1887

Abstract

Summary: Populations of NCYC 431, harvested after 16 h incubation from self-induced anaerobic cultures, were more tolerant to the inhibitory effect of ethanol on fermentation rate and viability than organisms harvested from 8 h cultures. Ethanol increased the rate of passive influx of protons into de-energized organisms at a rate which was greater with organisms from 8 h compared with 16 h cultures. Rates of passive influx of protons into spheroplasts were significantly greater than into intact organisms, although culture age did not affect rates of ethanol-induced influx of protons into spheroplasts. Ethanol retarded both the initial net rate of proton efflux and the final extent of acidification produced by suspensions of energized organisms, both effects being more pronounced with organisms from 8 h as compared with 16 h cultures. The magnitude of the proton-motive force (Δ) was decreased by ethanol in both energized and de-energized organisms. Although culture age did not affect the extent of ethanol-induced decrease in Δ in de-energized organisms, in energized organisms harvested from 8 h cultures ethanol produced a significantly greater decrease in Δ as compared with organisms from 16 h cultures. If the ability of ethanol to decrease the Δ value is important in its inhibitory effect on growth, it is suggested that some phenomenon other than proton uncoupling is involved.

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1986-02-01
2021-07-24
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References

  1. Alterthum F., Rose A. H. 1973; Osmotic lysis of spheroplasts from Saccharomyces cerevisiae grown anaerobically in media containing different unsaturated fatty acids. Journal of General Microbiology 77:371–382
    [Google Scholar]
  2. Barnett J. A., Sims A. P. 1976; Some physiological observations on the uptake of D-glucose and 2-deoxy-D-glucose by starving and exponentially growing yeasts. Archives of Microbiology 111:185–192
    [Google Scholar]
  3. Beavan M. J., Charpentier C., Rose A. H. 1982; Production and tolerance of ethanol in relation to phospholipid fatty-acyl composition in Saccharomyces cerevisiae NCYC 431. Journal of General Microbiology 128:1447–1455
    [Google Scholar]
  4. Borst-Pauwels G. W. F. H. 1981; Ion transport in yeast. Biochimica et biophysica acta 650:88–127
    [Google Scholar]
  5. Calderbank J., Keenan M. H. J., Rose A. H., Holman G. D. 1984; Accumulation of amino acids by Saccharomyces cerevisiae Y185 with phospholipids enriched in different fatty-acyl residues: a statistical analysis of data. Journal of General Microbiology 130:2817–2824
    [Google Scholar]
  6. Conway E. J., Downey M. 1950; pH values of the yeast cell. Biochemical Journal 47:355–360
    [Google Scholar]
  7. Davies C. W. 1962; Ion Association, London & Boston: Butterworth. 39–43
    [Google Scholar]
  8. Eddy A. A. 1982; Mechanisms of solute transport into selected eukaryotic micro-organisms. Advances in Microbial Physiology 23:1–78
    [Google Scholar]
  9. Eilam Y. 1984; Effects of phenothiazines on inhibition of plasma membrane ATPase and hyperpolarization of cell membranes in the yeast Saccharomyces cerevisiae. . Biochimica et biophysica acta 769:601–610
    [Google Scholar]
  10. Fink H., Kühles R. 1933; Beiträge zur Methyl-lenblaufärburg der Hefezellen und Studien über die Permeabilität der Hefezellmembran. II. Mitteilung. Eine Verbasserte Färbflüssigkeit zur Erkennung von totan Hefezellen. Hoppe-Seyler’s Zeitschrift fur physiologische Chemie 218:65–66
    [Google Scholar]
  11. Hauer R., Höfer M. 1978; Evidence for interactions between the energy-dependent transport of sugars and the membrane potential in the yeast Rhodotorula gracilis (Rhodosporidium toruloides). Journal of Membrane Biology 43:335–349
    [Google Scholar]
  12. Hauer R., Uhlemann G., Neumann J., Höfer M. 1981; Proton pumps of the plasmalemma of the yeast Rhodotorula gracilis. Their coupling to fluxes of potassium and other ions. Biochimica et biophysica acta 649:680–690
    [Google Scholar]
