Scholar]
  8. De Crombrugghe B., Busby S., Buc M. 1984; Cyclic AMP receptor protein: role in transcription activation. Science 224:831–838
    [Google Scholar]
  9. Dente L., Cesareni G., Cortese R. 1983; pEMBL: a new family of single-stranded plasmids. Nucleic Acids Research 11:1645–1655
    [Google Scholar]
  10. Dessein A., Schwartz M., Ullman A. 1978; Catabolite repression in Escherichia coli mutants lacking cyclic AMP. Molecular and General Genetics 162:83–87
    [Google Scholar]
  11. Fried M. G., Crothers D. M. 1983; CAP and RNA polymerase interactions with the lac promoter: binding stoichiometry and low-range effects. Nucleic Acids Research 11:141–158
    [Google Scholar]
  12. Garner M., Revzin A. 1981; Gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the E. coli lactose operon regulatory system. Nucleic Acids Research 9:3047–3060
    [Google Scholar]
  13. Gheysen D., Iserentant D., Derom C., Fiers W. 1982; Systematic alteration of the nucleotide sequence preceding the translation initiation codon and the effects on bacterial expression of the cloned SV40 small-T-antigen gene. Gene 17:55–63
    [Google Scholar]
  14. Hawley D. K., McClure W. R. 1983; Compilation and analysis of E. coli promoter DNA sequences. Nucleic Acids Research 11:2237–2255
    [Google Scholar]
  15. Hugouvieux-Cotte-Pattat N. 1981; Etude de la regulation de la voie de degradation des hexuronates chez E. coli K-12, par analyse de fusion de genes. Thesis, Institut National des Sciences Appliquees de Lyon.
    [Google Scholar]
  16. Kolb A., Spassky A., Chapon C., Blazy B., Buc H. 1983; On the different binding affinities of CRP at the lac, gal and malT promoter regions. Nucleic Acids Research 11:7833–7852
    [Google Scholar]
  17. Kornberg H., Watts P. D., Brown K. 1980; Mechanisms of‘inducer exclusion’ by glucose. FEBS Letters 117:K28–K36
    [Google Scholar]
  18. Kozak M. 1983; Comparison of initiation of protein synthesis in procaryotes, eucaryotes and organelles. Microbiological Reviews 47:1–45
    [Google Scholar]
  19. Maniatis T., Sambrook J. 1982; Cold Spring Harbor, NY: Cold Spring Harbor Laboratory. Molecular Cloning, a Laboratory Manual.
    [Google Scholar]
  20. Miller J. H. 1972; Cold Spring Harbor, NY: Cold Spring Harbor Laboratory. Experiments in Molecular Genetics.
    [Google Scholar]
  21. Minton N. P. 1984; Improved plasmid vectors for the isolation of translational lac gene fusions. Gene 31:269–273
    [Google Scholar]
  22. Portalier R., Stoeber F. 1972; La D-altronate: NAD-oxydoreductase d’E. coli K-12: purification, proprietes et individualite. European Journal of Biochemistry 26:50–61
    [Google Scholar]
  23. Portalier R., Robert-Baudouy J. , Stoeber F. . 1980; Regulation of Escherichia coli K-12 hexuron-ate system genes: exu regulon. Journal of Bacteriology 143:1095–1107
    [Google Scholar]
  24. Ritzenthaler P., Mata-Gilsinger M. 1982; Use of in vitro gene fusions to study the uxuR regulatory gene in Escherichia coli K-12: direction of transcription and regulation of its expression. Journal of Bacteriology 150:1040–1047
    [Google Scholar]
  25. Ritzenthaler P., Mata-Gilsinger M., Stoeber F. 1981; Molecular cloning of Escherichia coli K-12 hexuronate system genes: exu region. Journal of Bacteriology 145:181–190
    [Google Scholar]
  26. Ritzenthaler P., Blanco C., Mata-Gilsinger M. 1983; Interchangeability of repressors for the control of the uxu and uid operons in E. coli K-12. Molecular and General Genetics 191:263–270
    [Google Scholar]
  27. Robert-Baudouy J., Portalier R., Stoeber F. 1974; Regulation du metabolisme des hexuronates chez E. coli K-12: modalites de l’induction des enzymes du systeme hexuronate. European Journal of Biochemistry 43:1–15
    [Google Scholar]
  28. Rosenberg M., Court D. 1979; Regulatory sequences involved in the promotion and termination of RN A transcription. Annual Review of Genetics 13:319–353
    [Google Scholar]
  29. Salser W., Gestland R., Bolle A. 1967; In vitro synthesis of bacteriophage lysozyme. Nature, London 215:588–591
    [Google Scholar]
  30. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  31. Shine J., Dalgarno L. 1974; The 3' terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosomebinding sites. Proceedings of the National Academy of Sciences of the United States of America 71:1342–1346
    [Google Scholar]
  32. Steege D. A., Dalgarno L. 1974; 5' terminal nucleotide sequence of Escherichia coli lactose repressor mRNA: features of translational initiation and reinitiation sites. Proceedings of the National Academy of Sciences of the United States of America 74:4163–4167
    [Google Scholar]
  33. Stormo G., Schneider T., Gold L. 1982; Characterization of translational initiation sites in E. coli. Nucleic Acids Research 10:2971–2996
    [Google Scholar]
  34. Twigg A. J., Sherratt D. 1980; Trans-complementable copy number mutants of plasmid ColE1. Nature, London 10:2971–2996
    [Google Scholar]
  35. Ullman A., Danchin A. 1980; Role of cyclic AMP in regulatory mechanisms in bacteria. Trends in Biological Sciences 5:95–96
    [Google Scholar]
  36. Ullman A., Tillier F., Monod J. 1976; Catabolite modulator factor: a possible mediator of catabolite repression in bacteria. Proceedings of the National Academy of Sciences of the United States of America 73:3476–3479
    [Google Scholar]
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