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Volume 132, Issue 2

Tolerance to Bromodeoxyuridine in a Thymidine-requiring Strain of Free

Abstract

Summary: Mutations which allow tolerance to 5-bromo-2′-deoxyuridine (BUdR) in a thymidine (TdR)-requiring strain of have been examined. Differences in sensitivity to BUdR existed between isogenic strains harbouring the mutations. Those mutations originally isolated as BUdR-tolerant also bestowed tolerance to 5-bromouracil and vice versa. The strain exhibiting the greatest tolerance to BUdR maintained a normal rate of replication in the presence of BUdR whereas the parent strain did not, but the tolerant strain incorporated less analogue into DNA than the parent strain. The basis of the tolerance mutation appeared to lie at the point of uptake of the analogue into the cell as the tolerant mutant preferentially took up TdR over BUdR into whole cells. DNA polymerase activity measured did not distinguish between TdR and BUdR in either the parent or the mutant strain and although TdR kinase activity showed a preference for TdR over BUdR as a substrate, the extent of discrimination was similar in both strains.

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© Society for General Microbiology 1986
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1986-02-01
2024-04-20
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References

  1. Ayad S. R., Barker G. R. 1969; The integration of donor and recipient deoxyribonucleic acid during transformation of B. subtilis. Biochemical Journal 113:167–174
     [Google Scholar]
  2. Binnie C. 1982; PhD thesis. University of Glasgow, UK. The effect of 5-bromo-2-deoxyuridine on growth and sporulation in Bacillus subtilis.
     [Google Scholar]
  3. Binnie C., Coote J. G. 1983; Density gradient analysis of DNA replicated during Bacillus subtilis sporulation. Journal of Bacteriology 156:466–470
     [Google Scholar]
  4. Bishop R. J., Sueoka N. 1972; 5-Bromouracil-tolerant mutants of Bacillus subtilis. Journal of Bacteriology 112:870–876
     [Google Scholar]
  5. Bott K. F., Wilson G. A. 1967; Development of competence in the Bacillus subtilis transformation system. Journal of Bacteriology 94:562–570
     [Google Scholar]
  6. Bradley W. F. C.., Dinelle C., Charron J., Langelier Y. 1982; Bromodeoxyuridine resistance in CHO cells occurs in three discrete steps. Somatic Cell Genetics 8:207–222
     [Google Scholar]
  7. Callister H., Wake R. G. 1974; Completed chromosomes in thymine-requiring Bacillus subtilis spores. Journal of Bacteriology 120:579–582
     [Google Scholar]
  8. Cohen S. S., Barner H. D. 1956; Studies on the induction of thymine deficiency and on the effect of thymine and thymidine analogues in Escherichia coli. Journal of Bacteriology 71:588–597
     [Google Scholar]
  9. Coote J. G. 1972; Sporulation in Bacillus subtilis. Characterization of oligosporogenous mutants and comparison of their phenotypes with those of asporogenous mutants. Journal of General Microbiology 71:1–15
     [Google Scholar]
  10. Coote J. G. 1977; Interference by bromodeoxyuridine with differentiation in a prokaryote. Nature, London 267:635–637
     [Google Scholar]
  11. Coote J. G., Binnie C. 1981; Association between DNA replication and interference by bromodeoxyuridine with sporulation in Bacillus subtilis. Edited by H. S. Levinson, A. L. Sonenshein and D. J. Tipper. Washington, DC: American Society for Microbiology. In Sporulation and Germination pp:157–162
     [Google Scholar]
  12. Ephrati-Elizur E. 1968; Spontaneous transformation in Bacillus subtilis. Genetical Research 11:83–96
     [Google Scholar]
  13. Ephrati-Elizur E., Borenstein S. 1971; Velocity of chromosome replication in thymine-requiring and independent strains of Bacillus subtilis. Journal of Bacteriology 106:58–64
     [Google Scholar]
  14. Haas M., Yoshikawa H. 1969; Defective bacteriophage PBSH in Bacillus subtilis. I. Induction, purification and physical properties of the bacteriophage and its deoxyribonucleic acid. Journal of Virology 3:233–247
     [Google Scholar]
  15. Huang P. C., Eberle H., Boice L. B., Romig. W. R. 1968; Replication of Bacillus subtilis DNA during germination in 5-bromouracil containing medium and marker mapping. Genetics 60:661–672
     [Google Scholar]
  16. Laird C. D., Bodmer W. F. 1967; 5-Bromouracil utilisation by Bacillus subtilis. Journal of Bacteriology 94:1277–1278
     [Google Scholar]
