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Volume 132, Issue 10

Bacteriophages F , SR, SF: Phages Which Adsorb to Pili Encoded by Plasmids of the S-Complex Free

Abstract

SUMMARY: Phage F is an RNA-containing phage which plates only on strains carrying the plasmid EDP208, a pilus derepressed derivative of the unique incompatibility plasmid F A host range mutant, phage F , was selected which plated on strains carrying the ungrouped plasmid pPLS:: Tn5 and lysed strains carrying another ungrouped plasmid TP224::Tn10 or the Com9 plasmid R71. An RNA-containing phage, SR, was isolated from sewage on bacteria harbouring plasmid pPLS::Tn5. It was antigenically distinct from the above two phages but had the same host range as phage F Phages F and SR adsorbed unevenly to the shafts of the conjugative pili. Another phage, SF, was filamentous and plated or propagated on strains carrying any of the above plasmids as well as on strains harbouring IncD or F-complex plasmids. Plasmids TP224::Tn10 and pPLS::Tn5 were compatible with representative plasmids of all Inc groups also encoding thick flexible pili. The four plasmids EDP208, R71, TP224::Tn10 and pPLS::Tn5 were compatible with one another except for the reaction of TP224::Tn10 in the presence of pPLS::Tn5 which was slightly ambiguous. The host ranges of the bacteriophages, together with the serological relatedness of the thick flexible pili determined by these four compatible plasmids, suggested that they constitute a new complex, here designated S.

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© Society for General Microbiology 1986
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1986-10-01
2024-04-25
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References

