1887

Abstract

Summary: Phenylalanineaminopeptidase was isolated and purified from the culture filtrate of by affinity chromatography on -tert-butyl-l-threonyl-l-phenylalanyl-l-prolyl-glycyl-aminosilochrom and by gel-filtration; a 401-fold purification with a yield of 18% was achieved. The enzyme was a metalloenzyme with a molecular weight of 35000 and a pI of 5.8. It was stable at pH 7–9 and had an activity optimum in the range of pH 8–9.5 with l-phenylalanine -nitroanilide as substrate. Enzyme activity was highest towards the latter compound, substantially lower towards l-leucine -nitroanilide and only marginal towards other -nitroanilides. Besides phenylalanineaminopeptidase, a metalloproteinase and a serine proteinase were also detected in culture filtrate.

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1986-02-01
2024-10-03
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References

  1. Andrews P. 1965; The gel filtration behaviour of proteins related to their molecular weights over a wide range. Biochemical Journal 96:595–606
    [Google Scholar]
  2. Berdal B. P., Fossum K. 1982; Occurrence and immunogenicity of proteinases from Legionella species. European Journal of Clinical Microbiology 1:7–11
    [Google Scholar]
  3. Berdal B. P., Hushovd O., Olsvik O., Odegard O. R., Bergan T. 1982a; Demonstration of extracellular proteolytic enzymes from Legionella species strains by using synthetic chromogenic peptide substrates. Acta pathologica et microbiologica scandinavica B90:119–123
    [Google Scholar]
  4. Berdal B. P., Olsvik O., Myhre S., Omland T. 1982b; Demonstration of extracellular chymotryp-sin-like activity from various Legionella species. Journal of Clinical Microbiology 16:452–457
    [Google Scholar]
  5. Berdal B. P., Bövre K., Olsvik O., Omland T. 1983; Patterns of extracellular proline-specific endopeptidases in Legionella and Flavobacterium species demonstrated by use of chromogenic peptides. Journal of Clinical Microbiology 17:970–974
    [Google Scholar]
  6. Davis B. J. 1964; Disc electrophoresis. II. Methods and application to human serum proteins. Annals of the New York Academy of Sciences 121:404–427
    [Google Scholar]
  7. Dod B. J., Balassa G., Raulet E., Jeannoda V. 1978; Spore control (Sco) mutations in Bacillus subtilis. II. Sporulation and the production of extracellular proteases and ot-amylase by Sco mutants. Molecular and General Genetics 163:45–56
    [Google Scholar]
  8. Fraser D. W., Tsai T. F., Orenstein W., the Field Investigation Team. . 1977; Legionnaires disease: description of an epidemic of pneumonia. New England Journal of Medicine 297:1189–1196
    [Google Scholar]
  9. George J. R., Pine L., Reeves M. W., Harrell W. K. 1980; Amino acid requirements of Legionella pneumophila. Journal of Clinical Microbiology 11:286–291
    [Google Scholar]
  10. Ivanova N. M., Vaganova T. 1., Strongin A. Ya., Stepanov V. M. 1977; Isolation and properties of leucineaminopeptidase from Aspergillus oryzae. Biokhimiya 42:843–849
    [Google Scholar]
  11. Kaluger S. V., Borovikova V. P., Lavrenova G. I., Stepanov V. M., Shpokene A. P., Gureeva M. P., Uzhkurenas A. P. 1983; Extracellular serine proteinase A of Streptomyces rutgersensis. Biokhimiya 48:1483–1490
    [Google Scholar]
  12. Kolehmainen L., Mikola J. 1971; Partial purification and enzymatic properties of an amino-peptidase from barley. Archives of Biochemistry and Biophysics 145:633–642
    [Google Scholar]
  13. Lyublinskaya L. A., Yakusheva L. D., Stepanov V. M. 1977; Synthesis of subtilisin peptide substrates and their analogs. Bioorganicheskaya khimiya 3:273–279
    [Google Scholar]
