1887

Abstract

Summary: A 5-bromo-2ʹ-deoxyuridine (BUdR)-tolerant derivative of a thymidine (TdR)-requiring strain of was used to examine the effect of BUdR, an analogue of TdR, on sporulation. At a TdR: BUdR ratio which had little effect on growth, sporulation was inhibited if cells were exposed to BUdR during the period of DNA synthesis at the onset of the process. Cells recovered from BUdR inhibition of sporulation if the analogue was removed and DNA replication allowed to continue with TdR alone. BUdR prolonged the period of DNA synthesis during sporulation and experiments with chloramphenicol suggested that this was due in part to unscheduled initiation of new rounds of replication.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-2-493
1986-02-01
2021-08-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/2/mic-132-2-493.html?itemId=/content/journal/micro/10.1099/00221287-132-2-493&mimeType=html&fmt=ahah

References

  1. Binnie C. 1982; The effect of 5-bromo-2ʹ-deoxyuridine on growth and sporulation in Bacillus suhtilis. PhD thesis, University of Glasgow, UK. Folia microbiologica 29:8–13
    [Google Scholar]
  2. Binnie C., Coote J. G. 1983; Density gradient analysis of DNA replicated during Bacillus subtilis sporulation. Journal of Bacteriology 156:466–470
    [Google Scholar]
  3. Biswas D. K., Abdullah K. T., Brennessel B. A. 1979; On the mechanism of 5-bromodeoxyuridine induction of prolactin synthesis in rat pituitary tumor cells. Journal of Cell Biology 81:1–19
    [Google Scholar]
  4. Brown N. C. 1970; 6-(p-Hydroxyphenylazo)-uracil: a selective inhibitor of host DNA replication in phage-infected Bacillus subtilis. Proceedings of the National Academy of Sciences of the United States of America 67:1451–1461
    [Google Scholar]
  5. Brown N. C. 1971; Inhibition of bacterial DNA replication by 6-(p-hydroxyphenylazo)-uracil: differential effect on repair and semi-conservative synthesis in Bacillus subtilis. Journal of Molecular Biology 59:1–16
    [Google Scholar]
  6. Conway W. F., Schwartz N. B., Dorfman A. 1982; The irreversible effect of bromodeoxyuridine on chick chondrocytes. Experimental Cell Research 139:79–86
    [Google Scholar]
  7. Coote J. G. 1977; Interference by bromodeoxyuridine with differentiation in a prokaryote. Nature, London 267:635–637
    [Google Scholar]
  8. Coote J. G., Binnie C. 1981; Association between DNA replication and interference by bromodeoxyuridine with sporulation in Bacillus subtilis. In Sporulation and Germination, pp. 157–162 Edited by H. S. Levinson, A. L. Sonenshein & D. J. Tipper. Washington, DC: American Society for Microbiology.
    [Google Scholar]
  9. Coote J. G., Binnie C. 1986; Tolerance to bromodeoxyuridine in a thymidine-requiring strain of Bacillus subtilis. Journal of General Microbiology 132:481–492
    [Google Scholar]
  10. Davidson R. L., Kaufmann E. R. 1977; Deoxy-cytidine reverses the suppression of pigmentation caused by 5-BrdUrd without changing the amount of 5-BrdUrd in DNA. Cell 12:923–929
    [Google Scholar]
  11. Dunn G., Jeffs P., Mann N. H., Torgersen D. M., Young M. 1978; The relationship between DNA replication and the induction of sporulation in Bacillus subtilis. Journal of General Microbiology 108:189–195
    [Google Scholar]
  12. Evans I., Distefano P., Case K. R., Bosmann H. B. 1977; Cell surface changes caused by growth of B16 melanoma cells in bromodeoxyuridine. FEBS Letters 78:109–112
    [Google Scholar]
  13. Garcia M., Westley B., Rochefort H. 1981; 5-Bromodeoxyuridine specifically inhibits the synthesis of estrogen-induced proteins in MCF7 cells. European Journal of Biochemistry 116:297–301
    [Google Scholar]
  14. Gordon J. S., Bell G. I., Martinson H. C., Rutter W. J. 1976; Selective interaction of 5ʹ-bromo-deoxyuridine substituted DNA with different chromosomal proteins. Biochemistry 15:4778–4786
    [Google Scholar]
  15. Hill B. T., Tsuboi A., Baserga R. 1974; Effect of 5-bromodeoxyuridine on chromatin transcription in confluent fibroblasts. Proceedings of the National Academy of Sciences of the United States of America 71:455–459
    [Google Scholar]
  16. Horn D., Davidson R. L. 1976; Inhibition of biological effects of bromodeoxyuridine by deoxycy-tidine: correlation with decreased incorporation of bromodeoxyuridine into DNA. Somatic Cell Genetics 2:469–481
    [Google Scholar]
  17. Hutchinson F., Hales H. B. 1970; Mechanism of the sensitisation of bacterial transforming DNA to ultraviolet light by the incorporation of 5-bromo-uracil. Journal of Molecular Biology 50:59–69
    [Google Scholar]
  18. Johnson W. C., Moran C. P., Losick R. 1983; Two RNA polymerase sigma factors from Bacillus subtilis discriminate between overlapping promoters for a developmentally regulated gene. Nature, London 302:800–804
