1887

Abstract

Summary: The regulation of the NH -assimilating enzymes glutamate dehydrogenase (GDH), glutamine synthetase (GS) and glutamate synthase (GOGAT), and the cellular composition of ZV620 grown in a chemostat with methanol and NH as the supplied C- and N-sources were investigated. The influence of either C- or N-limitation (as a function of dilution rate) and of the C: N ratio (at a constant growth rate) was studied. NADP-dependent GDH was active at high NH -concentrations and was repressed at low NH -concentrations. The activity increased with increasing dilution rates under C-limited growth conditions. Derepression of NADP-dependent GDH was observed at low dilution rates under N-limited growth conditions. GS was more active at low NH -concentrations where both the total enzyme level (deadenylylated plus adenylylated forms) and the active fraction increased. C-limited growth resulted in low activities of GS, whereas activity in N-limited cells was consistently high. Dilution rate did not have a significant influence on the specific activity. The specific activity of GOGAT increased with decreasing NH concentrations. Under both C- and N-limitation the specific activity of GOGAT increased with increasing growth rates. The C-content of the cells changed very little under the various growth conditions tested. The N-content and the protein content of the cells did not alter under C-limitation. N-limited growth conditions caused the cells to accumulate poly β-hydroxybutyrate. As a consequence, both the N-content and the protein content of the cells decreased.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-12-3337
1986-12-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/12/mic-132-12-3337.html?itemId=/content/journal/micro/10.1099/00221287-132-12-3337&mimeType=html&fmt=ahah

References

  1. Bender R. A., Janssen K. A., Resnick A. D., Blumenberg M., Foor F., Magasanik B. 1977; Biochemical parameters of glutamine synthetase from Klebsiella aerogenes . Journal of Bacteriology 129:1001–1009
    [Google Scholar]
  2. Brenchley J. E., Prival M. J., Magasanik B. 1973; Regulation of the synthesis of enzymes responsible for glutamate formation in Klebsiella aerogenes . Journal of Biological Chemistry 248:6122–6128
    [Google Scholar]
  3. Brown C. M. 1976; Nitrogen metabolism in bacteria and fungi. In Continuous Culture 6: Applications and New Fields pp 170–183 Edited by Dean A. C. R., Ellwood D. C., Evans C. G. T., Melling J. Chichester: Ellis Horwood;
    [Google Scholar]
  4. Brown C. M. 1980; Ammonia assimilation and utilization in bacteria and fungi. In Microorganisms and Nitrogen Sources pp 511–535 Edited by Payne J. W. Chichester: J. Wiley;
    [Google Scholar]
  5. Bushnell L. D., Haas H. F. 1941; The utilization of certain hydrocarbons by microorganisms. Journal of Bacteriology 41:653–673
    [Google Scholar]
  6. Dalton H. 1979; Utilization of inorganic nitrogen by microbial cells. International Review of Biochemistry 21:227–266
    [Google Scholar]
  7. Egli T., Quayle J. R. 1986; Influence of the carbon: nitrogen ratio of the growth medium on the cellular composition and the ability of the methylotrophic yeast Hansenula polymorpha to utilize mixed carbon sources. Journal of General Microbiology 132:1779–1788
    [Google Scholar]
  8. Harder W., Attwood M. M. 1978; Biology, physiology and biochemistry of Hyphomicrobia. Advances in Microbial Physiology 17:303–359
    [Google Scholar]
  9. Herbert D. 1961; The chemical composition of micro-organisms as a function of their environment. Symposium of the Society for General Microbiology 11:391–416
    [Google Scholar]
  10. Herbert D., Phipps P. J., Strange R. E. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5B:209–344
    [Google Scholar]
  11. Hueting S., Tempest D. W. 1979; Influence of the glucose input concentration on the kinetics of metabolite production by Klebsiella aerogenes NCTC 418 growing in chemostat culture in potassium- or ammonia-limited environments. Archives of Microbiology 123:189–194
    [Google Scholar]
  12. Janssen K. A., Magasanik B. 1977; Glutamine synthetase of Klebsiella aerogenes: genetic and physiological properties of mutants in the adenylylation system. Journal of Bacteriology 129:993–1000
    [Google Scholar]
  13. Karr D. B., Waters J. K., Emerich D. W. 1983; Analysis of poly-β-hydroxybutyrate in Rhizobium japonicum bacteroids by ion-exclusion high-pressure liquid chromatography and UV detection. Applied and Environmental Microbiology 46:1339–1344
    [Google Scholar]
  14. Kavanagh B. M., Cole J. A. 1976; The regulation of nitrogen metabolism in Escherichia coli . In Continuous Culture 6: Applications and New Fields pp 184–194 Edited by Dean A. C. R., Ellwood D. C., Evans C. G. T., Melling J. Chichester: Ellis Horwood;
    [Google Scholar]
  15. Kumar S., Nicholas D. J. D. 1983; Purification, properties and regulation of glutamine synthetase from Nitrobacter agilis . Journal of General Microbiology 130:959–966
    [Google Scholar]
  16. Loginova N.V., Shishkina V. N., Trotsenko Yu. A. 1976; Primary metabolic pathways of methylated amines in Hyphomicrobium vulgare . Microbiology (English translation of Microbiologiya) 45:34–40
    [Google Scholar]
  17. Magasanik B. 1982; Genetic control of nitrogen assimilation in bacteria. Annual Review of Genetics 16:135–168
    [Google Scholar]
  18. Meiberg J. B. M., Bruinenberg P. M., Harder W. 1980; Effect of dissolved oxygen tension on the metabolism of methylated amines in Hyphomicrobium X in the absence and presence of nitrate: evidence for ‘aerobic’ denitrification. Journal of General Microbiology 120:453–463
    [Google Scholar]
  19. Murell J. C., Dalton H. 1983; Ammonia assimilation in Methylococcus capsulatus (Bath) and other obligate methanotrophs. Journal of General Microbiology 129:1197–1206
    [Google Scholar]
  20. Nurse G. R. 1980; Denitrification with methanol: microbiology and biochemistry. Water Research 14:531–537
    [Google Scholar]
  21. Senior P. J. 1975; Regulation of nitrogen metabolism in Escherichia coli and Klebsiella aerogenes: studies with the continuous-culture technique. Journal of Bacteriology 123:407–418
    [Google Scholar]
  22. Shapiro B. M., Stadtman E. R. 1970; Glutamine synthetase (Escherichia coli). Methods in Enzymology 17A:910–922
    [Google Scholar]
  23. Sperl G. T., Hoare D. S. 1971; Denitrification with methanol: a selective enrichment for Hyphomicrobium species . Journal of Bacteriology 108:733–736
    [Google Scholar]
  24. Topiwala H., Hamer G. 1971; Effect of wall growth in steady-state continuous cultures. Biotechnology and Bioengineering 13:919–922
    [Google Scholar]
  25. Uebayasi M., Tonomura K. 1976; Denitrification by Hyphomicrobium capable of utilizing methanol. Journal of Fermentation Technology 54:885–890
    [Google Scholar]
  26. Wilkinson T. G., Hamer G. 1972; Some growth characteristics of a Hyphomicrobium sp. in batch culture. Journal of Applied Bacteriology 35:577–588
    [Google Scholar]
  27. Wilkinson T. G., Topiwala H. H., Hamer G. 1973; Interactions in a mixed bacterial population growing on methane in continuous culture. Biotechnology and Bioengineering 16:41–59
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-12-3337
Loading
/content/journal/micro/10.1099/00221287-132-12-3337
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error