  1. Adams M. H. 1950; Methods of study of bacterial viruses. Methods in Medical Research 2:1–73
     [Google Scholar]
  2. Armstrong G. D., Frost L. S., Sastry P. A., Paranchych W. 1980; Comparative biochemical studies on F and EDP208 conjugative pili. Journal of Bacteriology 141:333–341
     [Google Scholar]
  3. Bradley D. E. 1978; Determination of pili by bacterial drug resistance plasmids assigned to the X incompatibility group. In Pili pp 355–371 Edited by Bradley D. E., Raizen E., Fives-Taylor P., Ou J. Washington, DC: International Conferences on Pili;
     [Google Scholar]
  4. Bradley D. E. 1980; Morphological and serological relationships of conjugative pili. Plasmid 4:155–169
     [Google Scholar]
  5. Bradley D. E. 1981; Conjugative pili of plasmids in Escherichia coli K-12 and Pseudomonas species. In Molecular Biology, Pathogenicity, and Ecology of Bacterial Plasmids pp 217–226 Edited by Levy S. B., Clowes R. C., Koenig E. L. New York: Plenum Press;
     [Google Scholar]
  6. Bradley D. E. 1984; Characteristics and function of thick and thin conjugative pili determined by transfer derepressed plasmids of incompatibility groups I1, I2, I5, B, K and Z. Journal of General Microbiology 130:1489–1502
     [Google Scholar]
  7. Bradley D. E. 1985; Transfer systems of K88 and K99 plasmids. Plasmid 13:118–128
     [Google Scholar]
  8. Bradley D. E., Fleming J. 1983; Incompatibility group identification for repressed plasmids using host cell lysis by specific bacteriophages. Journal of Microbiological Methods 1:171–176
     [Google Scholar]
  9. Bradley D. E., Coetzee J. N., Bothma T., Hedges R. W. 1981a; Phage F0lac: an F0lac plasmid-dependent bacteriophage. Journal of General Microbiology 126:405–411
     [Google Scholar]
  10. Bradley D. E., Coetzee J. N., Bothma T., Hedges R. W. 1981b; Phage t: a group T plasmid-dependent bacteriophage. Journal of General Microbiology 126:397–403
     [Google Scholar]
  11. Bradley D. E., Hughes V. M., Richards H., Datta N. 1982a; R plasmids of a new incompatibility group determine constitutive production of H pili. Plasmid 7:230–238
     [Google Scholar]
  12. Bradley D. E., Sirgel F. A., Coetzee J. N., Hedges R. W., Coetzee W. F. 1982b; Phages C-2 and J: IncC and IncJ plasmid-dependent phages, respectively. Journal of General Microbiology 128:2485–2498
     [Google Scholar]
  13. Chabbert Y. A., Scavizzi M. R., Witchitz J. L., Gerbaud G. R., Bouanchaud D. H. 1972; Incompatibility groups and the classification of fi − resistance factors. Journal of Bacteriology 112:666–675
     [Google Scholar]
  14. Coetzee J. N., Datta N., Hedges R. W. 1972; R factors from Proteus rettgeri. Journal of General Microbiology 72:543–552
     [Google Scholar]
  15. Coetzee J. N., Lecatsas G., Coetzee W. F., Hedges R. W. 1979; Properties of R plasmid R772 and the corresponding pilus-specific phage PR772. Journal of General Microbiology 110:263–273
     [Google Scholar]
  16. Coetzee J. N., Bradley D. E., Hedges R. W. 1982; Phages Iα and I2-2: IncI plasmid-dependent bacteriophages. Journal of General Microbiology 128:2797–2804
     [Google Scholar]
  17. Coetzee J. N., Bradley D. E., Hedges R. W., Fleming J., Lecatsas G. 1983; Bacteriophage M: an incompatibility group M plasmid-specific phage. Journal of General Microbiology 129:2271–2276
     [Google Scholar]
  18. Coetzee J. N., Bradley D. E., Fleming J., du Toit L., Hughes V. M., Hedges R. W. 1985a; Phage pilHα: a phage which adsorbs to IncHI and IncHII plasmid-coded pili. Journal of General Microbiology 131:1115–1121
     [Google Scholar]
  19. Coetzee J. N., Bradley D. E., Lecatsas G., du Toit L., Hedges R. W. 1985b; Bacteriophage D: an IncD group plasmid-specific phage. Journal of General Microbiology 131:3375–3383
     [Google Scholar]
  20. Cramer J. H., Sinsheimer R. L. 1971; Replication of bacteriophage MS2. X. Phage-specific ribo-nucleoprotein particles found in MS2-infected Escherichia coli. Journal of Molecular Biology 62:189–214
     [Google Scholar]
  21. Datta N. 1975; Epidemiology and classification of plasmids. In Microbiology–1974 pp 9–15 Edited by Schlessinger D. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  22. Datta N. 1979; Plasmid classification: incompatibility grouping. In Plasmids of Medical, Environmental and Commercial Importance pp 3–12 Edited by Timmis K. N., Puhler A. Amsterdam: Elsevier/North Holland Biomedical Press;
     [Google Scholar]
  23. Datta N., Hedges R. W. 1972; R factors identified in Paris, some conferring gentamicin resistance, constitute a new compatibility group. Annales de l’Institut Pasteur 123:879–883
     [Google Scholar]
