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Volume 132, Issue 12

Transcript Analysis of the Citrate Synthase and Succinate Dehydrogenase Genes of K12 Free

Abstract

Summary: A transcript analysis of the citrate synthase and succinate dehydrogenase genes () of was done by nuclease S1 mapping. Evidence was obtained for two monocistronic transcripts extending anti-clockwise, to a common terminus, from independent promoters with start points 196 bp (major) and 299 bp (minor) upstream of the coding region. Evidence was also obtained for two polycistronic transcripts, (minor) and (major), extending clockwise, from sites 219 bp upstream of and 1455 bp upstream of (i.e. within ), to a common terminus. The synthesis of all of the transcripts was repressed by growth in the presence of glucose, and this is consistent with the well-established fact that both enzymes are subject to catabolite repression. Sequences resembling known binding sites for the cAMP-CRP (cyclicAMP-cyclicAMP receptor protein) complex occur in the vicinity of each promoter suggesting that they are activated by the cAMP-CRP complex.

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© Society for General Microbiology 1986
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1986-12-01
2024-04-19
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Transcript Analysis of the Citrate Synthase and Succinate Dehydrogenase Genes of Escherichia coli K12
Microbiology 132, 3239 (1986); https://doi.org/10.1099/00221287-132-12-3239
/content/journal/micro/10.1099/00221287-132-12-3239
/content/journal/micro/10.1099/00221287-132-12-3239
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