Summary: Two comptible mating-tyes of RSD4 and MA185, have recently been shown to have different surface properties by using aqueous two-phase partition. Changes in the surface properties of RSD4 amoebae were detected when in contact with cell culture medium from MA185 amoebae. The ability of MA185 culture medium to alter the surface of RSD4 amoebae was not affected by autoclaving or passage through a filter that removed molecules of greater than 10000. Activity was detected in medium from MA185 amoebae that had not previously been in contact with RSD4 amoebae. These results provide evidence for the existence of a low- factor produced by MA185 amoebae which affects the surface of RSD4 amoebae.


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