  24. Datta N., Dacey S., Hughes V., Knight S., Richards H., Williams G., Case well M., Shannon K. P. 1980; Distribution of genes for trimethoprim and gentamicin resistance in bacteria and their plasmids in a general hospital. Journal of General Microbiology 118:495–508
     [Google Scholar]
  25. Davis J. E., Strauss H. J., Sinsheimer R. L. 1961; Bacteriophage MS2: another RNA phage. Science 134:1427
     [Google Scholar]
  26. Dennison S. 1972; A naturally occurring R factor derepressed for pilus synthesis, belong to the same compatibility groups as the sex factor F of Escherichia coli K12. Journal of Bacteriology 109:416–422
     [Google Scholar]
  27. Dennison S., Hedges R. W. 1972; Host specificities of RNA phages. Journal of Hygiene 70:55–61
     [Google Scholar]
  28. Falkow S., Baron L. S. 1962; Episomic element in a strain of Salmonella typhosa. Journal of Bacteriology 84:581–589
     [Google Scholar]
  29. Fiers W. 1979; Structure and function of RNA bacteriophages. In Comprehensive Virology vol 13 pp 69–204 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
     [Google Scholar]
  30. Frydman A., Meynell E. 1969; Interactions between de-repressed F-like R factors and wild type colicin B factors: superinfection immunity and repressor susceptibility. Genetical Research 14:315–332
     [Google Scholar]
  31. Hedges R. W., Datta N. 1972; R124, an fi+ R factor of a new compatibility class. Journal of General Microbiology 71:403–405
     [Google Scholar]
  32. Jacob A. E., Shapiro J. A., Yamamoto L., Smith D. I., Cohen S. N., Berg D. 1977; Plasmids studied in Escherichia coli and other enteric bacteria. In DNA Insertion Elements, Plasmids and Episomes pp 607–638 Edited by Bukhari A. I., Shapiro J. A., Adhya S. L. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  33. Jacob F., Adelberg E. A. 1959; Transfert de charactères génétiques par incorporation au facteur d’Escherichia coli. Compte rendu hebdomadaire des seances de l’Academie des Sciences 249:159–193
     [Google Scholar]
  34. Lawn A. M., Meynell G. G., Meynell E., Datta N. 1967; Sex pili and the classification of sex factors in the Enterobacteriaceae. Nature, London 216:343–346
     [Google Scholar]
  35. Marvin D. A., Hoffmann-Berling H. 1963; Physical and chemical properties of two new small bacteriophages. Nature, London 197:517–518
     [Google Scholar]
  36. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. 1976; Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. Journal of Bacteriology 127:1529–1537
     [Google Scholar]
  37. Meynell E. 1978; Experiments with sex pili: an investigation of the characters of function of F-like and I-like sex pili based on their reactions with antibody and phage. In Pili pp 207–233 Edited by Bradley D. E., Raizen E., Fives-Taylor P., Ou J. Washington, DC: International Conferences on Pili;
     [Google Scholar]
  38. Meynell E., Meynell G. G., Datta N. 1968; Phylogenetic relationships of drug resistance factors and other transmissible bacterial plasmids. Bacteriological Reviews 32:35–83
     [Google Scholar]
  39. Scavizzi M. R. 1973; Nouveaux groupes d’incompatibilité des plasmides. Intérêt dans les épidémies de crèche à Escherichia coli 01111: B4. New incompatibility groups of plasmids. Interest on 01111: B4 Escherichia coli outbreaks observed in pediatrics department. Annales de l’Institut Pasteur/Microbiologie 124B:153–167
     [Google Scholar]
  40. Scotland S. M., Gross R. J., Cheasty T., Rowe B. 1979; The occurrence of plasmids carrying genes for both enterotoxin production and drug resistance in Escherichia coli of human origin. Journal of Hygiene 83:531–538
     [Google Scholar]
  41. Shipley P. L., Gyles C. L., Falkow S. 1978; Characterization of plasmids that encode for the K88 colonization antigen. Infection and Immunity 20:559–566
     [Google Scholar]
  42. Silverman P. M., Rosenthal S., Mobach H., Valentine R. C. 1968; Two new classes of F-pili mutants of Escherichia coli resistant to infection by the male specific bacteriophage f2. Virology 36:142–146
     [Google Scholar]
  43. Taylor D. E., Grant R. B. 1977; R plasmids of the S incompatibility group belong to the H2 incompatibility group. Antimicrobial Agents and Chemotherapy 12:431–434
     [Google Scholar]
  44. Watanabe I., Miyake T., Sakurai T., Shiba T., Ohno T. 1967; Isolation and grouping of RNA phages. Proceedings of the Japan Academy 43:204–209
     [Google Scholar]
  45. Zinder N. D. 1965; RNA phages. Annual Review of Biochemistry 33:455–476
     [Google Scholar]
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Bacteriophages Folac h, SR, SF: Phages Which Adsorb to Pili Encoded by Plasmids of the S-Complex
Microbiology 132, 2907 (1986); https://doi.org/10.1099/00221287-132-10-2907
/content/journal/micro/10.1099/00221287-132-10-2907
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