  14. Lyublinskaya L. A., Yakusheva M. P., Vaganova T. I., Ivanova N. M., Stepanov V. M. 1984; Solid-phase synthesis of biospecific sorbent for aminopeptidases. Bioorganicheskaya khimiya 10:1490–1495
    [Google Scholar]
  15. Merkef J. R., Lee C. C., Freund T. S. 1981; A dimeric extracellular heal stable aminopeptidase produced by a marine pseudomonad. Biochimica et biophysica acta 661:32–38
    [Google Scholar]
  16. Müller H. E. 1980; Proteolytic action of Legionella pneumophila. Infection and Immunity 34:299–302
    [Google Scholar]
  17. Müller H. E. 1981; Enzymatic profile of Legionella pneumophila. Journal of Clinical Microbiology 13:423–426
    [Google Scholar]
  18. Ninomiya K., Tanaka Sh., Kawata Sh., Maki-sumi S. 1981; Purification and properties of an aminopeptidase from seeds of Japanese apricot. Journal of Biochemistry 89:193–201
    [Google Scholar]
  19. Oksenoit E. S., Petrova E. N., Filippova I., Lavrenova G. I., Tarasova N. I., Stepanov V. M. 1983; Chromogenic α-chymotrypsin substrate pyroglutamyl-phenylalanine p-nitroanilide. Khimiya prirodnych soedineni 1:122–123
    [Google Scholar]
  20. Philippova I. Yu., Lysogorskaya E. N., Oksenoit E. S., Rudenskaya G. N., Stepanov V. M. 1984; l-Pyroglutamyl-l-phenylalanyl-l-leucine-p-nitroanilide - a chromogenic substrate for thiol proteinase assay. Analytical Biochemistry 142:293–297
    [Google Scholar]
  21. Prescott J. M., Wilkes S. H., Wagner F. W., Wilsan K. J. 1971; Aeromonas aminopeptidase. Improved isolation and some physical properties. Journal of Biological Chemistry 246:1756–1764
    [Google Scholar]
  22. Rodriguez-Absi J., Prescott J. M. 1978; Isolation and properties of an aminopeptidase from Bacillus licheniformis. Archives of Biochemistry and Biophysics 186:383–391
    [Google Scholar]
  23. Stepanov V. M., Chestukhina G. G., Rudenskaya G. N., Epremyan A. S., Osterman A. L., Khodova O. M., Belyanova L. P. 1981; A new subfamily of microbial serine proteinases? Structural similarities of Bacillus thuringiensis and Thermoactin-omyces vulgaris extracellular serine proteinases. Biochemical and Biophysical Research Communications 100:1680–1687
    [Google Scholar]
  24. Thompson M. R., Miller R. D., Iglewski B. H. 1981; In vitro production of an extracellular protease by Legionella pneumophila. Infection and Immunity 34:299–302
    [Google Scholar]
  25. Vaganova T. I., Ivanova N. M., Yusupova M. P., Stepanov V. M. 1983; Exopeptidases of mycelial fungi. In Mycelial Fungi (Physiology, Biochemistry. Biotechnology), Edited by E. L. Golov-lyev. Puschino: Nauchny Centr Biologicheskykh Issledovany. 116–117
    [Google Scholar]
  26. Vodkin L. O., Scandalious J. G. 1980; Comparative properties of genetically defined peptidases in maize. Biochemistry 19:4660–4667
    [Google Scholar]
  27. Wagner F. W., Wilkes S. H., Prescott J. M. 1972; Specificity of Aeromonas aminopeptidase toward amino acid amides and dipeptides. Journal of Biological Chemistry 247:1208–1210
    [Google Scholar]
  28. Waters S. P., Dalling M. J. 1984; Isolation and some properties of an aminopeptidase from the primary leaf of wheat(Triticum aestivum L. ). Plant Physiology 15:118–124
    [Google Scholar]
  29. Weber K., Osborn M. 1969; The reliability of molecular weight determination by dodecyl sulfate polyacrylamide gel electrophoresis. Journal of Biological Chemistry 224:4406–4412
    [Google Scholar]
  30. Wiersma M., Versteegh G., Assink H. A., Welling G. W., Harber W. 1978; Purification and some properties of two extracellular proteolytic enzymes, produced by Vibrio SAl. Antonie van Leeuwenhoek 44:157–169
    [Google Scholar]
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