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Summary: Desulfovibrio vulgaris (Hildenborough) was grown on lactate with either a N2/CO2 or a H2/CO2 gas phase. H2 increased the growth yield on lactate and had a sparing effect on lactate utilization, without altering the growth rate or hydrogenase level. Growth on acetate plus CO2 with H2 as sole energy source did not require an extensive adaptation period. Addition of lactate to cultures growing on acetate and H2/CO2 resulted in a switch from acetate to lactate utilization. In lactate-limited medium under H2/CO2 biphasic growth was observed. Lactate was oxidized first with production of acetate, followed by a second phase of growth on the acetate. Under this condition H2 did not provide any supplementary energy during growth on lactate, as was evident from the ratio of lactate utilized to acetate produced.
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