RT Journal Article SR Electronic(1) A1 Kohler-Staub, Doris A1 Hartmans, Sybe A1 Gälli, Rene A1 Suter, Franz A1 Leisinger, ThomasYR 1986 T1 Evidence for Identical Dichloromethane Dehalogenases in Different Methylotrophic Bacteria JF Microbiology, VO 132 IS 10 SP 2837 OP 2843 DO https://doi.org/10.1099/00221287-132-10-2837 PB Microbiology Society, SN 1465-2080, AB SUMMARY: The dichloromethane dehalogenases of four facultative methylotrophs that mineralize dichloromethane were compared. Similar levels of specific activity and a similar subunit M r of the dehalogenases were observed in crude extracts of the four strains. Immunodiffusion analysis and quantitative immunoprecipitation indicated that the dehalogenases closely resemble each other. This was confirmed when the N-terminal amino acid sequences of the four dichloromethane dehalogenases were found to be identical. Some variation was observed with respect to the specificity of the inducers for dichloromethane dehalogenase. In the two Hyphomicrobium strains examined 1,1-dichloroethane and 1,2-dichloroethane were effective gratuitous inducers whereas these compounds exhibited only a marginal inducing effect in the two Pseudomonas strains. The results of this comparative study are compatible with the view that dichloromethane dehalogenase represents a recently evolved enzyme which was horizontally distributed by gene transfer., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-132-10-2837