1887

Abstract

SUMMARY: A glutamine synthetase (GS) gene, , from was cloned on a recombinant plasmid pJS139 which enabled deletion mutants to utilize (NH)SO as a sole source of nitrogen. DNA homology was not detected between the gene and the gene. The cloned gene was expressed from its own promoter and was subject to nitrogen repression in , but it was not able to activate histidase activity in an deletion mutant containing the operon. The GS produced by pJS139 in was purified: it had an apparent subunit of approximately 75000, which is larger than that of any other known bacterial GS. There was very slight antigenic cross-reactivity between antibodies to the purified cloned GS and the GS subunit of wild-type

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-10-2827
1986-10-01
2021-07-30
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/10/mic-132-10-2827.html?itemId=/content/journal/micro/10.1099/00221287-132-10-2827&mimeType=html&fmt=ahah

References

  1. Anderson B. L., Bills M. M., Egerton J. R., Mattick J. S. 1984; Cloning and expression in Escherichia coli of the gene encoding the structural sub-unit of Bacteroides nodosus fimbriae. Journal of Bacteriology 160:748–754
    [Google Scholar]
  2. Backman K., Chen Y.-M., Magasanik B. 1981; Physical and genetic characterization of the glnA–glnG region of the Escherichia coli chromosome. Proceedings of the National Academy of Sciences of the United States of America 78:3743–3747
    [Google Scholar]
  3. Barros M. E. C., Rawlings D. E., Woods D. R. 1985; Cloning and expression of the Thiobacillus ferrooxidans glutamine synthetase gene in Escherichia coli. Journal of Bacteriology 164:1386–1389
    [Google Scholar]
  4. Barros M. E. C., Rawlings D. E., Woods D. R. 1986; Purification and regulation of a cloned Thiobacillus ferrooxidans glutamine synthetase cloned in Escherichia coli. Journal of General Microbiology 132:1989–1995
    [Google Scholar]
  5. Bender R. A., Janssen K. A., Resnick A. D., Blumenberg M., Foor F., Magasanik B. 1977; Biochemical parameters of glutamine synthetase from Klebsiella aerogenes. Journal of Bacteriology 129:1001–1009
    [Google Scholar]
  6. Bhandari B., Vairinhos F., Nicholas D. J. D. 1983; Some properties of glutamine synthetase from Rhizobium japonicum CC705 and CC723. Archives of Microbiology 136:84–88
    [Google Scholar]
  7. Bodasing S. J., Brandt P. W., Robb F. T., Woods D. R. 1985; Purification and regulation of glutamine synthetase in a collagenolytic Vibrio alginolyticus strain. Archives of Microbiology 140:369–374
    [Google Scholar]
  8. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  9. Crosby B., Collier B. 1984; Cloning and expression in Escherichia coli of cellulase genes from Bacteroides succinogenes. Applied Science 27:573–576
    [Google Scholar]
  10. Davis R. W., Botstein D., Roth J. R. 1980 Advanced Bacterial Genetics Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Deuel T. F., Ginsburg A., Yeh J., Shelton E., Stadman E. R. 1970; Bacillus subtilis glutamine synthetase. Journal of Biological Chemistry 245:5195–5205
    [Google Scholar]
  12. Elleman T. C., Hoyne P. A. 1984; Isolation of the gene encoding pilin of Bacteroides nodosus (strain 198): the causal organism of ovine foot rot. FEBS Letters 173:103–107
    [Google Scholar]
  13. Garcia E., Bancroft S., Rhee S. G., Kustu S. 1977; The product of a newly identified gene, glnF, is required for synthesis of glutamine synthetase in Salmonella. Proceedings of the National Academy of Sciences of the United States of America 74:1662–1666
    [Google Scholar]
  14. Guiney D. G., Hasegawa P., Davis C. E. 1984; Plasmid transfer from Escherichia coli to Bacteroides fragilis: differential expression of antibiotic resistance phenotypes. Proceedings of the National Academy of Sciences of the United States of America 81:7203–7206
    [Google Scholar]
  15. Guthrie E. P., Shoemaker N. B., Salyer A. A. 1985; Cloning and expression in Escherichia coli of a gene coding for a chondroitin lyase from Bacteroides thetaiotaomicron. Journal of Bacteriology 164:510–515
    [Google Scholar]
  16. Ish-Horowitz D., Burke J. F. 1981; Rapid and efficient cosmid cloning. Nucleic Acids Research 9:2989–2998
    [Google Scholar]
  17. Johnson D. A., Gautsch J. W., Sportsman J. R., Elder J. H. 1984; Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose. Genetic Analytical Techniques 1:3–8
