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Volume 132, Issue 2

The Use of Activities of Carbon Catabolic Enzymes as a Probe for the Carbon Nutrition of Snakebean Nodule Bacteroids Free

Abstract

Summary: In sugar-grown cells of cowpea strain NGR234 activities for enzymes of the Entner-Doudoroff and pentose phosphate pathways were present while the virtual absence of phospho-fructokinase and fructose-bisphosphate aldolase indicated that the Embden–Meyerhof–Parnas pathway was unlikely to be significant. Invertase, fructokinase, glucose-6-phosphate dehydrogenase and the Entner–Doudoroff enzymes were present at only low activities in succinate grown cells, but were induced in sugar-grown cells. Isolated snakebean bacteroids contained very low activities of these four enzymes. Although C-dicarboxylic acids exerted some repressive effect on induction of these enzymes, there was substantial enzyme activity induced in cells grown on sucrose plus a C dicarboxylic acid. The data suggest that the peribacteroid membrane may be relatively impermeable to sugars and so dictate the carbon source(s) available to the bacteroids.

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© Society for General Microbiology 1986
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1986-02-01
2025-04-18
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/content/journal/micro/10.1099/00221287-132-2-243
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The Use of Activities of Carbon Catabolic Enzymes as a Probe for the Carbon Nutrition of Snakebean Nodule Bacteroids
Microbiology 132, 243 (1986); https://doi.org/10.1099/00221287-132-2-243
/content/journal/micro/10.1099/00221287-132-2-243
/content/journal/micro/10.1099/00221287-132-2-243
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