SUMMARY: Sexual stimulation increases the β-carotene content of the fungus The same effect is observed in single cultures exposed to natural and synthetic trisporates and in intersexual heterokaryons. Synthetic racemic (9Z)-methyl trisporate B, a trisporate precursor made by cultures of the (+) mating type, stimulates carotenogenesis only in (-) cultures. Synthetic racemic (9Z)-methyl trisporate C is less effective and the corresponding all-() isomers and other related compounds are inactive. Sexual stimulation of carotenogenesis is additive with the stimulations induced by light, retinol, dimethyl phthalate, and mutation of the gene


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