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1887

Microbiology Society logo Microbiology

Volume 132, Issue 2

Operon of : Cloning and Sequence Free

Abstract

Summary: The locus , involved in the sporulation of , was cloned into derivatives of the temperate phage 105. Two recombinant phages were obtained which contain chromosomal DNA covering 1∙6 kbp. They are both able to complement mutations and . These mutations, which were believed to be in different loci, and respectively, were shown to be closely linked, and both map at the position assigned to on the genetic map of The sequence of 1656 bp carrying the locus was determined. Only one open reading frame was found; this codes for a protein of 343 amino acids. It is preceded by a ribosome binding site and possible recognition sequences for σ- and σ-RNA polymerases. Studies of the locus by means of integrational plasmid vectors defined the outer limits of the transcriptional unit. These results are completely compatible with the sequence data. The combination of sequence and mapping and the information obtained by the use of integrational plasmids confirm that the locus functions as a monocistronic operon.

  • Received:
  • Revised:
  • Published Online:
© Society for General Microbiology 1986
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1986-02-01
2025-01-13
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/content/journal/micro/10.1099/00221287-132-2-341
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spoIID Operon of Bacillus subtilis: Cloning and Sequence
Microbiology 132, 341 (1986); https://doi.org/10.1099/00221287-132-2-341
/content/journal/micro/10.1099/00221287-132-2-341
/content/journal/micro/10.1099/00221287-132-2-341
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