1887

Abstract

Summary: The physico-chemical basis for the effects of nutrient conditions on the attachment of four freshwater isolates, sp. and sp., to hydrophobic (PD) and relatively hydrophilic (TCD) polystyrene surfaces was investigated. Different nutrient conditions and growth rates resulted in changes in the physico-chemistry of the bacterial surfaces, measured either by liquid contact angles on lawns of cells or by hydrophobic and electrostatic interaction chromatography of cells, and in different levels of attachment to the substrata. The phenotypic changes in cell surfaces and the levels of attachment were different for each species. Levels of bacterial adhesion differed for the two substrata, indicating different adhesion interactions with PD and TCD surfaces. Treatment of attached cells with chloramphenicol did not cause detachment of any of the bacteria from PD or TCD, whereas periodate and protease treatment removed some attached cells, the degree of detachment depending on the species. The presence of complex organic molecules, in the liquid phase and conditioning the solid surface, influenced the extent of bacterial attachment, the effect depending on the substratum, organic concentration and bacterial species. The results suggest that changes in nutrient conditions in natural aquatic habitats will affect the attachment of individual bacterial species differently, thus influencing the population structure of developing biofilms.

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1986-02-01
2024-12-12
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References

  1. Absolom D. R., Lamberti F. V., Policova Z., Zingg W., van Oss C. J., Neumann A. W. 1983; Surface thermodynamics of bacterial adhesion. Applied and Environmental Microbiology 46:90–97
    [Google Scholar]
  2. Baier R. E. 1980; Substrate influences on adhesion of microorganisms and their resultant new surface properties. In Adsorption of Microorganisms to Surfaces, pp. 59–104 Edited by G. Bitton & K. C. Marshall. New York: John Wiley & Sons.
    [Google Scholar]
  3. Baier R. E. 1981; Early events of micro-fouling of cell heat transfer equipment. In Fouling of Heat Transfer Equipment, pp. 293–304 Edited by E. F. C. Somerscales & J. G. Knudsen. Washington, DC: Hemisphere Publishing Co.
    [Google Scholar]
  4. Baker J. H. 1984; Factors affecting the bacterial colonization of various surfaces in a river. Canadian Journal of Microbiology 30:511–515
    [Google Scholar]
  5. Baszkin A., Lymann D. J. 1980; The interaction of plasma proteins with polymers. I. Relationship between polymer surface energy and protein adsorption and desorption. Journal of Biomedical Materials Research 14:393–403
    [Google Scholar]
  6. Corpe W. A. 1974a; Detachment of marine periphy-tic bacteria from surfaces of glass slides. Developments in Industrial Microbiology 15:281–287
    [Google Scholar]
  7. Corpe W. A. 1974b; Periphytic marine bacteria in the formation of microbial film on solid surfaces. In Effects of the Ocean Environments on Microbial Activities, pp. 397–417 Edited by R. Colwell & R. Morita. Baltimore: University Park Press.
    [Google Scholar]
  8. Dählback B. O., Hermansson M., Kjelleberg S., Norkrans B. 1981; The hydrophobicity of bacteria an important factor in their initial adhesion at the air-water interface. Archives of Microbiology 128:267–270
    [Google Scholar]
  9. Dexter S. C. 1977; Influence of substrate wettability on the formation of bacterial slime films on solid surfaces immersed in natural sea water. In Proceedings of the 4th International Congress on Marine Corrosion and Fouling, pp. 137–144 Edited by V. Romanovsky. Boulogne: Centre de Recherches et d’Etudes Oceanographiques.
    [Google Scholar]
  10. Eirich F. R. 1977; The conformational states of macromolecules adsorbed at solid liquid interfaces. Journal of Colloid and Interface Sciences 58:423–436
    [Google Scholar]
  11. Ellwood D. C., Tempest D. W. 1972; Effects of environment on bacterial wall content and composition. Advances in Microbial Physiology 7:43–47
    [Google Scholar]
  12. Ellwood D. C., Keevil E. C., Marsh P. D., Brown C. M., Wardell J. N. 1982; Surface associated growth. Philosophical Transactions of the Royal Society. Series B 297:517–532
    [Google Scholar]
  13. Feldner J., Bredt W., Kahne I. 1983; Influence of cell shape and surface charge on attachment of Mycoplasma pneumoniae to glass surfaces. Journal of Bacteriology 153:1–5
    [Google Scholar]
  14. Fletcher M. 1976; The effects of proteins on bacterial attachment to polystyrene. Journal of General Microbiology 94:400–404
    [Google Scholar]
  15. Fletcher M. 1977; The effects of culture concentration and age, time and temperature on bacterial attachment to polystyrene. Canadian Journal of Microbiology 23:1–6
    [Google Scholar]
  16. Fletcher M. 1980; Adherence of marine microorganisms to smooth surfaces. In Bacterial Adherence, pp. 345–374 Edited by E. H. Beachey. London: Chapman & Hall.
    [Google Scholar]
  17. Fletcher M., Loeb G. I. 1979; Influence of substratum characteristics on the attachment of a marine pseudomonad to solid surfaces. Applied and Environmental Microbiology 37:67–72
    [Google Scholar]
