Summary: (Hildenborough) was grown on lactate with either a N/CO or a H/CO gas phase. H increased the growth yield on lactate and had a sparing effect on lactate utilization, without altering the growth rate or hydrogenase level. Growth on acetate plus CO with H as sole energy source did not require an extensive adaptation period. Addition of lactate to cultures growing on acetate and H/CO resulted in a switch from acetate to lactate utilization. In lactate-limited medium under H/CO biphasic growth was observed. Lactate was oxidized first with production of acetate, followed by a second phase of growth on the acetate. Under this condition H did not provide any supplementary energy during growth on lactate, as was evident from the ratio of lactate utilized to acetate produced.


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