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Abstract
SUMMARY: We have transferred the hairy root plasmid (pRi) from Agrobacterium rhizogenes into Rhizobium trifolii. The plasmid fully expressed its root-inducing activity in the R. trifolii (pRi) transconjugant. Generally, the R. trifolii (pRi) transconjugant was unable to nodulate clover. However, occasionally a spontaneous mutant(s) of the R. trifolii (pRi) transconjugant arose which was capable of nodulating clover and of inducing root proliferation in a number of plant species. The mutant(s) induced significantly more nodules than the wild-type bacterium, but significantly less N2 was fixed by these nodules.
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© Society for General Microbiology 1986