  18. Fletcher M., Marshall K. C. 1982; Bubble contact angle method for evaluating substratum interfacial characteristics and its relevance to bacterial attachment. Applied and Environmental Microbiology 44:184–192
    [Google Scholar]
  19. Fletcher M., Pringle J. H. 1985; The effect of surface free energy and medium surface tension on bacterial attachment to solid surfaces. Journal of Colloid and Interface Science 104:5–14
    [Google Scholar]
  20. Hogt A. H., Feijen J., Dankert J., de Vries J. A. 1982; Adhesion of Staphylococcus epidermidis and Staphylococcus saprophyticus onto FEP-teflon and cellulose acetate. In Biomedical Polymers. Proceedings of the Biological Engineering Society, pp. 39–47 London: The Biological Engineering Society.
    [Google Scholar]
  21. Horrocks D. L. 1977; The H Number Concept. Technical report 1095 NUC77-IT of the Scientific Instruments Division, Beckman Instruments.
    [Google Scholar]
  22. Jarman T. R., Deavin L., Slocombe S., Righelato R. C. 1978; Investigation of the effect of environmental conditions on the rate of exopolysaccharide synthesis in Azotobacter vinelandii. Journal of General Microbiology 107:59–64
    [Google Scholar]
  23. Kjelleberg S., Hermansson M. 1984; Starvation-induced effects on bacterial surface characteristics. Applied and Environmental Microbiology 84:497–503
    [Google Scholar]
  24. Lips A., Jessup N. E. 1979; Colloidal aspects of bacterial adhesion. In Adhesion of Microorganisms to Surfaces, pp. 3–27 Edited by D. C. Ellwood, S. Melling & P. Rutter. London: Academic Press.
    [Google Scholar]
  25. Loeb G. I., Neihof R. A. 1975; Marine conditioning films. In Applied Chemistry at Protein Interfaces. Advances in Chemistry Series 145, pp. 319–335 Edited by R. E. Baier. Washington, DC: Applied Chemistry Society.
    [Google Scholar]
  26. Marshall K. C. 1967; Electrophoretic properties of fast and slow growing species of Rhizobium. Australian Journal of Biological Sciences 20:429–438
    [Google Scholar]
  27. Marshall K. C. 1974; Mechanism of adhesion of marine bacteria to surfaces. In Proceedings of the 3rd International Congress on Marine Corrosion and Fouling, pp. 625–633 Edited by R. F. Acker, B. F. Brown, J. R. DePalma & W. P. Iverson. Evanston, Illinois: Northwestern University Press.
    [Google Scholar]
  28. Meadows P. S. 1971; The attachment of bacteria to solid surfaces. Archives of Microbiology 75:374–381
    [Google Scholar]
  29. Norde W., LyklemA J. 1978; Adsorption of proteins from aqueous solution on negatively charged polystyrene surfaces. In Ions of Macromolecular and Biological Systems. Proceedings of the 29th Symposium of Colston Research Society, pp. 11–33 Edited by D. H. Everett & B. Vincent. Bristol: Biotechnica.
    [Google Scholar]
  30. Paul J. H. 1984; Effects of antimetabolites on the adhesion of an estuarine Vibrio sp. to polystyrene. Applied and Environmental Microbiology 48:924–927
    [Google Scholar]
  31. Pederson K. 1980; Electrostatic interaction chromatography, a method for assaying the relative surface charges of bacteria. FEMS Microbiology Letters 12:365–367
    [Google Scholar]
  32. Pethica B. A. 1980; Microbial and cell adhesion. In Microbial Adhesion to Surfaces, pp. 19–45 Edited by R. C. W. Berkeley, J. M. Lynch, J. Melling, P. R. Rutter & B. Vincent. Chichester: Ellis Horwood.
    [Google Scholar]
  33. Plummer D. T., James A. M., Gooder H., Maxted W. R. 1962; Some physical investigations of the behaviour of bacterial surfaces. V. The variation of the surface structure of Streptococcus pyogenes during growth. Biochimica et biophysica acta 60:595–603
    [Google Scholar]
  34. Pringle J. H., Fletcher M. 1983; Influence of substratum wettability on attachment of freshwater bacteria to solid surfaces. Applied and Environmental Microbiology 45:811–817
    [Google Scholar]
  35. Pringle J. H., Fletcher M., Ellwood D. C. 1983; Selection of attachment mutants during the continuous culture of Pseudomonas fluorescens and relationship between attachment ability and surface composition. Journal of General Microbiology 129:2557–2569
    [Google Scholar]
  36. Rambourg A. 1971; Morphological and histochemical aspects of glycoprotein at the surface of animal cells. In International Review of Cytology, vol. 31, pp. 57–114 Edited by G. H. Bourne & J. F. Danielli. New York & London : Academic Press.
    [Google Scholar]
  37. Rosenberg M. 1981; Bacterial adherence to polystyrene: a replica method of screening for bacterial hydrophobicity. Applied and Environmental Microbiology 42:375–377
    [Google Scholar]
  38. Rosenberg M., Rottom S., Rosenberg G. 1982; Cell surface hydrophobicity of smooth and rough Proteus mirabilis strains as determined by adherence to hydrocarbons. FEMS Microbiology Letters 13:167–169
    [Google Scholar]
  39. Wardell J. N., Brown C. M., Ellwood D. C. 1980; A continuous culture study of the attachment of bacteria to surfaces. In Microbial Adhesion to Surfaces, pp. 221–230 Edited by R. C. W. Berkeley, J. M. Lynch, J. Melling, P. R. Rutter & B. Vincent. Chichester: Ellis Horwood.
    [Google Scholar]
  40. Zvyagintsev D. G. 1959; Adsorption of microorganisms by glass surfaces. Microbiology 20:104–108
    [Google Scholar]
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