  17. Lunn A., Cooke D., Haugli F. 1977; Genetics and biochemistry of 5-bromodeoxyuridine resistance in Physarum polycephalum. Genetical Research 30:1–12
     [Google Scholar]
  18. Meuth M., Green H. 1974; Induction of a deoxycytidineless state in cultured mammalian cells by bromodeoxyuridine. Cell 2:109–112
     [Google Scholar]
  19. Nagley P., Wake R. G. 1969; Effect of 5-bromouracil on the pattern of deoxyribonucleic acid replication in germinating Bacillus subtilis spores. Journal of Molecular Biology 43:619–630
     [Google Scholar]
  20. O'Donovan G. A., Neuhard J. 1970; Pyrimidine metabolism in microorganisms. Bacteriological Reviews 34:278–343
     [Google Scholar]
  21. Oishi M., Yoshikawa H., Sueoka N. 1964; Synchronous and dichotomous replication of the Bacillus subtilis chromosome during spore germination. Nature, London 204:1069–1073
     [Google Scholar]
  22. Okazaki R., Kornberg A. 1964; Deoxythymidine kinase of Escherichia coli. II. Kinetics and feedback control. Journal of Biological Chemistry 239:275–284
     [Google Scholar]
  23. Ostertag W., Crozier T., Kiuge. N., Melderis. H., Dube S. 1973; Action of 5-bromodeoxyuridine on the induction of haemoglobin synthesis in mouse leukaemia cells resistant to 5-BUdR. Nature New Biology 243:203–205
     [Google Scholar]
  24. O'Sullivan A., Howard K., Sueoka N. 1975; Location of a unique replication terminus and genetic evidence for partial bidirectional replication in the Bacillus subtilis chromosome. Journal of Molecular Biology 91:15–38
     [Google Scholar]
  25. Quinn W. G., Sueoka N. 1970; Symmetrical replication of the Bacillus subtilis chromosome. Proceedings of the National Academy of Sciences of the United States of America 67:717–723
     [Google Scholar]
  26. Rima B. K., Takahashi I. 1978; Synthesis of thymidine nucleotides in Bacillus subtilis. Canadian Journal of Biochemistry 56:158–160
     [Google Scholar]
  27. Rinehart K. V., Copeland J. C. 1973; Evidence that thymine is not a normal metabolite in wild-type Bacillus subtilis. Biochimica et biophysica acta 294:1–7
     [Google Scholar]
  28. Rogers J., Ng S. K. C., Coulter M. B., Sanwal. B. D. 1975; Inhibition of myogenesis in a rat myoblast line by 5-bromodeoxyuridine. Nature, London 256:438–440
     [Google Scholar]
  29. Sargent M. G. 1980 a; A procedure for isolating high quality DNA from spores of Bacillus subtilis 168. Journal of General Microbiology 116:511–514
     [Google Scholar]
  30. Sargent M. G. 1980 b; Chromosome replication in sporulating cells of Bacillus subtilis. Journal of Bacteriology 142:491–498
     [Google Scholar]
  31. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. Journal of Molecular Biology 4:430–443
     [Google Scholar]
  32. Seaman E., Tarmy E., Marmur J. 1964; Inducible phages of Bacillus subtilis. Biochemistry 3:607–613
     [Google Scholar]
  33. Sterlini J. M., Mandelstam J. 1969; Commitment to sporulation in Bacillus subtilis and its relationship to the development of actinomycin resistance. Biochemical Journal 113:29–37
     [Google Scholar]
  34. Sueoka N., Yoshikawa H. 1965; The chromosome of Bacillus subtilis. I. Theory of marker frequency analysis. Genetics 52:747–757
     [Google Scholar]
  35. Wake R. G., Baldwin R. L. 1962; Physical studies on the replication of DNA in vitro. Journal of Molecular Biology 5:201–216
     [Google Scholar]
  36. Ward J. B., Curtis C. A. M., Taylor C., Buxton R. S. 1982; Purification and characterisation of two phage PBSX-induced lytic enzymes of Bacillus subtilis 168. Journal of General Microbiology 128:1171–1178
     [Google Scholar]
  37. Warner F. D., Kitos G. A., Romano M. P., Hemphill H. E. 1977; Characterisation of SPβ: a temperate bacteriophage from Bacillus subtilis 168M. Canadian Journal of Microbiology 23:45–51
     [Google Scholar]
  38. Winston S., Matsushita T. 1975; Permanent loss of chromosome initiation in toluene-treated Bacillus subtilis cells. Journal of Bacteriology 123:921–927
     [Google Scholar]
  39. Yasbin R. E., Fields P. I., Anderson B. J. 1980; Properties of Bacillus subtilis 168 derivatives freed of their natural phages. Gene 12:155–159
     [Google Scholar]
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Tolerance to Bromodeoxyuridine in a Thymidine-requiring Strain of Bacillus subtilis
Microbiology 132, 481 (1986); https://doi.org/10.1099/00221287-132-2-481
/content/journal/micro/10.1099/00221287-132-2-481
/content/journal/micro/10.1099/00221287-132-2-481
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