The Nucleotide Sequence and the Transcription During Sporulation of the Gene of Free

Abstract

SUMMARY: We have determined the nucleotide sequence of a 1496 bp stretch of chromosome that complements the mutation. The sequence contains three open reading frames. One of these is part of the gene, the other two, whose functions are unknown, are capable of encoding proteins with values of approximately 17000 and 8500. The use of integrational plasmids to delimit the transcriptional unit shows that the locus consists of one gene, which encodes a polypeptide of 74 amino acid residues and which is switched on after during sporulation of wild-type 168.

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1986-11-01
2024-03-28
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References

  1. Anagnostopoulos C., Spizizen J. 1961; Requirements for transformation in Bacillus subtilis. Journal of Bacteriology 81:741–746
    [Google Scholar]
  2. Biggin M. D., Gibson T. J., Hong G. F. 1983; Buffer gradient gel and 35S-label as an aid to rapid DNA sequence determination. Proceedings of the National Academy of Sciences of the United States of America 80:3963–3965
    [Google Scholar]
  3. Brennan S. M., Geiduschek E. P. 1983; Regions specifying transcriptional termination and pausing in the bacteriophage SPO1 terminal repeat. Nucleic Acids Research 11:4157–4175
    [Google Scholar]
  4. Casadaban M. J., Cohen S. N. 1980; Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. Journal of Molecular Biology 138:179–207
    [Google Scholar]
  5. Errington J. 1984; Efficient Bacillus subtilis cloning system using bacteriophage vector ?105J9. Journal of General Microbiology 130:2615–2628
    [Google Scholar]
  6. Fort P., Errington J. 1985; Nucleotide sequence and complementation analysis of a polycistronic sporulation operon, spoVA, in Bacillus subtilis. Journal of General Microbiology 131:1091–1105
    [Google Scholar]
  7. Fort P., Piggot P. J. 1984; Nucleotide sequence of sporulation locus spoIIA in Bacillus subtilis. Journal of General Microbiology 130:2147–2153
    [Google Scholar]
  8. Gilman M. Z., Wiggs J. L., Chamberlin M. J. 1981; Nucleotide sequences of two Bacillus subtilis promoters used by Bacillus subtilis sigma-28 RNA polymerase. Nucleic Acids Research 9:5991–6000
    [Google Scholar]
  9. Grunstein M., Hogness D. 1975; Colony hybridisation: a method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of Sciences of the United States of America 72:3961–3965
    [Google Scholar]
  10. Hasnain S., Sammons R., Roberts I., Thomas C. 1985; Cloning and deletion analysis of a genomic segment of Bacillus subtilis coding for the sdhA, B, C (succinate dehydrogenase) and gerE (spore germination) loci. Journal of General Microbiology 131:2269–2279
    [Google Scholar]
  11. Hederstedt L., Magnusson K., Rutberg L. 1982; Reconstitution of succinate dehydrogenase in Bacillus subtilis by protoplast fusion. Journal of Bacteriology 152:157–165
    [Google Scholar]
  12. Irie R., Okamoto T., Fujita Y. 1982; A germination mutant of Bacillus subtilis deficient in response to glucose. Journal of General and Applied Microbiology 28:345–354
    [Google Scholar]
  13. James W., Mandelstam J. 1985; Protease production during sporulation of germination mutants of Bacillus subtilis and the cloning of a functional gerE gene. Journal of General Microbiology 131:2421–2430
    [Google Scholar]
  14. Jenkinson H. F. 1981; Germination and resistance defects in spores of a Bacillus subtilis mutant lacking a coat polypeptide. Journal of General Microbiology 127:81–91
    [Google Scholar]
  15. Jenkinson H. F. 1983; Altered arrangement of proteins in the spore coat of a germination mutant of Bacillus subtilis. Journal of General Microbiology 129:1945–1958
    [Google Scholar]