    [Google Scholar]
  19. Koyama H., Ono T. 1972; Further studies on the induction of alkaline phosphatase by 5-bromodeoxyuridine in a hybrid line between mouse and Chinese hamster in culture. Biochimica et biophysica acta 264:497–507
    [Google Scholar]
  20. Lark K. G., Repko T., Hoffan E. J. 1963; The effect of amino acid deprivation on subsequent deoxyribonucleic acid replication. Biochimica et biophysica acta 76:9–24
    [Google Scholar]
  21. Levitt D., Dorfman A. 1972; The irreversible inhibition of differentiation of limb-bud mesenchyme by bromodeoxyuridine. Proceedings of the National Academy of Sciences of the United States of America 69:1253–1257
    [Google Scholar]
  22. Lin S., Lin D., Riggs A. D. 1976; Histones bind more tightly to bromodeoxyuridine-substituted DNA than to normal DNA. Nucleic Acids Research 3:2183–2191
    [Google Scholar]
  23. Losick R., Pero J. 1981; Cascades of sigma factors. Cell 25:582–584
    [Google Scholar]
  24. Lough J., Bischoff R. 1976; Differential sensitivity to 5-bromodeoxyuridine during the S-phase of synchronised myogenic cells. Developmental Biology 50:457–475
    [Google Scholar]
  25. Mandelstam J., Sterlini J. M., Kay D. 1971; Sporulation of Bacillus subtilis. Effect of medium on the form of chromosome replication and on initiation of sporulation in Bacillus subtilis. Biochemical Journal 125:635–641
    [Google Scholar]
  26. Mayne R., Abbot J., Holtzer H. 1973; Requirement for cell proliferation for the effects of 5-bromodeoxyuridine on cultures of chick chondrocytes. Experimental Cell Research 77:255–263
    [Google Scholar]
  27. Mendelson N. H., Gross J. D. 1967; Characterisation of a temperature-sensitive mutant of Bacillus subtilis defective in deoxyribonucleic replication. Journal of Bacteriology 94:1603–1608
    [Google Scholar]
  28. Miura Y., Wilt F. H. 1971; The effects of bromodeoxyuridine on yolk sac erythropoiesis in the chick embryo. Journal of Cell Biology 48:523–532
    [Google Scholar]
  29. O’Brien J. C. 1980; Tyrosine aminotransferase sensitivity to bromodeoxyuridine during restricted intervals of S-phase in Hepatoma cells. Journal of Cell Biology 87:629–632
    [Google Scholar]
  30. Oishi M., Yoshikawa H., Sueoka N. 1964; Synchronous and dichotomous replications of the Bacillus subtilis chromosome during spore germination. Nature, London 204:1069–1073
    [Google Scholar]
  31. Piggot P. J., Coote J. G. 1976; Genetic aspects of bacterial endospore formation. Bacteriological Reviews 40:908–962
    [Google Scholar]
  32. Reff M. E., Davidson R. C. 1979; Deoxycytidine reverses the suppression of pigmentation caused by 5-BrdUrd without changing the distribution of 5-BrdUrd in DNA. Journal of Biological Chemistry 254:6869–6872
    [Google Scholar]
  33. Rima B. K., Takahashi I. 1978; Synthesis of thymidine nucleotides in Bacillus subtilis. Canadian Journal of Biochemistry 56:158–160
    [Google Scholar]
  34. Rogers J., Ng S. K. C., Coulter M. B., Sanwal B. D. 1975; Inhibition of myogenesis in a rat myoblast line by 5-bromodeoxyuridine. Nature, London 256:438–440
    [Google Scholar]
  35. Rosenthal S. L., Parola A. H., Blout E. R., Davidson R. L. 1978; Membrane alterations associated with “transformation” by BUdR in BUdR-dependent cells. Experimental Cell Research 112:419–429
    [Google Scholar]
  36. Rutter W. J., Pictet R. C., Morris P. W. 1973; Toward molecular mechanisms of development processes. Annual Review of Biochemistry 42:601–646
    [Google Scholar]
  37. Sargent M. G. 1980; Chromosome replication in sporulating cells of Bacillus subtilis. Journal of Bacteriology 142:491–498
    [Google Scholar]
  38. Schubert D., Jacob F. 1970; 5-Bromodeoxyuri-dine induced differentiation of a neuroblastoma. Proceedings of the National Academy of Sciences of the United States of America 67:246–254
    [Google Scholar]
  39. Sterlini J. M., Mandelstam J. 1969; Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochemical Journal 113:29–37
    [Google Scholar]
  40. Stockdale F., Okazaki K., Nameroff M., Holtzer H. 1964; 5-Bromodeoxyuridine: effect on myogenesis in vitro. Science 146:533–535
    [Google Scholar]
  41. Sueoka N., Yoshikawa H. 1965; The chromosome of Bacillus subtilis. I. Theory of marker frequency analysis. Genetics 52:747–757
    [Google Scholar]
  42. Weintraub H., Campbell G. L., Holtzer H. 1972; Identification of a developmental program using bromodeoxyuridine. Journal of Molecular Biology 70:337–350
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-2-493
Loading
/content/journal/micro/10.1099/00221287-132-2-493
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error