1887

Abstract

SUMMARY: A :: gene fusion has been used to study expression of the operon during sporulation in . β-Galactosidase activity, encoded by the fusion gene, begins to be produced about 2.5 h after the induction of sporulation, well before the phenotypic consequences of mutations are manifested. expression is dependent on all of the known and loci and on some of the “early” loci, but not on “later” loci. Several lines of evidence suggest that expression occurs only in the spore compartment. The implications of this observation for models of the overall regulation of gene expression during sporulation are discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-11-2977
1986-11-01
2021-07-31
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/11/mic-132-11-2977.html?itemId=/content/journal/micro/10.1099/00221287-132-11-2977&mimeType=html&fmt=ahah

References

  1. Andreoli A. J., Saranto J., Baecker P. A., Suehiro S., Escamilla E., Steiner A. 1975; Biochemical properties of forespores isolated from Bacillus cereus. In Spores VI pp. 418–424 Edited by Gerhardt P., Costilow R. N., Sadoff H. L. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  2. Clarke S., Lopez-Diaz I., Mandelstam J. 1986; Use of lacZ gene fusions to determine the dependence pattern of the sporulation gene spoIID in spo mutants of B. subtilis. Journal of General Microbiology 132:2987–2994
    [Google Scholar]
  3. Dawes I. W., Kay D., Mandelstam J. 1969; Sporulation in Bacillus subtilis. Establishment of a time scale for the morphological events. Journal of General Microbiology 56:171–179
    [Google Scholar]
  4. Dion P., Mandelstam J. 1980; Germination properties as marker events characterizing later stages of Bacillus subtilis spore formation. Journal of Bacteriology 141:786–792
    [Google Scholar]
  5. Errington J. 1984; Efficient Bacillus subtilis cloning system using bacteriophage ϕ105J9. Journal of Microbiology 130:2615–2628
    [Google Scholar]
  6. Errington J. 1986; A general method for fusion of the Escherichia coli lacZ gene to chromosomal genes in Bacillus subtilis. Journal of General Microbiology 132:0000–0000
    [Google Scholar]
  7. Errington J., Mandelstam J. 1984; Genetic and phenotypic characterization of a cluster of mutations in the spoVA locus of Bacillus subtilis. Journal of General Microbiology 130:2115–2121
    [Google Scholar]
  8. Errington J., Mandelstam J. 1986; Use of a lacZ gene fusion to determine the dependence pattern of sporulation operon spoIIA in spo mutants of Bacillus subtilis. Journal of General Microbiology 132:2967–2976
    [Google Scholar]
  9. Errington J., Fort P., Mandelstam J. 1985; Duplicated sporulation genes in bacteria. Implica¬tions for simple developmental systems. FEBS Letters 188:184–188
    [Google Scholar]
  10. Fort P., Errington J. 1985; Nucleotide sequence and complementation analysis of a polycistronic sporulation operon, spoVA, in Bacillus subtilis. Journal of General Microbiology 131:1091–1105
    [Google Scholar]
  11. Hames B. D. 1981; An introduction to polyacrylamide gel electrophoresis. In Gel Electrophoresis of Proteins: a Practical Approach pp. 1–91 Edited by Hames B. D., Rickwood D. Oxford Washington, DC: IRL Press.;
    [Google Scholar]
  12. Hranueli D., Piggot P. J., Mandelstam J. 1974; Statistical estimate of the total number of operons specific for Bacillus subtilis sporulation. Journal of Bacteriology 119:684–690
    [Google Scholar]
  13. James W., Mandelstam J. 1985; spoVIC, a new sporulation locus in Bacillus subtilis affecting spore coats, germination and the rate of sporulation. Journal of General Microbiology 131:2409–2419
    [Google Scholar]
  14. Jenkinson H. F., Kay D., Mandelstam J. 1980; Temporal dissociation of late events in Bacillus subtilis sporulation from expression of the genes that determine them. Journal of Bacteriology 141:793–805
    [Google Scholar]
