1887

Abstract

SUMMARY: Four strains of serotype differing in glucosyltransferase (GTase) and fructosyltransferase (FTase) activities were examined. These strains had been made resistant to streptomycin. FTase activity of an clinical variant, MT6801R, which forms large mucoid colonies on sucrose-containing agar, was considerably higher than that of a typical serotype strain, MT8148R, which forms small, rough colonies on the same agar. Two mutants, NG14 and NG7183, were induced from strain MT6801R by -methyl--nitro--nitrosoguanidine, and were found to be streptomycin-resistant. GTase and FTase activities of mutant NG14 were similar to those of the typical serotype strain, while in mutant NG7183 the two enzyme activities were very low. Growing cells of these strains (except NG7183) adhered firmly to a glass surface in sucrose broth. Resting cells of all strains attached in small numbers to saliva-coated hydroxyapatite in the absence of sucrose. On the other hand, the presence of sucrose markedly enhanced the attachment of cells of strains MT8148R, MT6801R and NG14, but not NG7183. Cell-surface hydrophobicity and acid production of all strains were similar. Both strain MT8148R and NG14 colonized tooth surfaces and produced significant dental caries in specific-pathogen-free rats. Strain MT6801R had lower colonization ability and cariogenicity when compared with strains MT8148R and NG14. Furthermore, mutant NG7183 was able to colonize the tooth surfaces in small numbers, but failed to cause dental caries. These results indicate that sucrose-dependent cell adherence mediated by glucan synthesis is necessary for the accumulation of serotype cells on the tooth surface and the induction of dental caries.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-10-2873
1986-10-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/10/mic-132-10-2873.html?itemId=/content/journal/micro/10.1099/00221287-132-10-2873&mimeType=html&fmt=ahah

