Stabilization of Glucose-starved K12 and LT2 by Peptidase-deficient Mutants Free

Abstract

Summary: K12 and LT2 cells were stabilized during carbon starvation in the presence of peptidase-deficient mutant strains. The rate of loss of viability of the wild-type strain was decreased an average of 2-fold, and the rate for the wild-type strain was decreased about 2.3-fold, when either was starved in the presence of the multiply peptidase-deficient strain TN852; other peptidase-deficient strains exhibited similar stabilizing effects. Starving wild-type LT2 cells utilized peptides excreted by the starving peptidase-deficient cells for protein synthesis, and, to a lesser extent, as respiratory substrates. Provision of free amino acids in steady-state levels to starving K12 cells in a cell recycle apparatus had a stabilizing effect similar to that of mixing with peptidase-deficient cells.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-2-231
1986-02-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/2/mic-132-2-231.html?itemId=/content/journal/micro/10.1099/00221287-132-2-231&mimeType=html&fmt=ahah

References

  1. Groat R. G., Matin A. 1986; Synthesis of unique proteins at the onset of carbon starvation in Escherichia coli. Journal of Industrial Microbiology 1:
    [Google Scholar]
  2. Inloes D. S. 1982; Immobilization of bacteria! and yeast cells in hollow fiber membrane bioreactors. PhD thesis, Stanford University.
    [Google Scholar]
  3. Inloes D. S., Smith W. J., Taylor D. P., Cohen S. N., Michaels A. S., Robertson C. R. 1983; Hollow-fiber membrane bioreactors using immobilized E. coli for protein synthesis. Biotechnology and Bioengineering 25:2653–2682
    [Google Scholar]
  4. Matin A., Veldhuis C., Stegeman V., Veenhuis M. 1979; Selective advantage of a Spirillum sp. in a carbon-limited environment. Accumulation of poly-β-hydroxybutyric acid and its role in starvation. Journal of General Microbiology 112:349–355
    [Google Scholar]
  5. Payne J. W. 1980; Transport and utilization of peptides by bacteria. In Microorganisms and Nitrogen Sources: Transport and Utilization of Amino Acids, Peptides, Proteins, and Related Substrates, pp. Edited by J. W. Payne. New York: John Wiley & Sons. 211–256
    [Google Scholar]
  6. Reeve C. A., Bockman A. T., Matin A. 1984a; Role of protein degradation in the survival of carbon-starved Escherichia coli and Salmonella typhimurium. Journal of Bacteriology 157:758–763
    [Google Scholar]
  7. Reeve C. A., Amy P. S., Matin A. 1984b; Role of protein synthesis in the survival of carbon-starved Escherichia coli K.-12. Journal of Bacteriology 160:1041–1046
    [Google Scholar]
  8. Yen C., Green L., Miller C. G. 1980; Degradation of intracellular protein in Salmonella typhimurium peptidase mutants. Journal of Molecular Biology 143:21–33
    [Google Scholar]
  9. Zychlinsky E., Matin A. 1983; Effect of starvation on cytoplasmic pH, proton motive force, and viability of an acidophilic bacterium, Thiobacillus acidophilus. Journal of Bacteriology 153:371–374
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-2-231
Loading
/content/journal/micro/10.1099/00221287-132-2-231
Loading

Data & Media loading...

Most cited Most Cited RSS feed