%0 Journal Article %A Clarke, Sandra %A Lopez-Diaz, Isabel %A Mandelstam, Joel %T Use of lacZ Gene Fusions to Determine the Dependence Pattern of the Sporulation Gene spoIID in spo Mutants of Bacillus subtilis %D 1986 %J Microbiology, %V 132 %N 11 %P 2987-2994 %@ 1465-2080 %R https://doi.org/10.1099/00221287-132-11-2987 %I Microbiology Society, %X SUMMARY: The spoIID gene, which is involved in Bacillus subtilis sporulation, was fused to the β-galactosidase gene, lacZ, of Escherichia coli so that the expression of β-galactosidase would be under the control of the spoIID locus. When the fused product was inserted into the B. subtilis chromosome, production of β-galactosidase indicated that the spoIID gene was expressed 1.5 h after the start of sporulation. When the spoIID:: lacZ fusion was inserted into the chromosome of sporulation mutants, all strains carrying spoO lesions and those with mutations in spoIIA, spoIIE and spoIIG loci failed to make β-galactosidase. The proposed provisional order of expression of operons governing stage II is spoIIA [spoIIG, spoIIE] [spoIID, spoIIB, spoIIF]. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-132-11-2987