%0 Journal Article %A Gibbons, Gwendolyn F. %A Howard, Dexter H. %T Arginine Auxotrophs of Candida albicans Deficient in Argininosuccinate Lyase %D 1986 %J Microbiology, %V 132 %N 2 %P 263-268 %@ 1465-2080 %R https://doi.org/10.1099/00221287-132-2-263 %I Microbiology Society, %X Summary: Auxotrophic mutants of Candida albicans FC18 were induced by a combination of treatments with nitrous acid and UV irradiation. Arginine (Arg−), histidine (His−) and methionine/cysteine (MetA−) auxotrophs were recovered by this means. The Arg− auxotrophs lacked active argininosuccinate lyase (EC 4.3.2.1), the enzyme catalysing the final step in arginine biosynthesis. Thus the locus may be designated arg-4. The mutant strains bearing this mutation did not form germ tubes unless the germination medium contained arginine. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-132-2-263