RT Journal Article SR Electronic(1) A1 Turner, Sheila M. A1 Mandelstam, JoelYR 1986 T1 Cloning and Sequencing of a Gene from Bacillus amyloliquefaciens that Complements Mutations of the Sporulation Gene spoIID in Bacillus subtilis JF Microbiology, VO 132 IS 11 SP 3025 OP 3035 DO https://doi.org/10.1099/00221287-132-11-3025 PB Microbiology Society, SN 1465-2080, AB SUMMARY: A segment of DNA from Bacillus amyloliquefaciens, which complemented a mutant sporulation gene, spoIID68, in Bacillus subtilis, was cloned into a derivative of the temperate bacteriophage ϕ105. The segment of DNA included an entire structural gene and complemented the mutation spoIID298, in addition to spoIID68, in B. subtilis. The nucleotide sequence of the gene from B. amyloliquefaciens was determined and compared with that of the B. subtilis gene; 74% homology was found in the coding region. Amino acid primary sequences derived from the nucleotide sequences of the two genes were also compared. The gene from B. amyloliquefaciens coded for a protein of 344 amino acid residues, one more than the protein coded by the corresponding gene from B. subtilis. Comparison of the primary amino acid sequences of the two genes showed that 78% of the residues were completely conserved and 8% were semi-conserved. Variation, however, was not random, i.e. some segments were much more highly conserved than others. Both proteins had a hydrophobic region at the N-terminus., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-132-11-3025