1887

Abstract

SUMMARY: Twenty-five strains were characterized for lipopolysaccharide (LPS) profiles, outer membrane protein profiles, serum sensitivity, plasmid profiles and DNA homology. Seventeen strains produced low- LPS that did not contain O-sidechains, while the remaining eight strains contained ladder-like LPS suggestive of O-repeated units. This is the first time in the genus that LPS with O-repeated groups has been described. The strains producing the different types of LPS could not be distinguished from each other in outer membrane protein profiles or the other characteristics examined.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-3-611
1986-03-01
2021-05-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/3/mic-132-3-611.html?itemId=/content/journal/micro/10.1099/00221287-132-3-611&mimeType=html&fmt=ahah

References

  1. Albritton W. L. 1982; Infections due to Haemophi lus species other than H. influenzae. Annual Review of Microbiology 11:199–216
    [Google Scholar]
  2. Bruun B., Christensen J. J., Kilian M. 1984; Bacteremia caused by a beta-lactamase producing Haernophilus parainfuenzae strain of a new biotype. Acta pathologica, microbiologica et immunologica scandinavica B92:135–138
    [Google Scholar]
  3. Filip C., Fletcher B., Wulff J. L, Earhart C. F. 1973; Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. Journal of Bacteriology 115:717–722
    [Google Scholar]
  4. Gmeiner J., Schlecht S. 1979; Molecular organization of the outer membrane of Salmonella typhimurium LT2. European Journal of Biochemistry 93:609–620
    [Google Scholar]
  5. Goldman R.C., Lieve L. 1980; Heterogeneity of antigenic side chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella typhimurium LT2. European Journal of Biochemistry 107:145–153
    [Google Scholar]
  6. Hitchcock p.J., Brown T.M. 1983; Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. Journal of Bacteriology 154:269–277
    [Google Scholar]
  7. Inzana T.J. 1983; Electrophoretic heterogeneity and interstrain variation of the lipopolysaccharide of Haemophilus influenzae. Journal of Infectious Diseases 148:492–499
    [Google Scholar]
  8. Kilian M. 1976; A taxonomic study of the genus Haemophilus, with the proposal of a new species. Journal of General Microbiology 93:9–62
    [Google Scholar]
  9. Kilian M., Frederiksen W. 1980; Ecology of Haemophilus, Pasteurella and Actinobacillus. In Haemophilus, Pasteurella and Actinobacillus. Edited by M. Kilian, W. Frederiksen & E. L. Biberstein. New York: Academic Press.11–37
    [Google Scholar]
  10. Mietzner T.A.., Luginbuhl G.H., Sandstrom E., Morse S.A. 1984; Identification of an iron-regulated 37,000 dalton protein in the cell envelope of Neisseria gonorrhoeae. Infection and Immunity 45:410–416
    [Google Scholar]
  11. Mintz C. S., Apicella M. A., Morse S. A. 1984; Electrophoretic and serological characterization of the lipopolysaccharide produced by Neisseria gonorrhoeae. . Journal of Infectious Diseases 149:544–552
    [Google Scholar]
  12. Morse S. A., Mintz C. S., Sarafian S. K., Bartenstein L., Bertram M., Apicella M. A. 1983; Effect of dilution rate on lipopolysaccharide and serum resistance of Neisseria gonorrhoeae grown in continuous culture. Infection and Immunity 41:74–82
    [Google Scholar]
  13. Odumeru J. A., Ronald A. R., Albritton W. L. 1983; Characterization of cell proteins in Haemophilus ducreyi by polyacrylamide gel electrophoresis. Journal of Infectious Diseases 14:710–714
    [Google Scholar]
  14. Odumeru J. A., Wiseman G. M., Ronald A. R. 1984; Electrophoretic profiles of the lipopolysaccharide and the outer membrane proteins of virulent and avirulent Haemophilus ducreyi strains, In Program and Abstracts of the International Conjoint S.T.D. meeting June 17-21, 1984, Montreal, Canada. 48:17–21
    [Google Scholar]
  15. Pohl S. 1980; DNA relatedness among members of Haemophilus, Pasteurella and Actinobacillus. In Haemophilus, Pasteurella and Actinobacillus, Edited by M. Kilian, W. Frederiksen & E. L. Biberstein. New York: Academic Press. 245–253
    [Google Scholar]
  16. Roberts M, Swenson C. D., Owens L. M., Smith A. L. 1980; Characterization of chloramphenicol-resistant Haemophilus influenzae. . Antimicrobial Agents and Chemotherapy 18:610–615
    [Google Scholar]
  17. Taylor P. W. 1983; Bactericidal and bacteriolytic activity of serum against Gram-negative bacteria. Microbiological Reviews 47:46–83
    [Google Scholar]
  18. Tsai C.-M., Frasch C. E. 1982; A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Analytical Biochemistry 119:115–119
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-3-611
Loading
/content/journal/micro/10.1099/00221287-132-3-611
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error