  15. Jenkinson H. F., Sawyer W. D., Mandelstam J. 1981; Synthesis and order of assembly of spore coat proteins in Bacillus subtilis. Journal of General Microbiology 123:1–16
    [Google Scholar]
  16. Johnson W. C., Mahler I., Phillips K., Tipper D. J. 1985; Transcriptional control of synthesis of acid-soluble proteins in sporulating Bacillus subtilis. Journal of Bacteriology 163:543–551
    [Google Scholar]
  17. Kalnins A., Otto K., Ruther U., Muller-Hill B. 1983; Sequence of the lacZ gene of Escherichia coli. EMBO Journal 2:593–597
    [Google Scholar]
  18. Laemmli U. K., Favre M. 1973; Maturation of the head of bacteriophage T4.I. DNA packaging events. Journal of Molecular Biology 80:575–599
    [Google Scholar]
  19. Lamont I. L., Mandelstam J. 1984; Identification of a new sporulation locus, spoIIIF, in Bacillus subtilis. Journal of General Microbiology 130:1253–1261
    [Google Scholar]
  20. Lencastre H., de Piggot P. J. 1979; Identification of different sites of expression for spo loci by transformation of Bacillus subtilis. Journal of General Microbiology 114:377–389
    [Google Scholar]
  21. Murrell W. G., Ohye D. F., Gordon R. A. 1969; Cytological and chemical structure of the spore. In Spores IV Edited by Campbell L. L. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  22. Nakayama T., Irikura M., Kurogi Y., Matsuo H. 1981; Purification and properties of RNA polymerases from mother cells and forespores of sporulating cells of Bacillus subtilis. Journal of Biochemistry 89:1681–1691
    [Google Scholar]
  23. Piggot P. J., Coote J. G. 1976; Genetic aspects of bacterial endospore formation. Bacteriological Reviews 40:908–962
    [Google Scholar]
  24. Piggot P. J., Moir A., Smith D. A. 1981; Advances in the genetics of Bacillus subtilis differen¬tiation. In Sporulation and Germination pp. 29–39 Edited by Levinson H. S., Sonenshein A. L., Tipper D. J. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  25. Powell J. F., Hunter J. R. 1955; Spore germination in the genus Bacillus: the modification of germination requirements as a result of preheating. Journal of General Microbiology 13:59–67
    [Google Scholar]
  26. Rosenbluh A., Banner C. D., Losick R., Fitz-James P. C. 1981; Identification of a new developmental locus in Bacillus subtilis by construction of a deletion mutation in a cloned gene under sporulation control. Journal of Bacteriology 148:341–351
    [Google Scholar]
  27. Savva D., Mandelstam J. 1984; Cloning of the Bacillus subtilis spoIIA and spoVA loci in phage ϕ105DI:lt. Journal of General Microbiology 130:2137–2145
    [Google Scholar]
  28. Savva D., Mandelstam J. 1985; Use of cloned spoIIA and spo VA probes to study synthesis of mRNA in wild-type and asporogenous mutants of Bacillus subtilis. In Molecular Biology of Microbial Differentiation pp. 55–59 Edited by Setlow P., Hoch J. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  29. Savva D., Mandelstam J. 1986; Synthesis of spoIIA and spoVA mRNA in Bacillus subtilis. Journal of General Microbiology 132:3005–3011
    [Google Scholar]
  30. Setlow P. 1981; Biochemistry of bacterial forespore development and spore germination. In Sporulation and Germination pp. 13–28 Edited by Levinson H. S., Sonenshein A. L., Tipper D. J. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  31. Turner S. M., Errington J., Mandelstam J. 1986; Use of a lacZ gene fusion to determine the dependence pattern of the sporulation gene spoIIIC in spo mutants of Bacillus subtilis: a branched pathway of expression of sporulation operons. Journal of General Microbiology 132:2995–3003
    [Google Scholar]
  32. Wood D. A. 1972; Properties and time of synthesis of alkali-soluble protein of the spore coat. Biochemical Journal 130:505–514
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-11-2977
Loading
/content/journal/micro/10.1099/00221287-132-11-2977
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error