SUMMARY: The gene, which is involved in sporulation, was fused to the β-galactosidase gene, , of so that the expression of β-galactosidase would be under the control of the locus. When the fused product was inserted into the chromosome, production of β-galactosidase indicated that the gene was expressed 1.5 h after the start of sporulation. When the fusion was inserted into the chromosome of sporulation mutants, all strains carrying lesions and those with mutations in , and loci failed to make β-galactosidase. The proposed provisional order of expression of operons governing stage II is [, ] [, , ].


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