1887

Abstract

SUMMARY: Acid phosphatase, esterases, and glutamate, lactate and malate dehydrogenases of 34 strains of and 22 strains of were analysed by horizontal polyacrylamide agarose gel electrophoresis and by isoelectrofocusing in thin-layer polyacryl-amide gel. The two species could be separated on the basis of distinct electrophoretic patterns of all enzymes analysed. Glutamate dehydrogenase and acid phosphatase were detected exclusively in , whereas esterase bands were more intensively stained in . For each species, two zymotypes could be distinguished, on the basis of electrophoretic mobilities of malate dehydrogenase and banding patterns of esterase for , and by both isoelectric point and electrophoretic mobilities of an esterase and of lactate and malate dehydrogenases for . The high degree of enzyme polymorphism within the two species permitted precise identification of strains. The variations in electrophoretic patterns might therefore provide useful epidemiological markers.

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1986-11-01
2022-01-24
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