1887

Abstract

SUMMARY: The cyanobacterium was found to have nickel-dependent urease activity. Addition of nickel to a nickel-free culture led to recovery of urease activity. This was not prevented by chloramphenicol. Cell-free extracts of nickel-depleted cells did not regain activity upon addition of Ni. Enzyme synthesis was constitutive; similar urease activities occurred in urea-, NO - or NH-grown cultures or those grown under N-fixing conditions. The urease activity had hyperbolic kinetics, with an apparent with respect to urea of 1.3 ± 0.6 () m. Enzyme activity was enhanced up to two fold by MgSO and MnSO at pH 7.5. At 10 m-urea, cells hydrolysed urea and excreted NH in excess of biosynthetic requirements.

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/content/journal/micro/10.1099/00221287-132-10-2749
1986-10-01
2021-10-27
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