References

  1. Adelberg E. A., Mandel M., Chen G. C. C. 1965; Optimal conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine in Escherichia coli K12. Biochemical and Biophysical Research Communications 18:5–6
    [Google Scholar]
  2. Burnette W. N. 1981; “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  3. Cohen B., Peach S. L., Russell R. R. B. 1983; Immunization against dental caries. Medical Microbiology 2:255–294
    [Google Scholar]
  4. Eifert R., Rosan B., Golub E. 1984; Optimization of an hydroxyapatite adhesion assay for Streptococcus sanguis. Infection and Immunity 44:287–291
    [Google Scholar]
  5. Fives-Taylor P. M., Thompson D. W. 1985; Surface properties of Streptococcus sanguis FW213 mutants nonadherent to saliva-coated hydroxyapatite. Infection and Immunity 47:752–759
    [Google Scholar]
  6. Freedman M. L., Tanzer J. M. 1974; Dissociation of plaque formation from glucan-induced agglutination in mutants of Streptococcus mutans. Infection and Immunity 10:189–196
    [Google Scholar]
  7. Furuta T., Nisizawa T., Chiba J., Hamada S. 1983; Production of monoclonal antibody against a glucosyltransferase of Streptococcus mutans 6715. Infection and Immunity 41:872–875
    [Google Scholar]
  8. Gibbons R. J. 1984; Adherent interaction which may affect microbial ecology in the mouth. Journal of Dental Research 63:378–385
    [Google Scholar]
  9. Gibbons R. J., Etherden I. 1983; Comparative hydrophobicities of oral bacteria and their adherence to salivary pellicles. Infection and Immunity 41:1190–1196
    [Google Scholar]
  10. Gutmann I., Wahlefeld A. W. 1974; l-(+)-Lactate; determination with lactate dehydrogenase and NAD. In Methods of Enzymatic Analysis vol 3 pp 1464–1468 Edited by Bergmeyer H. U. New York & London: Academic Press;
    [Google Scholar]
  11. Hamada S. 1977; New glucan synthesis as a prerequisite for adherence of Streptococcus mutans to smooth surfaces. Microbios Letters 5:141–146
    [Google Scholar]
  12. Hamada S., Slade H. D. 1980; Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiological Reviews 44:331–384
    [Google Scholar]
  13. Hamada S., Torii M., Kotani S., Tsuchitani Y. 1981; Adherence of Streptococcus sanguis clinical isolates to smooth surfaces and interaction of the isolates with Streptococcus mutans glucosyltransferase. Infection and Immunity 32:364–372
    [Google Scholar]
  14. Handel E. V. 1967; Determination of fructose and fructose-yielding carbohydrates with cold anthrone. Analytical Biochemistry 19:193–194
    [Google Scholar]
  15. Keyes P. H. 1944; A method of recording and scoring gross carious lesions in the molar teeth of Syrian hamsters. Journal of Dental Research 23:439–444
    [Google Scholar]
  16. Keyes P. H., Jordan H. V. 1964; Periodontal lesions in the Syrian hamster. III. Findings related to an infectious and transmissible component. Archives of Oral Biology 9:377–400
    [Google Scholar]
  17. Koga T., Inoue M. 1978; Cellular adherence, glucosyltransferase adsorption, and glucan synthesis of Streptococcus mutans AHT mutants. Infection and Immunity 19:402–410
    [Google Scholar]
  18. Koga T., Inoue M. 1981; Inactivation of d-glucosyltransferases of oral Streptococcus mutans and Streptococcus sanguis by photochemical oxidation. Carbohydrate Research 93:125–133
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  20. McBride B. C., Song M., Krasse B., Olsson J. 1984; Biochemical and immunological differences between hydrophobic and hydrophilic strains of Streptococcus mutans. Infection and Immunity 44:68–75
    [Google Scholar]
  21. Mukasa H., Slade H. D. 1973; Mechanism of adherence of Streptococcus mutans to smooth surfaces. I. Roles of insoluble dextran-levan synthetase enzymes and cell wall polysaccharide antigen in plaque formation. Infection and Immunity 8:555–562
    [Google Scholar]
  22. Nesbitt W., Doyle R. J., Taylor K. G. 1982; Hydrophobic interaction and the adherence of Streptococcus sanguis to hydroxylapatite. Infection and Immunity 38:637–644
    [Google Scholar]
  23. Okahashi N., Asakawa H., Koga T., Masuda N., Hamada S. 1984; Clinical isolates of Streptococcus mutans serotype c with altered colony morphology due to fructan synthesis. Infection and Immunity 44:617–622
    [Google Scholar]
  24. Olsson J., Westergren G. 1972; Hydrophobic surface properties of oral streptococci. FEMS Microbiology Letters 15:319–323
    [Google Scholar]
  25. Ooshima T., Izumitani A., Sobue S., Okahashi N., Hamada S. 1983; Non-cariogenicity of the disaccharide palatinose in experimental dental caries of rats. Infection and Immunity 39:43–49
    [Google Scholar]
  26. Rosenberg M., Gutnick D., Rosenberg E. 1980; Adherence of bacteria to hydrocarbons: a simple method for measuring cell-surface hydrophobicity. FEMS Microbiology Letters 9:29–33
    [Google Scholar]
  27. Russell M. W., Zanders E. D., Bergmeier L. A., Lehner T. 1980; Affinity purification and characterization of protease-susceptible antigen I of Streptococcus mutans. Infection and Immunity 29:999–1006
    [Google Scholar]
  28. Russell R. R. B. 1979a; Glucosyltransferases of Streptococcus mutans strain Ingbritt. Microbios 23:135–146
    [Google Scholar]
  29. Russell R. R. B. 1979b; Wall-associated protein antigens of Streptococcus mutans. Journal of General Microbiology 114:109–115
    [Google Scholar]
  30. Sato S., Koga T., Inoue M. 1984; Isolation and some properties of extracellular d-glucosyltransferases and d-fructosyltransferases from Streptococcus mutans serotypes c, e, and f. Carbohydrate Research 134:293–304
    [Google Scholar]
  31. Smith D. J., Taubman M. A. 1977; Antigenic relatedness of glucosyltransferase enzymes from Streptococcus mutans. Infection and Immunity 15:91–103
    [Google Scholar]
  32. Tsumori H., Shimamura A., Mukasa H. 1983; Comparative study of Streptococcus mutans extracellular glycosyltransferases by isoelectric focusing. Journal of General Microbiology 129:3261–3269
    [Google Scholar]
  33. Weiss E., Rosenberg M., Judes H., Rosenberg E. 1982; Cell-surface hydrophobicity of adherent oral bacteria. Current Microbiology 7:125–128
    [Google Scholar]
  34. Westergren G., Olsson J. 1983; Hydrophobicity and adherence of oral streptococci after repeated subculture in vitro. Infection and Immunity 40:432–435
    [Google Scholar]
  35. Yakushiji T., Inoue M., Koga T. 1984; Interserotype comparison of polysaccharides produced by extracellular enzymes of Streptococcus mutans. Carbohydrate Research 127:253–266
    [Google Scholar]
  36. Zacharius R. M., Zell T. E., Morrison J. H., Woodlock J. J. 1969; Glycoprotein staining following electrophoresis on acrylamide gels. Analytical Biochemistry 30:148–152
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-10-2873
Loading
/content/journal/micro/10.1099/00221287-132-10-2873
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error