  16. Jenkinson H. F., Lord H. 1983; Protease deficiency and its association with defects in spore coat structure, germination and resistance properties in a mutant of Bacillus subtilis. Journal of General Microbiology 129:2727–2737
    [Google Scholar]
  17. Jenkinson H. F., Mandelstam J. 1983; Cloning of the Bacillus subtilis lys and spoIIIB genes in phage 105. Journal of General Microbiology 129:2229–2240
    [Google Scholar]
  18. Jenkinson H. F., Sawyer W. D., Mandelstam J. 1981; Synthesis and order of assembly of spore coat proteins in Bacillus subtilis. Journal of General Microbiology 123:1–16
    [Google Scholar]
  19. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. McLaughlin J. R., Murray C. L., Rabinowitz J. C. 1981; Unique features in the ribosome binding site sequence of the Gram-positive Staphylococcus aureus β-lactamase gene. Journal of Biological Chemistry 256:11283–11291
    [Google Scholar]
  21. Messing J. 1983; New M13 vectors for cloning. Methods in Enzymology 101:20–78
    [Google Scholar]
  22. Messing J., Crea R., Seeburg P. H. 1981; A system for shotgun DNA sequencing. Nucleic Acids Research 9:309–321
    [Google Scholar]
  23. Moir A. 1981; Germination properties of a spore coat-defective mutant of Bacillus subtilis. Journal of Bacteriology 146:1106–1116
    [Google Scholar]
  24. Ohné M., Rutberg B., Hoch J. 1973; Genetic and biochemical characterisation of mutants of B. subtilis defective in succinate dehydrogenase. Journal of Bacteriology 115:738–745
    [Google Scholar]
  25. Rigby P. W. J., Diekmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  26. Rosenberg M., Court D. 1979; Regulatory sequences involved in the promotion and termination of RNA transcription. Annual Reviews of Genetics 13:319–353
    [Google Scholar]
  27. Rutberg B., Hoch J. A. 1970; Citric acid cycle: geneenzyme relationships in Bacillus subtilis. Journal of Bacteriology 104:826–833
    [Google Scholar]
  28. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5468
    [Google Scholar]
  29. Sanger F., Coulson A. R., Barrell B. G., Smith A. J. H., Roe B. A. 1980; Cloning in single stranded bacteriophage as an aid to rapid DNA sequencing. Journal of Molecular Biology 143:161–178
    [Google Scholar]
  30. Savva D., Mandelstam J. 1984; Cloning of the Bacillus subtilis spoIIA and spoVA loci in phage ?105DI: It. Journal of General Microbiology 130:2137–2145
    [Google Scholar]
  31. Savva D., Mandelstam J. 1985; Use of cloned spoIIA and spoVA probes to study synthesis of mRNA in wild-type and asporogenous mutants of Bacillus subtilis. In Molecular Biology of Microbial Differentiation pp. 55–59 Edited by Hoch J., Setlow P. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  32. Shine J., Dalgarno L. 1974; The 3? terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proceedings of the National Academy of Sciences of the United States of America 71:1342–1346
    [Google Scholar]
  33. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  34. Staden R. 1984; Graphic methods to determine the function of nucleic acids. Nucleic Acids Research 12:521–538
    [Google Scholar]
  35. Staden R., McLachlan A. 1982; Codon preference and its use in identifying protein coding regions in long DNA sequences. Nucleic Acids Research 10:141–156
    [Google Scholar]
  36. Sterlini J. M., Mandelstam J. 1969; Commitment to sporulation in Bacillus subtilis and its relationship to the development of actinomycin resistance. Biochemical Journal 113:29–37
    [Google Scholar]
  37. Tinoco I., Bover P., Dengler B., Levine M., Uhlenbeck O., Crothers D., Gralla J. 1973; Improved estimation of secondary structure in ribonucleic acids. Nature, New Biology 246:40–41
    [Google Scholar]
  38. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequence of the Ml3 mp and pUC vectors. Gene 33:103–119
    [Google Scholar]
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