    [Google Scholar]
  18. Kasper D. J., Onderdonk A. B., Polk B. F., Bartlett J. G. 1979; Surface antigens as virulence factors in infection with Bacteroides fragilis. Reviews of Infectious Diseases 1:278–288
    [Google Scholar]
  19. Kustu S., McFarland N., Hui S. P., Esmon B., Ames G. F. 1979; Nitrogen control in Salmonella: co-regulation of synthesis of glutamine synthetase and amino acid transport systems. Journal of Bacteriology 138:218–234
    [Google Scholar]
  20. Laemmli U. K. 1970; Cleavage of the structural proteins during assembly of the head of bacteriophage T4. Nature, London 277:680–685
    [Google Scholar]
  21. Magasanik B. 1982; Genetic control of nitrogen assimilation in bacteria. Annual Review of Genetics 16:135–168
    [Google Scholar]
  22. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  24. McFarland N., McCarter L., Artz S., Kustu S. 1981; Nitrogen regulation locus glnR of enteric bacteria is composed of cistrons ntrB and ntrC: identification of their products. Proceedings of the National Academy of Sciences of the United States of America 78:2135–2139
    [Google Scholar]
  25. Meinkoth J., Wahl G. 1984; Hybridization of nucleic acids immobilized on solid supports. Analytical Biochemistry 138:267–284
    [Google Scholar]
  26. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Mossie K. G., Jones D. T., Robb F. T., Woods D. R. 1979; Characterization and mode of action of a bacteriocin produced by a Bacteroides fragilis strain. Antimicrobial Agents and Chemotherapy 16:724–730
    [Google Scholar]
  28. O’Farrel P. H. 1975; High resolution two-dimensional clectrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
    [Google Scholar]
  29. Pahel G., Tyler B. 1979; A new glnA-linked regulatory gene for glutamine synthetase in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 76:4544–4548
    [Google Scholar]
  30. Remaut E., Tsao H., Fiers W. 1983; Improved plasmid vectors with thermo-inducible expression and temperature regulated runaway replication. Gene 22:103–113
    [Google Scholar]
  31. Rybicki E. P., von Wechmar M. B. 1982; Enzyme assisted immune detection of plant virus proteins electroblotted onto nitrocellulose paper. Journal of Virological Methods 5:267–278
    [Google Scholar]
  32. Smith G. E., Summers M. D. 1980; Recovery of DNA from gels. Analytical Biochemistry 109:123–129
    [Google Scholar]
  33. Smith G. R., Halpern Y. S., Magasanik B. 1971; Genetic and metabolic control of enzymes responsible for histidine degradation in Salmonella typhimurium. Journal of Biological Chemistry 246:3320–3329
    [Google Scholar]
  34. Southern J. A., Katz W., Woods D. R. 1984; Purification and properties of a cell-bound bacteriocin from a Bacteroides fragilis strain. Antimicrobial Agents and Chemotherapy 25:253–257
    [Google Scholar]
  35. Stadtman E. R., Ginsburg A. 1974; The glutamine synthetase of Escherichia coli: structure and control. In The Enzymes, 3rd edn. vol. 10 pp. 755–807 Edited by Boyer P. D. New York: Academic Press;
    [Google Scholar]
  36. Streicher S. L., Tyler B. 1980; Purification of glutamine synthetase from a variety of bacteria. Journal of Bacteriology 142:69–78
    [Google Scholar]
  37. Sykes R. B., Nordström K. 1972; Microiodometric determination of β-lactamase activity. Antimicrobial Agents and Chemotherapy 1:94–99
    [Google Scholar]
  38. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  39. Tronick S. R., Ciardi J. E., Stadtman E. R. 1973; Comparative biochemical and immunological studies of bacterial glutamine synthetases. Journal of Bacteriology 115:858–868
    [Google Scholar]
  40. Tuli R., Fisher R., Haselkorn R. 1982; The ntr genes of Escherichia coli activate the hut and nif operons of Klebsiella pneumoniae. Gene 19:109–116
    [Google Scholar]
  41. Weir D. M. (Editor) 1973 Handbook of Experimental Immunology, 2nd edn. vol. 1 pp. 13–19 Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  42. Zabeau M., Stanley K. K. 1982; Enhanced expression of cro-β-galactosidase fusion proteins under the control of the PR promoter of the bacteriophage lambda. EMBO Journal 1:1217–1224
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-10-2827
Loading
/content/journal/micro/10.1099/00221287-132-10-2827
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error