  13. Höfer M., Künemund A. 1984; Tetraphenylphos-phonium ion is a true indicator of negative plasmamembrane potential in the yeast Rhodotorula glutinis. . Biochemical Journal 255:815–819
    [Google Scholar]
  14. Ingram L. O., Buttke T. M. 1984; Effects of alcohols on micro-organisms. Advances in Microbial Physiology 25:253–300
    [Google Scholar]
  15. Leão C., van Uden N. 1982; Effect of ethanol and other alkanols on the glucose transport system of Saccharomyces cerevisiae. Biotechnology and Bioengineering 24:2601–2604
    [Google Scholar]
  16. Leão C., van Uden N. 1983; Effect of ethanol and other alkanols on the ammonium transport system of Saccharomyces cerevisiae. Biotechnology and Bioengineering 25:2085–2090
    [Google Scholar]
  17. Leão C., van Uden N. 1984a; Effect of ethanol and other alkanols on the general amino acid permease of Saccharomyces cerevisiae. Biotechnology and Bioengineering 26:403–405
    [Google Scholar]
  18. Leão C., van Uden N. 1984b; Effects of ethanol and other alkanols on passive proton influx in the yeast Saccharomyces cerevisiae. Biochimica et biophysica acta 774:43–48
    [Google Scholar]
  19. Lobo Z., Maitra P. K. 1977; Resistance to 2-deoxyglucose in yeast. A direct selection of mutants lacking glucose-phosphorylating enzymes. Molecular and General Genetics 157:297–300
    [Google Scholar]
  20. Loureiro-Dias M. C., Peinado J. M. 1982; Effect of ethanol and other alkanols on the maltose transport system of Saccharomyces cerevisiae. Biotechnology Letters 4:721–724
    [Google Scholar]
  21. Malpartida F., Serrano R. 1981; Proton translocation catalysed by the purified yeast plasma membrane ATPase reconstituted in liposomes. FEBS Letters 131:351–354
    [Google Scholar]
  22. Rose A. H. 1980; Recent research on industrially important strains of Saccharomyces cerevisiae. In Biology and Activity of Yeasts, Edited by F. A. Skinner, S. M. Passmore & R. R. Davenport. London: Academic Press. 103–121
    [Google Scholar]
  23. Sanders D., Hansen U.-P., Slayman C. L. 1981; Role of the plasma membrane proton pump in pH regulation in non-animal cells. Proceedings of the National Academy of Sciences of the United States of America 78:5093–5907
    [Google Scholar]
  24. Seaston A., Carr C., Eddy A. A. 1976; The concentration of glycine by preparations of the yeast Saccharomyces cerevisiae depleted of adenosine triphosphate. Biochemical Journal 154:669–676
    [Google Scholar]
  25. Serrano R. 1977; Energy requirements for maltose transport in yeast. European Journal of Biochemistry 80:97–102
    [Google Scholar]
  26. Sigler K., Knotkovâ A., Kotyk A. 1981; Factors governing substrate-induced generation and extrusion of protons in the yeast Saccharomyces cerevisiae. . Biochimica et biophysica acta 643:572–582
    [Google Scholar]
  27. Thomas D. S., Rose A. H. 1979; Inhibitory effect of ethanol on growth and solute accumulation by Saccharomyces cerevisiae as affected by plasmamembrane lipid composition. Archives of Microbiology 122:49–55
    [Google Scholar]
  28. Thomas D. S., Hossack J. A., Rose A. H. 1978; Plasma-membrane lipid composition and ethanol tolerance in Saccharomyces cerevisiae. . Archives of Microbiology 117:239–245
    [Google Scholar]
  29. Umbreit W. W., Burris R. H., Stauffer J. F. 1964; Manometric Techniques, 4th ed. Minneapolis: Burgess Publishing Company.
    [Google Scholar]
  30. Waddell W. J., Butler T. C. 1959; Calculation of intracellular pH from the distribution of 5,5-dimethyl-2,4-oxazolidinedione (DMO). Application to skeletal muscle of the dog. Journal of Clinical Investigtion 38:720–729
    [Google Scholar]
  31. Wickerham L. J. 1951; Taxonomy of yeasts. I. Techniques of classification. United States Department of Agriculture Technical Bulletin no. 1029, Washington, DC: US Department of Agriculture.
    [Google Scholar]
  32. Willsky G. R. 1979; Characteristics of the plasma membrane Mg2+-ATPase from the yeast Saccharomyces cerevisiae. Journal of Biological Chemistry 254:3326–3332
    [Google Scholar]
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