1887

Abstract

SUMMARY: The nucleotide sequence of a 286 bp fragment containing the control region of has been determined. The transcriptional start of the gene has been located and the promoter signals identified. Various fragments of the promoter-proximal region were fused with the gene. Results obtained with these fusions indicate that the operator-promoter sites are located on a 110 bp restriction fragment. The determination of the amino acid sequence of the NH-terminus of the gene product revealed that the gene is not initiated with the AUG codon but with the unusual GTG codon. CRP, the cyclic AMP receptor protein, does not bind to the control region DNA even though expression of the gene is sensitive to catabolite repression.

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/content/journal/micro/10.1099/00221287-132-3-697
1986-03-01
2021-05-14
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