SUMMARY: The sporulation gene from was fused to the gene from , so that the transcription of the gene was under the control of the promoter. Production of β-galactosidase, under conditions of sporulation, was then used as an indicator to study the expression of in relation to other sporulation loci. Expression of , which occurred only in the mother cell compartment, was prevented by mutations in all of the stage 0 and stage II loci, and also by , and mutations. By contrast, the last three operons are not needed for expression of , which has previously been shown to be spore-specific. In consequence, a branched pathway of gene expression is proposed. One branch leads to expression of within the spore compartment, the other to expression of in the mother cell.


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